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The map label for this gene is aspS [H]

Identifier: 59711556

GI number: 59711556

Start: 1044375

End: 1046150

Strand: Direct

Name: aspS [H]

Synonym: VF_0949

Alternate gene names: 59711556

Gene position: 1044375-1046150 (Clockwise)

Preceding gene: 59711550

Following gene: 59711557

Centisome position: 36.04

GC content: 42.91

Gene sequence:

>1776_bases
ATGCGCACCCATTACTGTGGTAACCTGAACAAGTCCCTTGCAGGGCAAACTGTAGAACTTTGCGGCTGGGTAAACCGTCG
CCGTGATTTAGGCGGTCTTATCTTTATTGATATGCGAGATCGTGAAGGTATCGTTCAGGTAGTTGTTGACCCAGATATGA
AAGACGTATTTGAGGTTGCAAGTCAACTGCGTAATGAATTCTGTATCAAATTTACTGGTGAAGTTCGTGTTCGTCCTGAT
AGCCAAGTAAATAAAGATATGGCTACTGGTGAAGTAGAACTTTTGGCTACAGGCCTTGAGATCATCAACCGTTCTGCGGC
GCTTCCATTAGACTTCAACCAAACTAACTCAGAAGAGCAACGTCTGAAGTATCGTTACATTGATTTACGTCGTCCAGAAA
TGAGTGATCGTATCAAGTTACGTGCTCGTGCATCAAGCTTCGTTCGTCGTTTCTTAGATGAAAACTTATTCTTAGATATC
GAAACGCCAGTATTAACGAAAGCAACACCAGAAGGTGCTCGTGATTACTTAGTACCAAGCCGTGTTCATAAAGGTAGCTT
CTACGCTCTACCACAATCACCACAGCTATTTAAACAATTGCTAATGATGTCTGGTTTTGACCGTTACTACCAAATCGTAA
AATGTTTCCGTGATGAAGATTTACGTGCTGACCGTCAACCTGAATTTACTCAAATCGATATCGAAACTTCATTCATGACT
TCTCAAGAAGTTCGTAATGTAACTGAGCGTCTTGTTCACGATATGTGGAAAGAGCTTTTAGATGTTGAGCTTGGTCAATT
CCCTGTAATGCCATTCTCTGAAGCGATGCGTCGTTTTGGTTCTGATAAACCAGATCTACGTAACCCACTTGAACTTGTTG
ACGTTGCTGATCTTGTTAAAGATGTAGACTTCAAAGTATTCTCAGGTCCTGCAAATGATGAAAAAGGTCGTGTAGCAGTT
ATCCGTGTACCTGGTGGTGCTTCATTATCTCGTAAGCAAATCGATGAGTACGGTAACTTCGTAGGTATCTACGGTGCGAA
AGGCTTAGCTTGGATGAAAGTGAACGATCGTGCTGCAGGTTTTGAAGGTGTTCAATCTCCAGTTGCTAAGTTCTTAAATG
AAGAAGTAGTAAACGCAATTCTTGAGCGTACTCAAGCTGAAACAGGCGACATCATCCTATTTGGTGCCGATAAAGCAGGT
ATCGTATCGGAAGCTATGGGTGCACTACGTCTTAAGTTAGGTACTGACCTTGAGCTAACAGATACATCTGCATGGGCTCC
GTTATGGGTTGTTGATTTCCCAATGTTTGAAGAAGATGGTGAAGGTAACTTGCATGCGATGCACCACCCATTCACATCTC
CTTTAGGTGTTTCTGCTGAAGAGCTACAAGCTAACCCAGCTGTAGCGAACTCTGATGCATACGATATGGTAATTAACGGC
TACGAAGTTGGTGGTGGTTCTGTACGTATCCATAATGCAGAAATGCAAACAGCGGTATTTGGTATCCTAGGTATCGAAGC
TAAAGAACAACAAGAGAAATTTGGTTTCTTACTTGAAGCTCTAAAATACGGCACGCCGCCACACGCAGGTCTTGCTTTTG
GTCTTGACCGTCTAGCAATGCTACTTTGTGGTACAGAAAACATCCGTGATGTTATCGCGTTCCCTAAAACAACGGCTGCA
GCATGTCTACTTACAGACGCTCCAAGCCTAGCAAACCCTGCTTCTTTAGAAGAATTGGCGATTGCTGTTAAACTTGCAGA
GAAAAAAGACGCATAA

Upstream 100 bases:

>100_bases
TACGTCATTTAGGCAAGCGTGAATAGAATTATGAAAAATGCAGCACTCCTTTTTTGCTGCATTTACTATTGAACATGTAT
ATTAGATTGGGAAAATCATC

Downstream 100 bases:

>100_bases
TTAGCGTTTAACTCTTTGTAAAAAATGCCCGTATTTATGCGGGCATTTTTGTATCTATACTATGTAAAGTGAGACAGCTC
TTTATTACTAATACCTTGAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRTHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMKDVFEVASQLRNEFCIKFTGEVRVRPD
SQVNKDMATGEVELLATGLEIINRSAALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
SQEVRNVTERLVHDMWKELLDVELGQFPVMPFSEAMRRFGSDKPDLRNPLELVDVADLVKDVDFKVFSGPANDEKGRVAV
IRVPGGASLSRKQIDEYGNFVGIYGAKGLAWMKVNDRAAGFEGVQSPVAKFLNEEVVNAILERTQAETGDIILFGADKAG
IVSEAMGALRLKLGTDLELTDTSAWAPLWVVDFPMFEEDGEGNLHAMHHPFTSPLGVSAEELQANPAVANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTDAPSLANPASLEELAIAVKLAEKKDA

Sequences:

>Translated_591_residues
MRTHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMKDVFEVASQLRNEFCIKFTGEVRVRPD
SQVNKDMATGEVELLATGLEIINRSAALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
SQEVRNVTERLVHDMWKELLDVELGQFPVMPFSEAMRRFGSDKPDLRNPLELVDVADLVKDVDFKVFSGPANDEKGRVAV
IRVPGGASLSRKQIDEYGNFVGIYGAKGLAWMKVNDRAAGFEGVQSPVAKFLNEEVVNAILERTQAETGDIILFGADKAG
IVSEAMGALRLKLGTDLELTDTSAWAPLWVVDFPMFEEDGEGNLHAMHHPFTSPLGVSAEELQANPAVANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTDAPSLANPASLEELAIAVKLAEKKDA
>Mature_591_residues
MRTHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMKDVFEVASQLRNEFCIKFTGEVRVRPD
SQVNKDMATGEVELLATGLEIINRSAALPLDFNQTNSEEQRLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
SQEVRNVTERLVHDMWKELLDVELGQFPVMPFSEAMRRFGSDKPDLRNPLELVDVADLVKDVDFKVFSGPANDEKGRVAV
IRVPGGASLSRKQIDEYGNFVGIYGAKGLAWMKVNDRAAGFEGVQSPVAKFLNEEVVNAILERTQAETGDIILFGADKAG
IVSEAMGALRLKLGTDLELTDTSAWAPLWVVDFPMFEEDGEGNLHAMHHPFTSPLGVSAEELQANPAVANSDAYDMVING
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAMLLCGTENIRDVIAFPKTTAA
ACLLTDAPSLANPASLEELAIAVKLAEKKDA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=596, Percent_Identity=38.5906040268456, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=255, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI194272210, Length=255, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI45439306, Length=298, Percent_Identity=26.1744966442953, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788173, Length=583, Percent_Identity=73.9279588336192, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=24.7557003257329, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790571, Length=288, Percent_Identity=24.3055555555556, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=612, Percent_Identity=34.9673202614379, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=261, Percent_Identity=23.3716475095785, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=261, Percent_Identity=23.3716475095785, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=261, Percent_Identity=23.3716475095785, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=297, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=638, Percent_Identity=34.1692789968652, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=27.2, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=24.2307692307692, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323011, Length=279, Percent_Identity=27.2401433691756, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=35.4892205638474, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=28.0155642023346, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136276, Length=276, Percent_Identity=26.4492753623188, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65829; Mature: 65829

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMKDVFEVA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
SQLRNEFCIKFTGEVRVRPDSQVNKDMATGEVELLATGLEIINRSAALPLDFNQTNSEEQ
HHHCCCEEEEEECCEEECCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDIETPVLTKATPEGARDYLVPS
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
SQEVRNVTERLVHDMWKELLDVELGQFPVMPFSEAMRRFGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVDFKVFSGPANDEKGRVAVIRVPGGASLSRKQIDEYGNFVGIYGAKGLAWMKVNDRAAG
HCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEECCCCEEEEEECCCCCC
FEGVQSPVAKFLNEEVVNAILERTQAETGDIILFGADKAGIVSEAMGALRLKLGTDLELT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHEEEECCCCEEEC
DTSAWAPLWVVDFPMFEEDGEGNLHAMHHPFTSPLGVSAEELQANPAVANSDAYDMVING
CCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEE
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAM
EEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLLTDAPSLANPASLEELAIAVKLAEKKDA
HHHCCCCHHHHHCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHEECCCCCC
>Mature Secondary Structure
MRTHYCGNLNKSLAGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMKDVFEVA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
SQLRNEFCIKFTGEVRVRPDSQVNKDMATGEVELLATGLEIINRSAALPLDFNQTNSEEQ
HHHCCCEEEEEECCEEECCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYIDLRRPEMSDRIKLRARASSFVRRFLDENLFLDIETPVLTKATPEGARDYLVPS
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
SQEVRNVTERLVHDMWKELLDVELGQFPVMPFSEAMRRFGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVDFKVFSGPANDEKGRVAVIRVPGGASLSRKQIDEYGNFVGIYGAKGLAWMKVNDRAAG
HCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEECCCCEEEEEECCCCCC
FEGVQSPVAKFLNEEVVNAILERTQAETGDIILFGADKAGIVSEAMGALRLKLGTDLELT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHEEEECCCCEEEC
DTSAWAPLWVVDFPMFEEDGEGNLHAMHHPFTSPLGVSAEELQANPAVANSDAYDMVING
CCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEE
YEVGGGSVRIHNAEMQTAVFGILGIEAKEQQEKFGFLLEALKYGTPPHAGLAFGLDRLAM
EEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLLTDAPSLANPASLEELAIAVKLAEKKDA
HHHCCCCHHHHHCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA