Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 59711569

GI number: 59711569

Start: 1054909

End: 1056261

Strand: Direct

Name: tolB [H]

Synonym: VF_0962

Alternate gene names: 59711569

Gene position: 1054909-1056261 (Clockwise)

Preceding gene: 172087680

Following gene: 59711570

Centisome position: 36.41

GC content: 38.58

Gene sequence:

>1353_bases
GTGCTTAAACGATGGATATTAACCCTATTTGTGGTTTGTTTAGGTTTTAGTCAGGTTGCAAAAGCCGAGCTAGAACTGGT
TATTACAGAAGGTATTGATTCAGCAAGACCAATTGGTATTGTGCCTTTTAAATGGCATGGAGAGGGCAAGTTACCGCAAG
ATATATCTGCGATTATTTCGTCTGATTTACAGCGTAGTGGTAAGTTTAGTCCTGTTGCAACTAATAAAATGCCACAAACA
CCTTATAAAGATTCGGATATTAATTACGAAGCTTGGACAAAAATGGGTGTGGATGCGATTGTTACTGGCGAAGTTAAATT
AAACGCACAAGGTAAGTATGAAGTTACATACAAACTTATCGATGTGGTTCGTGGTCAGTTAACTAAAGGTCAATCTAAGG
CATTAGATGAATCAGGTAAGTTAGTACTTACTCGAGACCATATTTTAGTTAATAAAGTGGCGTCATTATCGACAAAGCAA
TTGCGTAAATATGCACACCGTATTTCTGATGTGGTGTATGAGAAATTGACAGGTGAAAGAGGTGCGTTTTTAACTCGTAT
CGCTTATGTGGTTGTAAATGACAAAGCACAATACCCATACCAGTTAAGAATTGCTGACTATGATGGTTATAATGAACGCT
TAGTTCTGAAATCGAAACAACCTATCATGTCACCAGCATGGTCACCTGATGGTAAAAAGCTTGCATATGTAAGTTTTGAA
AATAGAAAGTCACAAATTTTCATTATGAATATTTATACGGGTAAGAGAGAGTTAATTGCTTCTTATCCGCGTCATAATGG
AGCACCGCGCTTCTCACATGATGGTGATAAGTTAGCGATAGTATTATCAAAATCTGGAAGTCTACAAATTTATGTTGTGG
ATTTGAAGACCAAAAAAATGAGTCAAATTACTCGCGGTAGAGCGAATAATACCGAACCTTTTTGGGCTCCAGATAACAAG
TCACTTATATTTACATCAGATCGGGGTGGTAAACCTCAGATATATAGAGTAAATTTAGGGGATGGTTCGACTAAACGTTT
AACCTGGCAAGGCAGTCAGAATCTTGGTGGGCAGATTACTCCAGACGGTAAATACATCGTTATGGTAAATCGCTCAGAGA
CTGGATTTAATCTAGCGAAACAAGATTTAGAAACCGGTGCTGTCCAGGTCTTAACAAAGACACTACTGGATGAGTCACCA
AGTATTGCGCCAAATGGTGGCATGGTGATTTACAGTTCAATCTATAGAAAACAAAATGTTCTTTCTATGGTTTCTATAGA
TGGCCGTTTTAAAGCTCGCTTACCAGCGACTAACGGTCGTGTAAGGGCACCAGCGTGGTCGCCATTTTTATAG

Upstream 100 bases:

>100_bases
GTAACAAAAGTGAATGTGTTTCCTATGCCAAAGGATGCAGATGTTGTTGCTAAGTTAACGAATATAAACCTTACCGTTCA
ACCAGAATAAGGAAGAGCAA

Downstream 100 bases:

>100_bases
CTACTAAAAATATTTAGGAAAATAAGGAAAATACAATGCAACTAAATAAACTTCTTAAGAGCCTTTTAATCGCGTTGCCT
GTGGTTGCTCTTTCAGCTTG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLKRWILTLFVVCLGFSQVAKAELELVITEGIDSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPVATNKMPQT
PYKDSDINYEAWTKMGVDAIVTGEVKLNAQGKYEVTYKLIDVVRGQLTKGQSKALDESGKLVLTRDHILVNKVASLSTKQ
LRKYAHRISDVVYEKLTGERGAFLTRIAYVVVNDKAQYPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRKSQIFIMNIYTGKRELIASYPRHNGAPRFSHDGDKLAIVLSKSGSLQIYVVDLKTKKMSQITRGRANNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLGDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Sequences:

>Translated_450_residues
MLKRWILTLFVVCLGFSQVAKAELELVITEGIDSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPVATNKMPQT
PYKDSDINYEAWTKMGVDAIVTGEVKLNAQGKYEVTYKLIDVVRGQLTKGQSKALDESGKLVLTRDHILVNKVASLSTKQ
LRKYAHRISDVVYEKLTGERGAFLTRIAYVVVNDKAQYPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRKSQIFIMNIYTGKRELIASYPRHNGAPRFSHDGDKLAIVLSKSGSLQIYVVDLKTKKMSQITRGRANNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLGDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL
>Mature_450_residues
MLKRWILTLFVVCLGFSQVAKAELELVITEGIDSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPVATNKMPQT
PYKDSDINYEAWTKMGVDAIVTGEVKLNAQGKYEVTYKLIDVVRGQLTKGQSKALDESGKLVLTRDHILVNKVASLSTKQ
LRKYAHRISDVVYEKLTGERGAFLTRIAYVVVNDKAQYPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRKSQIFIMNIYTGKRELIASYPRHNGAPRFSHDGDKLAIVLSKSGSLQIYVVDLKTKKMSQITRGRANNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLGDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=449, Percent_Identity=49.6659242761693, Blast_Score=467, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 50217; Mature: 50217

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRWILTLFVVCLGFSQVAKAELELVITEGIDSARPIGIVPFKWHGEGKLPQDISAIIS
CHHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPVATNKMPQTPYKDSDINYEAWTKMGVDAIVTGEVKLNAQGKYEVTYKLI
HHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEHHHHH
DVVRGQLTKGQSKALDESGKLVLTRDHILVNKVASLSTKQLRKYAHRISDVVYEKLTGER
HHHHHHCCCCCHHHCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKAQYPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NRKSQIFIMNIYTGKRELIASYPRHNGAPRFSHDGDKLAIVLSKSGSLQIYVVDLKTKKM
CCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCHHH
SQITRGRANNTEPFWAPDNKSLIFTSDRGGKPQIYRVNLGDGSTKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEC
PDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYRKQNV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCC
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MLKRWILTLFVVCLGFSQVAKAELELVITEGIDSARPIGIVPFKWHGEGKLPQDISAIIS
CHHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPVATNKMPQTPYKDSDINYEAWTKMGVDAIVTGEVKLNAQGKYEVTYKLI
HHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEHHHHH
DVVRGQLTKGQSKALDESGKLVLTRDHILVNKVASLSTKQLRKYAHRISDVVYEKLTGER
HHHHHHCCCCCHHHCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKAQYPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NRKSQIFIMNIYTGKRELIASYPRHNGAPRFSHDGDKLAIVLSKSGSLQIYVVDLKTKKM
CCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCHHH
SQITRGRANNTEPFWAPDNKSLIFTSDRGGKPQIYRVNLGDGSTKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEC
PDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYRKQNV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCC
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA