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The map label for this gene is thiI

Identifier: 59711323

GI number: 59711323

Start: 783047

End: 784495

Strand: Direct

Name: thiI

Synonym: VF_0716

Alternate gene names: 59711323

Gene position: 783047-784495 (Clockwise)

Preceding gene: 59711322

Following gene: 59711331

Centisome position: 27.02

GC content: 38.37

Gene sequence:

>1449_bases
ATGAAGTTTATCGTTAAGCCACACCCAGAAGTTTTTGTTAAAAGTGATTCAGTACGTAAGCGCTTTATTCGTATTCTTGA
ATCTAACCTACGCAGTATTATTCAGCGTGAAACCAAAGGTGTTGAAGTCATCAACCGTCGTGATCATATCGAAGTGACAG
GACTGAGTGATGAATACCGTGATGTAACATTGGCGGTACTAACGCAAACTCCAGGTATTCATCATGTACTTGAAGTTAAG
CAGTCAGGCTTTAAAGACATGCACGATATTTTTGAGCAATGTCTTGAAATGAACCGTGAAGTGATTGAAGGTAAAACGTT
CTGTGTACGTGTTAAGCGTCGTGGTAATCATGACTTTACGTCTATTGAGCTTGAGCGCTACGTTGGTGGCGGTTTAAATC
AATCAGTAGAAAGTGCAAGTGTTCGTCTAAAGAACCCTGAAGTGACGGTTAAATTTGAAGTAGCAAATGATAAATTAAAC
TTGATCATTGCTCGTCATAAAGGCTTGGGTGGTTTTCCTCTTGGAACTCAAGAAGATGTATTAAGCTTGATTTCTGGTGG
TTTTGATTCTGGTGTATCAAGCTACTTACACATTAAACGTGGTTCAAAAGTACACTACTTATTCTTTAATTTAGGTGGTC
CAGCTCACGAGATTGGCGTGAAACAAGTGTCGCACTTCTTGTGGAAAAAATACGGTTCATCAGCAAAAGTTAAATTTATC
TCTGTTGATTTTGATCCAGTTGTTGCTGAGATCTTAGAGAAAGTCGATGATGGTCAAATGGGTGTTATCTTAAAACGTAT
GTTTATGCGTGCAGGTGGCATGGTTGCTGAGAAATTTGGCATTGAAGGATTAGTTACTGGTGAAGCATTAGGTCAAGTAT
CTAGTCAAACACTAACTAACTTACGCCATATTGATAATGTAACTGATTCACTGATTCTACGTCCTCTAATTAACTGGGAC
AAAGAAGATATTATTGATTTAGCTCGTGAGATTGGCACTGAAGATTTCGCTAAAACAATGCCTGAATACTGTGGTGTAAT
TTCTAAGAAACCAACAGTAAAAGCAGTAAAAGAAAAACTAGAAAAAGAAGAAGCGAAATTCGATTTCTCTGTTCTTGAGC
AAGCGGTTTATAACGCGCGAGTTATGGACATTCGTGATATTGAAAAAGAAAGCCAAGAGCAAGCTCCAGAAGTTGAAATG
GTATCTGAGCTAGGTTCTGATGTTGTGGTTCTTGATATCCGTAGTGCTGAAGAAGAAGATGAGAAGCCATTAGAGCTTGA
TGGTGTTGAGGTGACTCACATTCCTTTCTTCAAATTAGCAACTAAGTTTGGTGATCTAGACCAATCTAAAGAGTACTTAT
TATACTGTGAGCGTGGTGTGATGAGCCGTCTTCAAGCATTATTATTAATTGAGAATGGCTATAAGAACGTAAAAGTTTAC
CGTCCATAG

Upstream 100 bases:

>100_bases
ACTCGCCTTTTTTTTCGGTATAATCTTGCGCCTTAGATTAGGTCCTGAACCAACCTTATCTATCCTTTTGATTGTTTTCC
TACATCAGTGAAGTCTATAT

Downstream 100 bases:

>100_bases
TTCGTAAATATGTAATATTTAAAAAGGGCGTTGATTATTCAACGCCCTTTTTTTGTCTATCACCGATTGGAGTTAGACTT
GATGATAATTAAGATTAGGA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYRDVTLAVLTQTPGIHHVLEVK
QSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFTSIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLN
LIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTNLRHIDNVTDSLILRPLINWD
KEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEM
VSELGSDVVVLDIRSAEEEDEKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
RP

Sequences:

>Translated_482_residues
MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYRDVTLAVLTQTPGIHHVLEVK
QSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFTSIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLN
LIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTNLRHIDNVTDSLILRPLINWD
KEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEM
VSELGSDVVVLDIRSAEEEDEKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
RP
>Mature_482_residues
MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYRDVTLAVLTQTPGIHHVLEVK
QSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFTSIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLN
LIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTNLRHIDNVTDSLILRPLINWD
KEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEM
VSELGSDVVVLDIRSAEEEDEKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=63.0705394190871, Blast_Score=634, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_VIBF1 (Q5E6Y5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204099.1
- ProteinModelPortal:   Q5E6Y5
- STRING:   Q5E6Y5
- GeneID:   3278292
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0716
- NMPDR:   fig|312309.3.peg.1217
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   VFIS312309:VF0716-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54475; Mature: 54475

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 456-456 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYR
CCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCEEEECCCCCHH
DVTLAVLTQTPGIHHVLEVKQSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFT
CEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCEEEEEEEECCCCCCE
SIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLNLIIARHKGLGGFPLGTQEDV
EEEHHHHHCCCCCCCHHHCEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTN
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCEEEEHHHHHHHHHHHHHH
LRHIDNVTDSLILRPLINWDKEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
EKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEMVSELGSDVVVLDIRSAEEED
HHHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCC
EKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
CCCCEECCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYR
CCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCEEEECCCCCHH
DVTLAVLTQTPGIHHVLEVKQSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFT
CEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCEEEEEEEECCCCCCE
SIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLNLIIARHKGLGGFPLGTQEDV
EEEHHHHHCCCCCCCHHHCEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTN
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCEEEEHHHHHHHHHHHHHH
LRHIDNVTDSLILRPLINWDKEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
EKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEMVSELGSDVVVLDIRSAEEED
HHHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCC
EKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
CCCCEECCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA