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The map label for this gene is dpnA [H]

Identifier: 59711656

GI number: 59711656

Start: 1156168

End: 1157025

Strand: Reverse

Name: dpnA [H]

Synonym: VF_1049

Alternate gene names: 59711656

Gene position: 1157025-1156168 (Counterclockwise)

Preceding gene: 59711657

Following gene: 172087688

Centisome position: 39.93

GC content: 40.91

Gene sequence:

>858_bases
GTGCTTGTTGAATTTTGGCGAGTACTAAAACCTTCTGGCAACTTATATCTATTTTGCGGTTCAAAGTTAACAGCGGATAC
TGAGTTGCTTATTCGTGCGCGATTTAACGTATTTAATCACATTTTTTGGGCGAAACCTTCAGGGGTGTGGAAACGCGCGC
ACAAACCGCACTTACGGGCGTTCTTTCCTGCGACTGAACGTATTATCTTTGCGGGACATTATGACTCTGAAGGATACGCG
AAAGGATGTAGCCAGTACGCAACCAAATGCAATGAACTAAAAAAAACCACCTTTAAACCACTGATGGATTATTTCAAAAA
CACGAAGGACGCACTCAATATCTCAGCAAAAGAAATCAATGAGGCCACTGGCACACAAATGTGCTCACACTGGTTTTCAT
CGAGTCAATGGAAACTGCCCACAGAGAAACAATACCAACAACTGCAAACCTTGTTCGCATCAAAAAGTGGTCAATTACCC
AAATCACACTCCGACCTTACAACTGAGTACCAAGCATTGCACAATGAATACGGAAAACTGATCAAGGAATACGATGAACT
AAAAGCGGAATATGAAAACCTCAGAAGGCCTTTTCATGTCACAGCCGAAGTACCTTATACCGACGTATGGACATTTGCAC
CAGTTCAATATTATCCAGGCAAACACCCTTGTGAAAAACCTGCAAATTTACTCGAGCATATTATTGCAAGTAGTAGTCGA
GAAGGTGCAGTGGTACTTGATGCATTTATGGGATCTGGTTCAACAGGAAAAGCGTGCTTAGCACTTAATAGAAAGTTTGT
GGGGATTGAGATGGAAGAAGAAATGTATACAAAAACAATTAATACATTTAAAGGTTAA

Upstream 100 bases:

>100_bases
ACTCTTTAAAACTTGATAGAATTCTTTATAACACTGCTTAAATCACGCCTGGGATAATCAATGGCCAGATGTAGAAACCT
TCCTGGCATGGCTTGATGAA

Downstream 100 bases:

>100_bases
TATTAAATGAAAAAACACTCGGCAGACTAATATGCCGAGTGTAGCAAATCGTTATTGATGCATTTGTTATATATCTTTTA
CACATATTTTCTTATAAGCT

Product: putative DNA methylase

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MLVEFWRVLKPSGNLYLFCGSKLTADTELLIRARFNVFNHIFWAKPSGVWKRAHKPHLRAFFPATERIIFAGHYDSEGYA
KGCSQYATKCNELKKTTFKPLMDYFKNTKDALNISAKEINEATGTQMCSHWFSSSQWKLPTEKQYQQLQTLFASKSGQLP
KSHSDLTTEYQALHNEYGKLIKEYDELKAEYENLRRPFHVTAEVPYTDVWTFAPVQYYPGKHPCEKPANLLEHIIASSSR
EGAVVLDAFMGSGSTGKACLALNRKFVGIEMEEEMYTKTINTFKG

Sequences:

>Translated_285_residues
MLVEFWRVLKPSGNLYLFCGSKLTADTELLIRARFNVFNHIFWAKPSGVWKRAHKPHLRAFFPATERIIFAGHYDSEGYA
KGCSQYATKCNELKKTTFKPLMDYFKNTKDALNISAKEINEATGTQMCSHWFSSSQWKLPTEKQYQQLQTLFASKSGQLP
KSHSDLTTEYQALHNEYGKLIKEYDELKAEYENLRRPFHVTAEVPYTDVWTFAPVQYYPGKHPCEKPANLLEHIIASSSR
EGAVVLDAFMGSGSTGKACLALNRKFVGIEMEEEMYTKTINTFKG
>Mature_285_residues
MLVEFWRVLKPSGNLYLFCGSKLTADTELLIRARFNVFNHIFWAKPSGVWKRAHKPHLRAFFPATERIIFAGHYDSEGYA
KGCSQYATKCNELKKTTFKPLMDYFKNTKDALNISAKEINEATGTQMCSHWFSSSQWKLPTEKQYQQLQTLFASKSGQLP
KSHSDLTTEYQALHNEYGKLIKEYDELKAEYENLRRPFHVTAEVPYTDVWTFAPVQYYPGKHPCEKPANLLEHIIASSSR
EGAVVLDAFMGSGSTGKACLALNRKFVGIEMEEEMYTKTINTFKG

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=70, Percent_Identity=45.7142857142857, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32657; Mature: 32657

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVEFWRVLKPSGNLYLFCGSKLTADTELLIRARFNVFNHIFWAKPSGVWKRAHKPHLRA
CHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
FFPATERIIFAGHYDSEGYAKGCSQYATKCNELKKTTFKPLMDYFKNTKDALNISAKEIN
ECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
EATGTQMCSHWFSSSQWKLPTEKQYQQLQTLFASKSGQLPKSHSDLTTEYQALHNEYGKL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IKEYDELKAEYENLRRPFHVTAEVPYTDVWTFAPVQYYPGKHPCEKPANLLEHIIASSSR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCC
EGAVVLDAFMGSGSTGKACLALNRKFVGIEMEEEMYTKTINTFKG
CCCEEEEEECCCCCCCCEEEEECCEEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVEFWRVLKPSGNLYLFCGSKLTADTELLIRARFNVFNHIFWAKPSGVWKRAHKPHLRA
CHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
FFPATERIIFAGHYDSEGYAKGCSQYATKCNELKKTTFKPLMDYFKNTKDALNISAKEIN
ECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
EATGTQMCSHWFSSSQWKLPTEKQYQQLQTLFASKSGQLPKSHSDLTTEYQALHNEYGKL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IKEYDELKAEYENLRRPFHVTAEVPYTDVWTFAPVQYYPGKHPCEKPANLLEHIIASSSR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCC
EGAVVLDAFMGSGSTGKACLALNRKFVGIEMEEEMYTKTINTFKG
CCCEEEEEECCCCCCCCEEEEECCEEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782 [H]