Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 59711431

GI number: 59711431

Start: 904854

End: 906065

Strand: Direct

Name: sucB [H]

Synonym: VF_0824

Alternate gene names: 59711431

Gene position: 904854-906065 (Clockwise)

Preceding gene: 59711430

Following gene: 59711432

Centisome position: 31.23

GC content: 41.34

Gene sequence:

>1212_bases
ATGACAATCGAAATTCTGGTTCCAGATTTACCTGAATCTGTAGCAGACGCTACTGTAGCAACATGGCATAAACAACCTGG
TGATGCAGTTGAGCGTGATGAAATACTTGTAGACATTGAAACGGACAAAGTGGTTCTTGAAGTACCAGCGCCAGAAGCGG
GAGTACTAGAAACCATTTTAGAAGATGAAGGTGCAACGGTTCTTTCTAAACAACTTCTTGCGCGTATTAAGCCAGGTGCT
GTTGTTGGTGAGCCAACGACTGATGTAACAACGGCAACTGAATCATCTCCTGATAAACGTCATACAGCGTCTTTATCAGA
AGAAAGCAATGATGCATTAAGTCCTGCGGTTCGTCGCCTATTAGGTGAGCATGATATTGCTCCATCTGATGTGAAAGGCA
CAGGTGTTGGTGGTCGCATTACACGTGAAGATGTTGATGCGCATATTGCAGCATTGAAAGCCGCACCAAAAGCAGAAAAA
GCAGCAGATAAGCCAGCAGAAGCATTAGCTCATCGTAGCGAAAAACGTGTACCGATGACTCGCCTTCGTAAAACAGTAGC
CAACCGTCTACTTGAAGCGAAGAACAGCACAGCGATGTTAACGACGTTTAATGAAGTAAACATGAAACCTATCATGGATC
TTCGTAAGCAATATAAAGATCAATTTGAAGAGCGTCATGGCACACGTTTAGGCTTTATGTCTTTCTATGTGAAAGCGGTA
ACAGAAGCATTGAAGCGTTACCCAGAAGTGAATGCGTCAATTGATGGTGATGATATCGTTTATCATAACTACTTTGATAT
TAGTATGGCAGTTTCAACTCCTCGTGGGTTAGTTACTCCTGTGCTGAAAGATTGCGATACGCTTGGATTTGCTGATATTG
AAAAAGGCATTAAAGAGCTAGCAATTAAAGGTCGTGACGGTAAATTAGCAGTAGAAGATTTAATGGGCGGAAACTTTACC
ATTACTAATGGTGGCGTGTTTGGTTCATTAATGTCGACGCCAATTATCAATCCACCTCAAGCTGCAATTTTAGGTATGCA
TAAAATCCAAGATCGTCCTATGGCGGTTGATGGTAAGGTAGAAATTTTACCAATGATGTATCTAGCATTGTCTTACGATC
ACCGTTTAATTGATGGTCGTGAATCAGTAGGTTTCTTAGTAACCATTAAAGAGTTACTTGAAGATCCTGCTCGTTTATTA
CTAGATGTTTAA

Upstream 100 bases:

>100_bases
CCAGCAGTAGGTTATATGTCGGTGCACGTGAAACAACAAAAAGCGTTAGTAGAAGACGCTCTGACCCTAGATAAGAACTA
GAAAGTATAGGAAAACGGAT

Downstream 100 bases:

>100_bases
ATATAACGTTTAGTGACAAACAAAGGCTGCCATGCTCACGGCAGCCTCTTATTCAACTTCATTATTGGATAAAAAATAGA
AAGAGACCTTAGAGCCTCTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTIEILVPDLPESVADATVATWHKQPGDAVERDEILVDIETDKVVLEVPAPEAGVLETILEDEGATVLSKQLLARIKPGA
VVGEPTTDVTTATESSPDKRHTASLSEESNDALSPAVRRLLGEHDIAPSDVKGTGVGGRITREDVDAHIAALKAAPKAEK
AADKPAEALAHRSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEERHGTRLGFMSFYVKAV
TEALKRYPEVNASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLAVEDLMGGNFT
ITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLL
LDV

Sequences:

>Translated_403_residues
MTIEILVPDLPESVADATVATWHKQPGDAVERDEILVDIETDKVVLEVPAPEAGVLETILEDEGATVLSKQLLARIKPGA
VVGEPTTDVTTATESSPDKRHTASLSEESNDALSPAVRRLLGEHDIAPSDVKGTGVGGRITREDVDAHIAALKAAPKAEK
AADKPAEALAHRSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEERHGTRLGFMSFYVKAV
TEALKRYPEVNASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLAVEDLMGGNFT
ITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLL
LDV
>Mature_402_residues
TIEILVPDLPESVADATVATWHKQPGDAVERDEILVDIETDKVVLEVPAPEAGVLETILEDEGATVLSKQLLARIKPGAV
VGEPTTDVTTATESSPDKRHTASLSEESNDALSPAVRRLLGEHDIAPSDVKGTGVGGRITREDVDAHIAALKAAPKAEKA
ADKPAEALAHRSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEERHGTRLGFMSFYVKAVT
EALKRYPEVNASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLAVEDLMGGNFTI
TNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLL
DV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=252, Percent_Identity=56.7460317460317, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=28.604118993135, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=28.1995661605206, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=28.1995661605206, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=28.0092592592593, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=76.6749379652606, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1786305, Length=409, Percent_Identity=31.2958435207824, Blast_Score=161, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=43.3583959899749, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=30.8924485125858, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=25.5369928400955, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=31.875, Blast_Score=140, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=423, Percent_Identity=42.080378250591, Blast_Score=316, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=27.3730684326711, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=56.2248995983936, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=27.6255707762557, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=31.8777292576419, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43923; Mature: 43792

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKQPGDAVERDEILVDIETDKVVLEVPAPEAGVLETIL
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EDEGATVLSKQLLARIKPGAVVGEPTTDVTTATESSPDKRHTASLSEESNDALSPAVRRL
HCCCCHHHHHHHHHHCCCCCEECCCCCCCEECCCCCCCHHHHHCCCCCCCCHHHHHHHHH
LGEHDIAPSDVKGTGVGGRITREDVDAHIAALKAAPKAEKAADKPAEALAHRSEKRVPMT
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCHH
RLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEERHGTRLGFMSFYVKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEALKRYPEVNASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKEL
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHH
AIKGRDGKLAVEDLMGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKV
EECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCC
EILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
HHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKQPGDAVERDEILVDIETDKVVLEVPAPEAGVLETIL
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EDEGATVLSKQLLARIKPGAVVGEPTTDVTTATESSPDKRHTASLSEESNDALSPAVRRL
HCCCCHHHHHHHHHHCCCCCEECCCCCCCEECCCCCCCHHHHHCCCCCCCCHHHHHHHHH
LGEHDIAPSDVKGTGVGGRITREDVDAHIAALKAAPKAEKAADKPAEALAHRSEKRVPMT
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCHH
RLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEERHGTRLGFMSFYVKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEALKRYPEVNASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKEL
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHH
AIKGRDGKLAVEDLMGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKV
EECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCC
EILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
HHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]