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The map label for this gene is sbcD [H]

Identifier: 59711774

GI number: 59711774

Start: 1294503

End: 1295747

Strand: Direct

Name: sbcD [H]

Synonym: VF_1167

Alternate gene names: 59711774

Gene position: 1294503-1295747 (Clockwise)

Preceding gene: 59711772

Following gene: 59711775

Centisome position: 44.68

GC content: 39.84

Gene sequence:

>1245_bases
ATGCGTATTTTACATACTTCTGATTGGCATTTAGGTCAAAATTTCTTCACAAAAAGTCGTCGTAATGAACATCAAAAATT
CATTACCTGGTTACTTGAGCAAGTACAAGAAAATGCCATTAATGCTGTCATTATTGCAGGGGATGTGTTTGATACAGGGG
CGCCACCAAGTTATGCCCGCGAAATGTATAACCAATTTGTGGTGGAGATGAACAAAGTAAATTGTGAGTTGATTGTGCTT
GGTGGAAACCATGACTCAGTTTCAACATTAAATGAATCTAAACAATTATTGGCTCACTTGAACGCACGAGTGATTGCCAA
TACCAATGATGATTTATCCACTCAATTATTAACGCTTCCAGACAATGATGGGGGCGTGGGCGCTATTTTATGTGCGGTTC
CATTTATTCGTCCGCGTGACGTTGTCACTAGCGTGGCGGGTTCAACGGGTGTAGAAAAGCAACAAGCACTCGGTGAAGCG
ATTAAACAGCATTATCATCAGCTGTATCAGAAAGCACTAGAGCTACGAAAAGAATTAAATGTGGAAGCGCCTATTATTGC
AACTGGTCATTTAACCGCACTAGGGGTAAAACAATCGGATTCGGTTCGAGATATTTATATTGGTACGCTTGATGGTTTTG
CTGCGGATGGCTTTCCGCCGGCGGATTACATAGCATTAGGCCATATACACCGACCTCAACTTGTCGCTAAATCTGAACAT
ATTCGCTATTCGGGTTCGCCAATCCCATTAAGTTTTGATGAATTAAAATCTCAAAAACAAGTGGTGATGGTTGAGTTTGA
TAAGCAAGATGTCACTCAAATTACGCCAATTAATGTGCCTATGTTTCAACAAATGAAGGCATTAAAAGGTGATTTGGAAA
ACATCGAAAAAGAATTAGTCCAATTTAAAGAGTGTGAAGAAACAACTTGGTTATGTATTGAAGTTGAAGTGCAAGATTAC
CTTTCCGATCTACAACAACGCATTCAGGCGCTAACGCAAGACCTTAATGTTGAGGTGTTGCAATTGCGTCGTGCAAGAAA
TCGCAGTGAACAAACCTTAGCACAAGAAAAAACAGAAACCTTAGCTGAGTTAACGCCTTTTGATGTGTTTACTAAACGTA
TAGAGCAAGAGACGTTTGAGACAGAAAAAGAGCAGCAACGAGCTGAAAGAATGACTGTGAAATTCAAACAAATTCTGTCA
GAAGTAGAACATAAAGATACAGATCCACAAGAAGGTGATGCATGA

Upstream 100 bases:

>100_bases
GAATAGTTTGATTTTAAAAGCTTATAAAAGTACATAGTTTGTCTTTCAAATGGTATAACTGAATCATAATATTCTTCATC
TAGCGAACAAGTTGATATAT

Downstream 100 bases:

>100_bases
GAATTTTAAGCCTAAGTTTTTCAAATTTAAACTCATTAAAAGGTGAGTGGAAAATTGATTTCTCAGCATCTCCTTTTGCA
GAAAACGGGTTGTTTGCGAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFITWLLEQVQENAINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNDDLSTQLLTLPDNDGGVGAILCAVPFIRPRDVVTSVAGSTGVEKQQALGEA
IKQHYHQLYQKALELRKELNVEAPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKQDVTQITPINVPMFQQMKALKGDLENIEKELVQFKECEETTWLCIEVEVQDY
LSDLQQRIQALTQDLNVEVLQLRRARNRSEQTLAQEKTETLAELTPFDVFTKRIEQETFETEKEQQRAERMTVKFKQILS
EVEHKDTDPQEGDA

Sequences:

>Translated_414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFITWLLEQVQENAINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNDDLSTQLLTLPDNDGGVGAILCAVPFIRPRDVVTSVAGSTGVEKQQALGEA
IKQHYHQLYQKALELRKELNVEAPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKQDVTQITPINVPMFQQMKALKGDLENIEKELVQFKECEETTWLCIEVEVQDY
LSDLQQRIQALTQDLNVEVLQLRRARNRSEQTLAQEKTETLAELTPFDVFTKRIEQETFETEKEQQRAERMTVKFKQILS
EVEHKDTDPQEGDA
>Mature_414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFITWLLEQVQENAINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNDDLSTQLLTLPDNDGGVGAILCAVPFIRPRDVVTSVAGSTGVEKQQALGEA
IKQHYHQLYQKALELRKELNVEAPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKQDVTQITPINVPMFQQMKALKGDLENIEKELVQFKECEETTWLCIEVEVQDY
LSDLQQRIQALTQDLNVEVLQLRRARNRSEQTLAQEKTETLAELTPFDVFTKRIEQETFETEKEQQRAERMTVKFKQILS
EVEHKDTDPQEGDA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=393, Percent_Identity=53.1806615776081, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46879; Mature: 46879

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQNFFTKSRRNEHQKFITWLLEQVQENAINAVIIAGDVFDTGAPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
EMYNQFVVEMNKVNCELIVLGGNHDSVSTLNESKQLLAHLNARVIANTNDDLSTQLLTLP
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECC
DNDGGVGAILCAVPFIRPRDVVTSVAGSTGVEKQQALGEAIKQHYHQLYQKALELRKELN
CCCCCCEEHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEAPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
CCCCEEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHCCCC
IRYSGSPIPLSFDELKSQKQVVMVEFDKQDVTQITPINVPMFQQMKALKGDLENIEKELV
EEECCCCCCCCHHHHCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
QFKECEETTWLCIEVEVQDYLSDLQQRIQALTQDLNVEVLQLRRARNRSEQTLAQEKTET
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHH
LAELTPFDVFTKRIEQETFETEKEQQRAERMTVKFKQILSEVEHKDTDPQEGDA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQNFFTKSRRNEHQKFITWLLEQVQENAINAVIIAGDVFDTGAPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
EMYNQFVVEMNKVNCELIVLGGNHDSVSTLNESKQLLAHLNARVIANTNDDLSTQLLTLP
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECC
DNDGGVGAILCAVPFIRPRDVVTSVAGSTGVEKQQALGEAIKQHYHQLYQKALELRKELN
CCCCCCEEHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEAPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
CCCCEEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHCCCC
IRYSGSPIPLSFDELKSQKQVVMVEFDKQDVTQITPINVPMFQQMKALKGDLENIEKELV
EEECCCCCCCCHHHHCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
QFKECEETTWLCIEVEVQDYLSDLQQRIQALTQDLNVEVLQLRRARNRSEQTLAQEKTET
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHH
LAELTPFDVFTKRIEQETFETEKEQQRAERMTVKFKQILSEVEHKDTDPQEGDA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]