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The map label for this gene is ldhA [H]

Identifier: 59711662

GI number: 59711662

Start: 1164250

End: 1165245

Strand: Reverse

Name: ldhA [H]

Synonym: VF_1055

Alternate gene names: 59711662

Gene position: 1165245-1164250 (Counterclockwise)

Preceding gene: 59711664

Following gene: 59711657

Centisome position: 40.22

GC content: 39.56

Gene sequence:

>996_bases
ATGAAAATCGCAATGTTCAGCACCAAATCTTATGATGAAACATCTTTCAATAGTATCAATCAAAACTATCAGTTTGAATG
TCAGTTTTTCAATTTTCAGTTAACTGAAAGCACGGCTCCTATTGCCAATGGGGCTGATGTTGTCTGTGCATTTGTAAATG
ACGACTTGTCAGCACCAGTGCTAGAAATACTAGCCAAGCAAGGAACAAAACTTATCGCTATGCGATGTGCTGGTTTTGAT
CGTGTTGATCTTGAGGCTGCTAAGAAATTAGGAATGCAAGTAGTTCGTGTTCCTGCTTATTCACCAGAGGCTATTGCCGA
GCATGCCGTTGGTATGATGATGTGTTTAAACCGTCGCTTCCACAAAGCGTATCAACGTACTCGTGATGCTAACTTCTCTC
TAGAAGGCCTTACTGGATTTAACTTTTTCGGTAAAACCGTTGGGGTGATTGGCTCAGGTAAAATTGGTCTAGCAACCATG
CGTATTCTAAAAGGATTGGGAATGAATATATTGTGCTTTGACCCATACCCAAGCCAAGTCGCCATTGATATGGGAGTGAA
ATACACAACTCTTGACGAAATATTTTCTCAATCAGATGTGATCACTCTGCACTGTCCATTAACACCTGAGAATACCCATC
TTCTAGATACAGATTCATTTTCTAAAATGAAAGATGGAGTAATGATCATTAACACAAGCCGTGGTGGATTGCTTAATTCA
GCTAGTGCAATTGAGGCATTAAAAGCTGGGAAAATTGGCTCACTTGGTTTAGATGTTTACGATCACGAAAAAGAGCTTTT
CTTCCAAGACAAATCAAATGACATTATTAAAGATGATGTTTTCCGTCGCTTATCTGCTTGCCACAACGTACTGTTCACAG
GGCATCAAGCATTTCTAACTGAAGAAGCGCTAGACAATATTGCGTCAACAACACTAGGAAATATTGATGCTTTCTTAAAA
GGCGAAGCATCGGGTAATGAACTCATTACCAATTAA

Upstream 100 bases:

>100_bases
AACCTTTTTTATCCCATTCTGTCTTGAGAATTCTTAAGACTAAATGGTAGAGTGGAAACAGTGAAATTAAATATAACCTG
TTTATTTGTAAGGATATTAT

Downstream 100 bases:

>100_bases
CTGACTTAAGCATCTCCTACTCACTGTAGTGAGATGCTTAACTTACTTACCTATCGTAATCTATCTAGCACGACTTGTTG
ATCTAAATGTTCTTGATAAT

Product: fermentative D-lactate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKIAMFSTKSYDETSFNSINQNYQFECQFFNFQLTESTAPIANGADVVCAFVNDDLSAPVLEILAKQGTKLIAMRCAGFD
RVDLEAAKKLGMQVVRVPAYSPEAIAEHAVGMMMCLNRRFHKAYQRTRDANFSLEGLTGFNFFGKTVGVIGSGKIGLATM
RILKGLGMNILCFDPYPSQVAIDMGVKYTTLDEIFSQSDVITLHCPLTPENTHLLDTDSFSKMKDGVMIINTSRGGLLNS
ASAIEALKAGKIGSLGLDVYDHEKELFFQDKSNDIIKDDVFRRLSACHNVLFTGHQAFLTEEALDNIASTTLGNIDAFLK
GEASGNELITN

Sequences:

>Translated_331_residues
MKIAMFSTKSYDETSFNSINQNYQFECQFFNFQLTESTAPIANGADVVCAFVNDDLSAPVLEILAKQGTKLIAMRCAGFD
RVDLEAAKKLGMQVVRVPAYSPEAIAEHAVGMMMCLNRRFHKAYQRTRDANFSLEGLTGFNFFGKTVGVIGSGKIGLATM
RILKGLGMNILCFDPYPSQVAIDMGVKYTTLDEIFSQSDVITLHCPLTPENTHLLDTDSFSKMKDGVMIINTSRGGLLNS
ASAIEALKAGKIGSLGLDVYDHEKELFFQDKSNDIIKDDVFRRLSACHNVLFTGHQAFLTEEALDNIASTTLGNIDAFLK
GEASGNELITN
>Mature_331_residues
MKIAMFSTKSYDETSFNSINQNYQFECQFFNFQLTESTAPIANGADVVCAFVNDDLSAPVLEILAKQGTKLIAMRCAGFD
RVDLEAAKKLGMQVVRVPAYSPEAIAEHAVGMMMCLNRRFHKAYQRTRDANFSLEGLTGFNFFGKTVGVIGSGKIGLATM
RILKGLGMNILCFDPYPSQVAIDMGVKYTTLDEIFSQSDVITLHCPLTPENTHLLDTDSFSKMKDGVMIINTSRGGLLNS
ASAIEALKAGKIGSLGLDVYDHEKELFFQDKSNDIIKDDVFRRLSACHNVLFTGHQAFLTEEALDNIASTTLGNIDAFLK
GEASGNELITN

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=208, Percent_Identity=33.6538461538462, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=32.0987654320988, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=32.0987654320988, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI145580578, Length=243, Percent_Identity=30.0411522633745, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4557499, Length=243, Percent_Identity=30.0411522633745, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI145580575, Length=244, Percent_Identity=30.7377049180328, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI6912396, Length=226, Percent_Identity=31.4159292035398, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=66.5653495440729, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI87082289, Length=282, Percent_Identity=26.241134751773, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789279, Length=248, Percent_Identity=27.8225806451613, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788660, Length=258, Percent_Identity=27.5193798449612, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=257, Percent_Identity=27.6264591439689, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25147481, Length=254, Percent_Identity=29.5275590551181, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=236, Percent_Identity=32.6271186440678, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=236, Percent_Identity=32.2033898305085, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=196, Percent_Identity=32.6530612244898, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=213, Percent_Identity=27.6995305164319, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=141, Percent_Identity=28.3687943262411, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646448, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646452, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646450, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI62472511, Length=268, Percent_Identity=30.5970149253731, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921140, Length=240, Percent_Identity=28.3333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574284, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45551003, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552429, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585514, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574282, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574286, Length=286, Percent_Identity=27.6223776223776, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571528, Length=295, Percent_Identity=26.7796610169492, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585516, Length=231, Percent_Identity=24.6753246753247, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36311; Mature: 36311

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAMFSTKSYDETSFNSINQNYQFECQFFNFQLTESTAPIANGADVVCAFVNDDLSAPV
CEEEEECCCCCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHH
LEILAKQGTKLIAMRCAGFDRVDLEAAKKLGMQVVRVPAYSPEAIAEHAVGMMMCLNRRF
HHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLEGLTGFNFFGKTVGVIGSGKIGLATMRILKGLGMNILCFDPYPSQV
HHHHHHHCCCCCEEECCCCCCHHHHHEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
AIDMGVKYTTLDEIFSQSDVITLHCPLTPENTHLLDTDSFSKMKDGVMIINTSRGGLLNS
EEECCCEEEEHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHCCEEEEECCCCCCCCC
ASAIEALKAGKIGSLGLDVYDHEKELFFQDKSNDIIKDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALDNIASTTLGNIDAFLKGEASGNELITN
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKIAMFSTKSYDETSFNSINQNYQFECQFFNFQLTESTAPIANGADVVCAFVNDDLSAPV
CEEEEECCCCCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHH
LEILAKQGTKLIAMRCAGFDRVDLEAAKKLGMQVVRVPAYSPEAIAEHAVGMMMCLNRRF
HHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLEGLTGFNFFGKTVGVIGSGKIGLATMRILKGLGMNILCFDPYPSQV
HHHHHHHCCCCCEEECCCCCCHHHHHEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
AIDMGVKYTTLDEIFSQSDVITLHCPLTPENTHLLDTDSFSKMKDGVMIINTSRGGLLNS
EEECCCEEEEHHHHHCCCCEEEEECCCCCCCCEEECCCCHHHHHCCEEEEECCCCCCCCC
ASAIEALKAGKIGSLGLDVYDHEKELFFQDKSNDIIKDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALDNIASTTLGNIDAFLKGEASGNELITN
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]