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The map label for this gene is fumB [H]

Identifier: 59711787

GI number: 59711787

Start: 1310136

End: 1311665

Strand: Direct

Name: fumB [H]

Synonym: VF_1180

Alternate gene names: 59711787

Gene position: 1310136-1311665 (Clockwise)

Preceding gene: 59711781

Following gene: 59711788

Centisome position: 45.22

GC content: 42.16

Gene sequence:

>1530_bases
ATGATTAACGTTCACCAAGAAACATTGATTCGTAATGAAGATGTGATCAGTTCTGTAGCCGATGCTCTGCAATATATATC
TTATTATCATCCGCTAGATTTTGTTCAAGCACTTGAAAAAGCGTATCACCGAGAAGAAAGCCAAGCTGCAAAAGATGCGC
TTGCACAAATCCTAATTAATTCACGTATGTCAGCAGAGGGGCGTCGTCCTATCTGTCAAGATACGGGAATTGTAACTTGT
TTTGTTAATATTGGTATGAACGTTAGGTGGGAAGGAGACTTAACGGTTCAACAAATGATTGATGAAGGTACTCGTCAAGC
TTATACCAATCCTGACAATCCATTAAGGGCATCCGTATTGTCTGATCCGGCTGGTAAACGTATAAACACAAAAGATAATA
CGCCAGCAGTGGTTCATATTAATATGGTTCCGGGAAACAAAGTTGAGATTCAAATTGCTGCAAAAGGTGGCGGCTCTGAG
AATAAAACTAAAATGGTAATGTTAAACCCATCAGATGATATTGCTGAGTGGGTAGAAAAGACGCTTCCAACAATGGGTGC
GGGTTGGTGTCCTCCAGGTATGCTTGGTATAGGTGTTGGCGGTACGGCTGAGAAAGCAGCTGTATTAGCAAAAGAAGCAC
TGATGGAACACATTGATATTCAAGACCTTATTGAACGAGGTCCTCAGAATGCAGAAGAAGAACTTCGTCTTGATATTTTT
AACCGTGTTAATAAATTAGGTATCGGAGCTCAAGGTCTTGGCGGACTTACTACTGTTGTTGATGTAAAAATCAAATCAGC
ACCAACGCACGCAGCCTCTAAACCTGTGTGTTTAATTCCTAACTGTGCTGCTACTCGTCACGTACATTTTACCCTTGATG
GTACAGGTCCTGCTGATCTTCAGCCGCCAAAGTTAGAGGAATGGCCTGATATTACGTGGGAAGCGGGAGCAAATACACGC
CGAGTTAATCTTGATGAGATAACAAAAGAAGACGTTCAAGAGTGGAAAACGGGCGAAACGATCTTGCTTAATGGTAAAAT
TCTAACAGGACGTGACGCGGCACATAAACGTCTTCAAGGAATGATCGAAAGCGGTGAAGGTTTACCTGATGGTGTTGATT
TTAATGGTAAATTCATTTACTACGTAGGCCCTGTTGATGCTGTAGGTGATGAAGCGGTAGGTCCTGCCGGCCCAACGACC
TCTACTCGCATGGATAAATTTACCGATATGATGTTAGCTACAGGCTTAACGGGCATGATAGGTAAAGCGGAACGTGGTCC
AGCAACCGTGGCATCTATTAAAGAAAATAAAGCGGTATATTTAATGGCTGTTGGTGGCGCTGCTTACTTGGTTGCTAAAG
CGATTAAAAAAGCACGTGTTGTTGCCTTTGAAGATCTTGGAATGGAAGCTATTTATGAGTTTGAGGTTGAAGATATGCCA
GTTACTGTGGCCGTTGATTCAACAGGTGTAAATGCGCATCAAATTGGTCCTGATACATGGAAGGTAAAAATTGCTGAAAT
GAGCAATTAA

Upstream 100 bases:

>100_bases
AATGCGAGTTCTAAAAGAAAATAATAGATGGCGATTGCTCACGTCATACACAATAAAAATCATCCTTAATTGCCATGGAA
GCTTATAAAAAGAGGGCCCT

Downstream 100 bases:

>100_bases
TTTACATCATAAAGCTTCTTAAATAATGTAAAAGCATTCATTTTGAGTGCTTTTTTTGTTTTTTGTGTCTTTTATGTTAA
TCATTTGGGTGTAATATATA

Product: anaerobic class I fumarate hydratase (fumarase B)

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MINVHQETLIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREESQAAKDALAQILINSRMSAEGRRPICQDTGIVTC
FVNIGMNVRWEGDLTVQQMIDEGTRQAYTNPDNPLRASVLSDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSE
NKTKMVMLNPSDDIAEWVEKTLPTMGAGWCPPGMLGIGVGGTAEKAAVLAKEALMEHIDIQDLIERGPQNAEEELRLDIF
NRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCLIPNCAATRHVHFTLDGTGPADLQPPKLEEWPDITWEAGANTR
RVNLDEITKEDVQEWKTGETILLNGKILTGRDAAHKRLQGMIESGEGLPDGVDFNGKFIYYVGPVDAVGDEAVGPAGPTT
STRMDKFTDMMLATGLTGMIGKAERGPATVASIKENKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMP
VTVAVDSTGVNAHQIGPDTWKVKIAEMSN

Sequences:

>Translated_509_residues
MINVHQETLIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREESQAAKDALAQILINSRMSAEGRRPICQDTGIVTC
FVNIGMNVRWEGDLTVQQMIDEGTRQAYTNPDNPLRASVLSDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSE
NKTKMVMLNPSDDIAEWVEKTLPTMGAGWCPPGMLGIGVGGTAEKAAVLAKEALMEHIDIQDLIERGPQNAEEELRLDIF
NRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCLIPNCAATRHVHFTLDGTGPADLQPPKLEEWPDITWEAGANTR
RVNLDEITKEDVQEWKTGETILLNGKILTGRDAAHKRLQGMIESGEGLPDGVDFNGKFIYYVGPVDAVGDEAVGPAGPTT
STRMDKFTDMMLATGLTGMIGKAERGPATVASIKENKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMP
VTVAVDSTGVNAHQIGPDTWKVKIAEMSN
>Mature_509_residues
MINVHQETLIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREESQAAKDALAQILINSRMSAEGRRPICQDTGIVTC
FVNIGMNVRWEGDLTVQQMIDEGTRQAYTNPDNPLRASVLSDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSE
NKTKMVMLNPSDDIAEWVEKTLPTMGAGWCPPGMLGIGVGGTAEKAAVLAKEALMEHIDIQDLIERGPQNAEEELRLDIF
NRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCLIPNCAATRHVHFTLDGTGPADLQPPKLEEWPDITWEAGANTR
RVNLDEITKEDVQEWKTGETILLNGKILTGRDAAHKRLQGMIESGEGLPDGVDFNGKFIYYVGPVDAVGDEAVGPAGPTT
STRMDKFTDMMLATGLTGMIGKAERGPATVASIKENKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMP
VTVAVDSTGVNAHQIGPDTWKVKIAEMSN

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=470, Percent_Identity=31.2765957446809, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=30.4255319148936, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789442, Length=276, Percent_Identity=30.7971014492754, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=34.7058823529412, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54980; Mature: 54980

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVHQETLIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREESQAAKDALAQILIN
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMSAEGRRPICQDTGIVTCFVNIGMNVRWEGDLTVQQMIDEGTRQAYTNPDNPLRASVL
CCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEHHHH
SDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEK
CCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHH
TLPTMGAGWCPPGMLGIGVGGTAEKAAVLAKEALMEHIDIQDLIERGPQNAEEELRLDIF
HCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
NRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCLIPNCAATRHVHFTLDGTGPADL
HHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCC
QPPKLEEWPDITWEAGANTRRVNLDEITKEDVQEWKTGETILLNGKILTGRDAAHKRLQG
CCCCCCCCCCCEEECCCCCEECCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHH
MIESGEGLPDGVDFNGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLATGLTGMI
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHC
GKAERGPATVASIKENKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMP
CCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCC
VTVAVDSTGVNAHQIGPDTWKVKIAEMSN
EEEEECCCCCCHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MINVHQETLIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREESQAAKDALAQILIN
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMSAEGRRPICQDTGIVTCFVNIGMNVRWEGDLTVQQMIDEGTRQAYTNPDNPLRASVL
CCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEHHHH
SDPAGKRINTKDNTPAVVHINMVPGNKVEIQIAAKGGGSENKTKMVMLNPSDDIAEWVEK
CCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHH
TLPTMGAGWCPPGMLGIGVGGTAEKAAVLAKEALMEHIDIQDLIERGPQNAEEELRLDIF
HCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
NRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCLIPNCAATRHVHFTLDGTGPADL
HHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCC
QPPKLEEWPDITWEAGANTRRVNLDEITKEDVQEWKTGETILLNGKILTGRDAAHKRLQG
CCCCCCCCCCCEEECCCCCEECCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHH
MIESGEGLPDGVDFNGKFIYYVGPVDAVGDEAVGPAGPTTSTRMDKFTDMMLATGLTGMI
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHC
GKAERGPATVASIKENKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMP
CCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCC
VTVAVDSTGVNAHQIGPDTWKVKIAEMSN
EEEEECCCCCCHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]