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The map label for this gene is adhE [H]

Identifier: 59711525

GI number: 59711525

Start: 1012473

End: 1014974

Strand: Direct

Name: adhE [H]

Synonym: VF_0918

Alternate gene names: 59711525

Gene position: 1012473-1014974 (Clockwise)

Preceding gene: 172087679

Following gene: 59711529

Centisome position: 34.94

GC content: 42.65

Gene sequence:

>2502_bases
ATGGGTATCGTTGAAGATAAGGTTATCAAAAACCACTTCGCATCAGAGTTCATCTACAACAAATATAAAGATGAAAAAAC
ATGTGGCATTCTTGAAGAAGACGATAACCTAGGAACAATGACTATCGCAGAACCTGTAGGTATTATCTGTGGTATCGTTC
CAACAACTAACCCAACATCTACTGCAATCTTCAAATCTCTAATCTCTCTAAAAACTCGTAACGGTATCATTTTCTCGCCA
CACCCACGTGCGAAAAACTCTACTAATGATGCAGCGAAACTTGTTTTAGACGCAGCAGTTGCAGCTGGTGCTCCAAAAGA
CATCATCGGTTGGATTGATCAACCATCTGTAGAACTTTCTAATGCACTAATGAAGCACGACGGTATCGCTCTTATCCTTG
CTACTGGTGGTCCAGGCATGGTTAAAGCAGCATACTCTTCAGGTAAACCTGCAATCGGTGTTGGTGCTGGTAACGTTCCT
GTTGTTATCGATGACACAGCTGATGTTAAACGTGCAGTCGCTTCTGTTCTTATGTCTAAAACATTCGATAACGGCGTAGT
TTGTGCTTCTGAGCAAGCTGTAATCGTAATGGATGAAGTATACGACGAAGTTAAAGAGCGTTTTGCTTCTCACAAAGGTT
ACGTACTATCTAAAGCTGAAGCAGATAAAGTTCGTAAAGTTCTACTTATCGACGGTAACCTAAACGCAAACATCGTAGGT
CAACCTGCAACAGCTATCGCTGAAATGGCTGGCGTTAAAGTTCCAGCTGATACTAAGATCCTTGTTGGTGAAGGTATCGG
TGAAGTATCTTACGATGACGAATTCGCTCATGAGAAACTATCTCCAACACTAGGTATGTTCCGTGCGTCTTCTTTCGAGA
ACGCGGTAGACCAAGCTGTTAAAATGGTAGAAATTGGCGGTATCGGCCACACATCTGGTCTGTACACTAACCAAGATGTT
AACGCTGACCGTATCCGTTACTTTGGTGACAAACTAAAAACAGCACGTATTCTTGTAAACATCCCAACTACTCACGGTGG
TATCGGTGACCTTTACAACTTTAACGTTGCACCTTCTCTAACTCTTGGTTGTGGTTCATGGGGTGGTAACTCTATCTCTG
AGAACGTGGGTCCTAAGCACCTTATCAACAAGAAAACTGTAGCGAAGCGAGCTGAAAATATGTTATGGCACAAACTACCT
AAGTCTATCTACTTCCGTCGTGGCAGCCTTCCAATCGCAATGAGCGACCTAGAAGGTAAGAAACGTGCATTCCTTGTAAC
TGACCGTTTCCTATTCAACAACGGTTACGCTGATGACGTAGTTCAACTACTTAAAGCTCAAGGTATCGAAGTTCAAACAT
TCTTTGATGTAGAAGCGGATCCAACACTATCTGTTGTTGAGAAAGGCGCTGAAGCAATGAAGAGCTTCCAACCTGACGTA
ATCCTTGCTCTAGGTGGTGGTTCACCAATGGATGCTGCTAAGATTATGTGGGTAATGTACGAGCACCCAGAAACTCATTT
CGCAGAACTAGCAATGCGCTTTATGGATATCCGTAAACGTATCTACAAGTTCCCTAAAATGGGTAAAAAAGCTGAGCTTG
TATGTATCACTACAACTTCAGGTACTGGTTCAGAAGTTACTCCATTCGCTGTTGTTACAGACGACAAGACTGGTGCTAAA
TACCCACTAGCTGATTACGAAATTACGCCAAACATGGCAATCGTTGATGCTAACCTAGTAATGAACATGCCTAAGTCTCT
AACCGCATTTGGTGGTTACGATGCAGTAACTCACGCATTAGAAGCTTACGTATCTGTTCTTGCTAATGAATACTCTGACG
GTCAAGCGCTTCAAGCTCTTAAGATGCTTAAAGAATACTTACCATCAAGCTACAAAAATGGTGCGAACGACCCAATCGCT
CGTGAGAAAGTACACAATGCTGCAACTATCGCTGGTATCGCGTTTGCTAACGCATTCCTAGGTGTGTGTCACTCAATGGC
TCACAAAATCGGTGCTGAGTTCCACTTACCACACGGTTTAGCTAACGCATTACTTATCTCTAACGTTGTACGTTACAATG
CGAACGATAACCCAACTAAACAAACAGCATTCTCTCAGTACGACCGTCCACAAGCACGTCGTCGTTACGCAGAAGTTGCT
GACCACCTAGGCCTAAGCCAAGCTGGTGACCGTACTGCTCAGAAGATTGAGCGTCTACTAACTTGGTTAGAAGAGCTTAA
GAAAGATTTAGATATTCCACTATCTATCAAAGATGCAGGTGTTAACGAAGCAGACTTCATCGCTAAACTAGATGAACTAT
CTGTTGAAGCGTTCGATGACCAATGTACTGGTGCGAACCCACGTTACCCTCTAATCACAGAGCTAAAAGAAGTACTAACA
ACTTCTTACTACGGTAAACCTTTTGTTGAAGGTGAAACGTTTGAAGGCACAACAGTAATTAAAAAAACTGAAGAGCCAAA
AGCGAAAAAAGCAAAGAAATAA

Upstream 100 bases:

>100_bases
TTTTTCTCAAGAGCAAGTTGATGCAATCTTCCGTGCAGCATCTCTAGCAGCTAACCAAGCTCGTATCCCGCTAGCACAAC
AAGCGGTTGCTGAGTCTGGC

Downstream 100 bases:

>100_bases
TTGATTGCTAAACGCTGAGTTACTTTCACTAGCAATTTGAAAGTGATAAATAAGAAAGCCACCTAATATTAGGTGGCTTT
TTTGCATCTAGAGGTTTGTG

Product: fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MGIVEDKVIKNHFASEFIYNKYKDEKTCGILEEDDNLGTMTIAEPVGIICGIVPTTNPTSTAIFKSLISLKTRNGIIFSP
HPRAKNSTNDAAKLVLDAAVAAGAPKDIIGWIDQPSVELSNALMKHDGIALILATGGPGMVKAAYSSGKPAIGVGAGNVP
VVIDDTADVKRAVASVLMSKTFDNGVVCASEQAVIVMDEVYDEVKERFASHKGYVLSKAEADKVRKVLLIDGNLNANIVG
QPATAIAEMAGVKVPADTKILVGEGIGEVSYDDEFAHEKLSPTLGMFRASSFENAVDQAVKMVEIGGIGHTSGLYTNQDV
NADRIRYFGDKLKTARILVNIPTTHGGIGDLYNFNVAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLP
KSIYFRRGSLPIAMSDLEGKKRAFLVTDRFLFNNGYADDVVQLLKAQGIEVQTFFDVEADPTLSVVEKGAEAMKSFQPDV
ILALGGGSPMDAAKIMWVMYEHPETHFAELAMRFMDIRKRIYKFPKMGKKAELVCITTTSGTGSEVTPFAVVTDDKTGAK
YPLADYEITPNMAIVDANLVMNMPKSLTAFGGYDAVTHALEAYVSVLANEYSDGQALQALKMLKEYLPSSYKNGANDPIA
REKVHNAATIAGIAFANAFLGVCHSMAHKIGAEFHLPHGLANALLISNVVRYNANDNPTKQTAFSQYDRPQARRRYAEVA
DHLGLSQAGDRTAQKIERLLTWLEELKKDLDIPLSIKDAGVNEADFIAKLDELSVEAFDDQCTGANPRYPLITELKEVLT
TSYYGKPFVEGETFEGTTVIKKTEEPKAKKAKK

Sequences:

>Translated_833_residues
MGIVEDKVIKNHFASEFIYNKYKDEKTCGILEEDDNLGTMTIAEPVGIICGIVPTTNPTSTAIFKSLISLKTRNGIIFSP
HPRAKNSTNDAAKLVLDAAVAAGAPKDIIGWIDQPSVELSNALMKHDGIALILATGGPGMVKAAYSSGKPAIGVGAGNVP
VVIDDTADVKRAVASVLMSKTFDNGVVCASEQAVIVMDEVYDEVKERFASHKGYVLSKAEADKVRKVLLIDGNLNANIVG
QPATAIAEMAGVKVPADTKILVGEGIGEVSYDDEFAHEKLSPTLGMFRASSFENAVDQAVKMVEIGGIGHTSGLYTNQDV
NADRIRYFGDKLKTARILVNIPTTHGGIGDLYNFNVAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLP
KSIYFRRGSLPIAMSDLEGKKRAFLVTDRFLFNNGYADDVVQLLKAQGIEVQTFFDVEADPTLSVVEKGAEAMKSFQPDV
ILALGGGSPMDAAKIMWVMYEHPETHFAELAMRFMDIRKRIYKFPKMGKKAELVCITTTSGTGSEVTPFAVVTDDKTGAK
YPLADYEITPNMAIVDANLVMNMPKSLTAFGGYDAVTHALEAYVSVLANEYSDGQALQALKMLKEYLPSSYKNGANDPIA
REKVHNAATIAGIAFANAFLGVCHSMAHKIGAEFHLPHGLANALLISNVVRYNANDNPTKQTAFSQYDRPQARRRYAEVA
DHLGLSQAGDRTAQKIERLLTWLEELKKDLDIPLSIKDAGVNEADFIAKLDELSVEAFDDQCTGANPRYPLITELKEVLT
TSYYGKPFVEGETFEGTTVIKKTEEPKAKKAKK
>Mature_832_residues
GIVEDKVIKNHFASEFIYNKYKDEKTCGILEEDDNLGTMTIAEPVGIICGIVPTTNPTSTAIFKSLISLKTRNGIIFSPH
PRAKNSTNDAAKLVLDAAVAAGAPKDIIGWIDQPSVELSNALMKHDGIALILATGGPGMVKAAYSSGKPAIGVGAGNVPV
VIDDTADVKRAVASVLMSKTFDNGVVCASEQAVIVMDEVYDEVKERFASHKGYVLSKAEADKVRKVLLIDGNLNANIVGQ
PATAIAEMAGVKVPADTKILVGEGIGEVSYDDEFAHEKLSPTLGMFRASSFENAVDQAVKMVEIGGIGHTSGLYTNQDVN
ADRIRYFGDKLKTARILVNIPTTHGGIGDLYNFNVAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLPK
SIYFRRGSLPIAMSDLEGKKRAFLVTDRFLFNNGYADDVVQLLKAQGIEVQTFFDVEADPTLSVVEKGAEAMKSFQPDVI
LALGGGSPMDAAKIMWVMYEHPETHFAELAMRFMDIRKRIYKFPKMGKKAELVCITTTSGTGSEVTPFAVVTDDKTGAKY
PLADYEITPNMAIVDANLVMNMPKSLTAFGGYDAVTHALEAYVSVLANEYSDGQALQALKMLKEYLPSSYKNGANDPIAR
EKVHNAATIAGIAFANAFLGVCHSMAHKIGAEFHLPHGLANALLISNVVRYNANDNPTKQTAFSQYDRPQARRRYAEVAD
HLGLSQAGDRTAQKIERLLTWLEELKKDLDIPLSIKDAGVNEADFIAKLDELSVEAFDDQCTGANPRYPLITELKEVLTT
SYYGKPFVEGETFEGTTVIKKTEEPKAKKAKK

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=385, Percent_Identity=26.7532467532467, Blast_Score=90, Evalue=1e-17,
Organism=Escherichia coli, GI1787493, Length=815, Percent_Identity=77.4233128834356, Blast_Score=1325, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=371, Percent_Identity=35.3099730458221, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1789163, Length=408, Percent_Identity=30.6372549019608, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87082107, Length=371, Percent_Identity=31.5363881401617, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788797, Length=360, Percent_Identity=32.7777777777778, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1789386, Length=353, Percent_Identity=24.6458923512748, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=387, Percent_Identity=25.3229974160207, Blast_Score=114, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25143874, Length=313, Percent_Identity=23.0031948881789, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25143876, Length=313, Percent_Identity=23.0031948881789, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321181, Length=393, Percent_Identity=30.7888040712468, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24657991, Length=321, Percent_Identity=26.1682242990654, Blast_Score=80, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 90331; Mature: 90200

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIVEDKVIKNHFASEFIYNKYKDEKTCGILEEDDNLGTMTIAEPVGIICGIVPTTNPTS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECCCCCCEEECCCCCCCHH
TAIFKSLISLKTRNGIIFSPHPRAKNSTNDAAKLVLDAAVAAGAPKDIIGWIDQPSVELS
HHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHH
NALMKHDGIALILATGGPGMVKAAYSSGKPAIGVGAGNVPVVIDDTADVKRAVASVLMSK
HHHHHCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHH
TFDNGVVCASEQAVIVMDEVYDEVKERFASHKGYVLSKAEADKVRKVLLIDGNLNANIVG
CCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEEC
QPATAIAEMAGVKVPADTKILVGEGIGEVSYDDEFAHEKLSPTLGMFRASSFENAVDQAV
CCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMVEIGGIGHTSGLYTNQDVNADRIRYFGDKLKTARILVNIPTTHGGIGDLYNFNVAPSL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCE
TLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLPKSIYFRRGSLPIAMSDLEGK
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCCCC
KRAFLVTDRFLFNNGYADDVVQLLKAQGIEVQTFFDVEADPTLSVVEKGAEAMKSFQPDV
CEEEEEEEHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCE
ILALGGGSPMDAAKIMWVMYEHPETHFAELAMRFMDIRKRIYKFPKMGKKAELVCITTTS
EEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
GTGSEVTPFAVVTDDKTGAKYPLADYEITPNMAIVDANLVMNMPKSLTAFGGYDAVTHAL
CCCCCCCCEEEEECCCCCCCCCCCCEEECCCEEEEECHHHHCCCCHHHHCCCHHHHHHHH
EAYVSVLANEYSDGQALQALKMLKEYLPSSYKNGANDPIAREKVHNAATIAGIAFANAFL
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVCHSMAHKIGAEFHLPHGLANALLISNVVRYNANDNPTKQTAFSQYDRPQARRRYAEVA
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHH
DHLGLSQAGDRTAQKIERLLTWLEELKKDLDIPLSIKDAGVNEADFIAKLDELSVEAFDD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLITELKEVLTTSYYGKPFVEGETFEGTTVIKKTEEPKAKKAKK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHCCCC
>Mature Secondary Structure 
GIVEDKVIKNHFASEFIYNKYKDEKTCGILEEDDNLGTMTIAEPVGIICGIVPTTNPTS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECCCCCCEEECCCCCCCHH
TAIFKSLISLKTRNGIIFSPHPRAKNSTNDAAKLVLDAAVAAGAPKDIIGWIDQPSVELS
HHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHH
NALMKHDGIALILATGGPGMVKAAYSSGKPAIGVGAGNVPVVIDDTADVKRAVASVLMSK
HHHHHCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHH
TFDNGVVCASEQAVIVMDEVYDEVKERFASHKGYVLSKAEADKVRKVLLIDGNLNANIVG
CCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEEC
QPATAIAEMAGVKVPADTKILVGEGIGEVSYDDEFAHEKLSPTLGMFRASSFENAVDQAV
CCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMVEIGGIGHTSGLYTNQDVNADRIRYFGDKLKTARILVNIPTTHGGIGDLYNFNVAPSL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCE
TLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLPKSIYFRRGSLPIAMSDLEGK
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCCCC
KRAFLVTDRFLFNNGYADDVVQLLKAQGIEVQTFFDVEADPTLSVVEKGAEAMKSFQPDV
CEEEEEEEHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCE
ILALGGGSPMDAAKIMWVMYEHPETHFAELAMRFMDIRKRIYKFPKMGKKAELVCITTTS
EEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
GTGSEVTPFAVVTDDKTGAKYPLADYEITPNMAIVDANLVMNMPKSLTAFGGYDAVTHAL
CCCCCCCCEEEEECCCCCCCCCCCCEEECCCEEEEECHHHHCCCCHHHHCCCHHHHHHHH
EAYVSVLANEYSDGQALQALKMLKEYLPSSYKNGANDPIAREKVHNAATIAGIAFANAFL
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVCHSMAHKIGAEFHLPHGLANALLISNVVRYNANDNPTKQTAFSQYDRPQARRRYAEVA
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHH
DHLGLSQAGDRTAQKIERLLTWLEELKKDLDIPLSIKDAGVNEADFIAKLDELSVEAFDD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLITELKEVLTTSYYGKPFVEGETFEGTTVIKKTEEPKAKKAKK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]