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The map label for this gene is mifA [H]

Identifier: 59711596

GI number: 59711596

Start: 1089083

End: 1090978

Strand: Direct

Name: mifA [H]

Synonym: VF_0989

Alternate gene names: 59711596

Gene position: 1089083-1090978 (Clockwise)

Preceding gene: 59711595

Following gene: 59711617

Centisome position: 37.59

GC content: 33.49

Gene sequence:

>1896_bases
ATGAATCTCAAGCAAATAAAATATTTTATCGTTACATTTATATTAATCGGAGCTACTCCGATTTTTTATTTTTGGACACT
ATATCAAACAGAGCTGAACGCCATTCAATCTTTAAGAGAGACTGAGGCTAAGCATCAGCTAAATTATAGTCAAAATGCCA
TCAATGAAATCTCTTCAGAGTTATCTACTTCATTACGATTATTGTCAGACAGTCGTGCATTATTGCAGTTTATTCAATCA
CCATCTTTAAAAAATAGAGCATTACTTGAATCTCTATGGGCACTAACTGCAACTAACTATTCATATATCTCACAAGTGCG
TTTTATTGATACCAATGGTATAGAGCAATCTCGGGTTGATGATATTAATGGTGAAATAAAAGTTACCCCTATTTACCAAT
TACAAGATAAGTCTAAGCGTAACTATTTTCTATATGCACAAACATTAAAGGCGGAAGAGGTTGGTACTTTTGGTATTGAT
CTTGAAGTAGAAAATGGAAAAGTAGTTATACCTCTTCAACCAACGTTACGTATTTTTACCCCTGTAGAAGTGGCTGATGA
GCGAAAAGGGTATTTGGTTTTTAATTTAGATGTTTATGAAATAATTGAAGAGATTGATTATTCGATCGATAGTCATTATA
ACCTAGAATTTGTTGACCTAGAGGGGTATTACCTAGCATCAAAAAATAAAGAAAAGATGTTAGGTAACCTTATAGAAGAA
AGAGATAGTTTTAATTTATTGATTGAAAATCCTAATTTATGGAGAGCGATGGAAGATCGTAACATCCATAATTTCAAAGA
TAAGAAACATTTTTATGCATTTAATAAAATAGCATTAGGTAATGGATTTGAAGGAAGCGAACTTTATTTACTATTAACAA
GTAATATTAAAGTAGACTCTTCAGGTACAGATCATAAATTGGCATTTATCCTTTATGAAACAATAATGGCTATTATCTTA
CTTGTTATCTTATCCGTTATTATTTCTCGATACATCGTAAAACATCGAACAACTAGCCTTGAAAGTCAATTAGCTTTAGC
TGCCTTGAATGGTATGTCTTCGATTGTGATCACAGATAAGAACAACCGCATCATCAAGGTAAATGACGAGTTTACTCGAG
TTAGTGGGTGGAAAGAATCTGAAGTGATCGGGCAATGTCCTTCAATCTTTCAATCTGGTGTTCATAGTGATGCATTTTAT
GCTGAAATGTGGCAAACAATTCAAGAAAATCAGAGTTGGGAAGGTGAAGTTGTTAATAAAAGAAAAGATGGAACCTTAAT
TACTGAGATATTACGAATTCAAGCGATCATGGGTATAAATGGTAAGGCTCAATACTTTATTGCTTCATTTGTTGATATTA
CTGCTCGAAAAGAATTAGAAAACTGCTTAAGAGAGTTGAGTGAAAAAGACATGTTGACTCAGTGCTGGAATAGAAGAAAG
TTTGAAGCTGAGTTTTCAAAATTAACAAATAAGCAGCGTTTAACGCCTGAAGTAAAAATGTCTTGTTTGGCTATCATTGA
TATCGATTATTTTAAACGTATTAATGATCAATACGGACATGATGTCGGCGACAAAGTTATTAAAAATGTCGCTAGAATTT
TGCAAACAGAAAGTCGAAGTATTGATTTTGTAGCAAGAATTGGCGGAGAAGAGTTTGCTATTTTACTTCCAAATACAAGT
TTGAAAGAAGCTGAAGTGATTTTAAATCGCTTAAGGGTTGCTATTCATCTTGCTGATGGTTCACAATCAGTAACCGTTAG
TGGCGGTGTAACTGATATTTGTGATTGTGGTGAGGCAACTTACAAACGTGCCGATCTTGCCTTATATGAATCAAAAACGG
CCGGTCGAAATCAAATATCTTGTTTTCCATCCAGTGAAATGGATCAAATCGCATGA

Upstream 100 bases:

>100_bases
TTAATTGAGCTATTCATCTAAACTATTAGAGTGCGATCACTAATTTATTTTCTATATTTGATAAAATACCCATGAAAAAA
TTAGTTATTTATTAGGTTAC

Downstream 100 bases:

>100_bases
TTATTTTTTTGAAAAAATAGATGTTACTTTCTTCATTATAGGAGTGTGATTGTCTATTTGATGATTTTTCATTTCCTTAA
AGATATTTAATAGAGCTTTA

Product: diguanylate cyclase (GGDEF), inhibitor of flagellar motility

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MNLKQIKYFIVTFILIGATPIFYFWTLYQTELNAIQSLRETEAKHQLNYSQNAINEISSELSTSLRLLSDSRALLQFIQS
PSLKNRALLESLWALTATNYSYISQVRFIDTNGIEQSRVDDINGEIKVTPIYQLQDKSKRNYFLYAQTLKAEEVGTFGID
LEVENGKVVIPLQPTLRIFTPVEVADERKGYLVFNLDVYEIIEEIDYSIDSHYNLEFVDLEGYYLASKNKEKMLGNLIEE
RDSFNLLIENPNLWRAMEDRNIHNFKDKKHFYAFNKIALGNGFEGSELYLLLTSNIKVDSSGTDHKLAFILYETIMAIIL
LVILSVIISRYIVKHRTTSLESQLALAALNGMSSIVITDKNNRIIKVNDEFTRVSGWKESEVIGQCPSIFQSGVHSDAFY
AEMWQTIQENQSWEGEVVNKRKDGTLITEILRIQAIMGINGKAQYFIASFVDITARKELENCLRELSEKDMLTQCWNRRK
FEAEFSKLTNKQRLTPEVKMSCLAIIDIDYFKRINDQYGHDVGDKVIKNVARILQTESRSIDFVARIGGEEFAILLPNTS
LKEAEVILNRLRVAIHLADGSQSVTVSGGVTDICDCGEATYKRADLALYESKTAGRNQISCFPSSEMDQIA

Sequences:

>Translated_631_residues
MNLKQIKYFIVTFILIGATPIFYFWTLYQTELNAIQSLRETEAKHQLNYSQNAINEISSELSTSLRLLSDSRALLQFIQS
PSLKNRALLESLWALTATNYSYISQVRFIDTNGIEQSRVDDINGEIKVTPIYQLQDKSKRNYFLYAQTLKAEEVGTFGID
LEVENGKVVIPLQPTLRIFTPVEVADERKGYLVFNLDVYEIIEEIDYSIDSHYNLEFVDLEGYYLASKNKEKMLGNLIEE
RDSFNLLIENPNLWRAMEDRNIHNFKDKKHFYAFNKIALGNGFEGSELYLLLTSNIKVDSSGTDHKLAFILYETIMAIIL
LVILSVIISRYIVKHRTTSLESQLALAALNGMSSIVITDKNNRIIKVNDEFTRVSGWKESEVIGQCPSIFQSGVHSDAFY
AEMWQTIQENQSWEGEVVNKRKDGTLITEILRIQAIMGINGKAQYFIASFVDITARKELENCLRELSEKDMLTQCWNRRK
FEAEFSKLTNKQRLTPEVKMSCLAIIDIDYFKRINDQYGHDVGDKVIKNVARILQTESRSIDFVARIGGEEFAILLPNTS
LKEAEVILNRLRVAIHLADGSQSVTVSGGVTDICDCGEATYKRADLALYESKTAGRNQISCFPSSEMDQIA
>Mature_631_residues
MNLKQIKYFIVTFILIGATPIFYFWTLYQTELNAIQSLRETEAKHQLNYSQNAINEISSELSTSLRLLSDSRALLQFIQS
PSLKNRALLESLWALTATNYSYISQVRFIDTNGIEQSRVDDINGEIKVTPIYQLQDKSKRNYFLYAQTLKAEEVGTFGID
LEVENGKVVIPLQPTLRIFTPVEVADERKGYLVFNLDVYEIIEEIDYSIDSHYNLEFVDLEGYYLASKNKEKMLGNLIEE
RDSFNLLIENPNLWRAMEDRNIHNFKDKKHFYAFNKIALGNGFEGSELYLLLTSNIKVDSSGTDHKLAFILYETIMAIIL
LVILSVIISRYIVKHRTTSLESQLALAALNGMSSIVITDKNNRIIKVNDEFTRVSGWKESEVIGQCPSIFQSGVHSDAFY
AEMWQTIQENQSWEGEVVNKRKDGTLITEILRIQAIMGINGKAQYFIASFVDITARKELENCLRELSEKDMLTQCWNRRK
FEAEFSKLTNKQRLTPEVKMSCLAIIDIDYFKRINDQYGHDVGDKVIKNVARILQTESRSIDFVARIGGEEFAILLPNTS
LKEAEVILNRLRVAIHLADGSQSVTVSGGVTDICDCGEATYKRADLALYESKTAGRNQISCFPSSEMDQIA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=35.5421686746988, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=38.953488372093, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788085, Length=194, Percent_Identity=32.4742268041237, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788381, Length=282, Percent_Identity=25.886524822695, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=36.4640883977901, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=32.5581395348837, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=286, Percent_Identity=28.3216783216783, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693134, Length=182, Percent_Identity=34.6153846153846, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=35.5029585798817, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787056, Length=155, Percent_Identity=36.1290322580645, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=33.7423312883436, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 72020; Mature: 72020

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQIKYFIVTFILIGATPIFYFWTLYQTELNAIQSLRETEAKHQLNYSQNAINEISSE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSTSLRLLSDSRALLQFIQSPSLKNRALLESLWALTATNYSYISQVRFIDTNGIEQSRVD
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCCCC
DINGEIKVTPIYQLQDKSKRNYFLYAQTLKAEEVGTFGIDLEVENGKVVIPLQPTLRIFT
CCCCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEE
PVEVADERKGYLVFNLDVYEIIEEIDYSIDSHYNLEFVDLEGYYLASKNKEKMLGNLIEE
CHHHCCCCCCEEEEECHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHC
RDSFNLLIENPNLWRAMEDRNIHNFKDKKHFYAFNKIALGNGFEGSELYLLLTSNIKVDS
CCCCEEEEECCCCEEHHCCCCCCCCCCCHHEEEEEEEEECCCCCCCEEEEEEECCEEECC
SGTDHKLAFILYETIMAIILLVILSVIISRYIVKHRTTSLESQLALAALNGMSSIVITDK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
NNRIIKVNDEFTRVSGWKESEVIGQCPSIFQSGVHSDAFYAEMWQTIQENQSWEGEVVNK
CCEEEEECCCHHCCCCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
RKDGTLITEILRIQAIMGINGKAQYFIASFVDITARKELENCLRELSEKDMLTQCWNRRK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FEAEFSKLTNKQRLTPEVKMSCLAIIDIDYFKRINDQYGHDVGDKVIKNVARILQTESRS
HHHHHHHHCCCCCCCHHHHEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
IDFVARIGGEEFAILLPNTSLKEAEVILNRLRVAIHLADGSQSVTVSGGVTDICDCGEAT
EEEEEECCCCEEEEEECCCCCHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHCCCCHH
YKRADLALYESKTAGRNQISCFPSSEMDQIA
HHHHHEEEEECCCCCCCEEEECCCCCHHHCC
>Mature Secondary Structure
MNLKQIKYFIVTFILIGATPIFYFWTLYQTELNAIQSLRETEAKHQLNYSQNAINEISSE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSTSLRLLSDSRALLQFIQSPSLKNRALLESLWALTATNYSYISQVRFIDTNGIEQSRVD
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCCCC
DINGEIKVTPIYQLQDKSKRNYFLYAQTLKAEEVGTFGIDLEVENGKVVIPLQPTLRIFT
CCCCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEE
PVEVADERKGYLVFNLDVYEIIEEIDYSIDSHYNLEFVDLEGYYLASKNKEKMLGNLIEE
CHHHCCCCCCEEEEECHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHC
RDSFNLLIENPNLWRAMEDRNIHNFKDKKHFYAFNKIALGNGFEGSELYLLLTSNIKVDS
CCCCEEEEECCCCEEHHCCCCCCCCCCCHHEEEEEEEEECCCCCCCEEEEEEECCEEECC
SGTDHKLAFILYETIMAIILLVILSVIISRYIVKHRTTSLESQLALAALNGMSSIVITDK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
NNRIIKVNDEFTRVSGWKESEVIGQCPSIFQSGVHSDAFYAEMWQTIQENQSWEGEVVNK
CCEEEEECCCHHCCCCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
RKDGTLITEILRIQAIMGINGKAQYFIASFVDITARKELENCLRELSEKDMLTQCWNRRK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FEAEFSKLTNKQRLTPEVKMSCLAIIDIDYFKRINDQYGHDVGDKVIKNVARILQTESRS
HHHHHHHHCCCCCCCHHHHEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
IDFVARIGGEEFAILLPNTSLKEAEVILNRLRVAIHLADGSQSVTVSGGVTDICDCGEAT
EEEEEECCCCEEEEEECCCCCHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHCCCCHH
YKRADLALYESKTAGRNQISCFPSSEMDQIA
HHHHHEEEEECCCCCCCEEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA