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The map label for this gene is nanK [H]

Identifier: 59711274

GI number: 59711274

Start: 730821

End: 731696

Strand: Direct

Name: nanK [H]

Synonym: VF_0667

Alternate gene names: 59711274

Gene position: 730821-731696 (Clockwise)

Preceding gene: 59711273

Following gene: 59711275

Centisome position: 25.22

GC content: 42.81

Gene sequence:

>876_bases
ATGAAGCCTTGCTTAACGGTTGATATTGGTGGAACTAAAATTGCAGCGGCAATCATTGAATCTGGAAAAGTGCTACGCCG
TCAGCAAATTGCGACACCATCGTCTAGCCAACCAGAAGAGATGGATAAAGCGTTAGAGGAACTGTTAACGCCATTTTTAG
ATGATATTTCAACGGTTGCTGTAGCATCAACTGGGATCATTGATGATGGAGTATTAACTGCTTTAAATCCGTTAAATCTG
GGTGGATTAAATAACTATCCACTAAGAGCTGTGATTGAAAAAATCACGAAAAAGCCAACAACAGTCATTAATGATGCGCA
AGCTGCCGCATGGGCTGAGTACCAAACACTTGAGTTAAATATGGTTAACATGGCTTTTATTACCGTATCTACTGGCGTTG
GTGCTGGCGTTGTTATTAATGATGATTTGCTTATCGGTGCGAATGGTATTGCAGGTCACGCAGGACATACGCTTGCGGAT
CCTAATGGACCAATATGTGGTTGTGGTCGTCGAGGCTGTGTTGAGAGTATTGCTTCAGGTACAGCGATAGGCCAAGCCGG
AAAAACCTATTTTGGCGATAACTGCACAGGGGAAATGGTTTTTAAACATTTTTCGCAAAATGACCCTAATGCCACAGATA
TCGTAAACGGTTCAGCTAAGGCGATAGCCAACTTAATTGCCGATTTAAAAATGGTATTAGATATTGAATTGGTCGCTTTA
GGAGGCAGTGTTGGTCTTGCTCCTCATTATTTAGAACTCGTACAGCATTACCTAGCACAACAACCTTCACCATACCAAAC
CAAGGTTCAGCATGCTCGTTGCGGCGCTGATGCAGGATTGATTGGTGTCGCTCATTGGGCTAGCAAAATACGTTAA

Upstream 100 bases:

>100_bases
GCGTCACTGTTGGTTCAGCGATTACACGTATTGAGCATATTTGTGGTTGGTTTAAATCTGAAGTTGAACTAGGTAAGAAA
AACATGGTTAAGGACATCGC

Downstream 100 bases:

>100_bases
AAAAGAACTTCTAAGAAATAGTAATTTAACCCTACAAAAATTATACAAGGTATATTCCGATGGAAGCACAATCATTTGGT
ACATTAAATTACATTGCTCT

Product: N-acetylmannosamine kinase

Products: NA

Alternate protein names: ManNAc kinase; N-acetyl-D-mannosamine kinase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKPCLTVDIGGTKIAAAIIESGKVLRRQQIATPSSSQPEEMDKALEELLTPFLDDISTVAVASTGIIDDGVLTALNPLNL
GGLNNYPLRAVIEKITKKPTTVINDAQAAAWAEYQTLELNMVNMAFITVSTGVGAGVVINDDLLIGANGIAGHAGHTLAD
PNGPICGCGRRGCVESIASGTAIGQAGKTYFGDNCTGEMVFKHFSQNDPNATDIVNGSAKAIANLIADLKMVLDIELVAL
GGSVGLAPHYLELVQHYLAQQPSPYQTKVQHARCGADAGLIGVAHWASKIR

Sequences:

>Translated_291_residues
MKPCLTVDIGGTKIAAAIIESGKVLRRQQIATPSSSQPEEMDKALEELLTPFLDDISTVAVASTGIIDDGVLTALNPLNL
GGLNNYPLRAVIEKITKKPTTVINDAQAAAWAEYQTLELNMVNMAFITVSTGVGAGVVINDDLLIGANGIAGHAGHTLAD
PNGPICGCGRRGCVESIASGTAIGQAGKTYFGDNCTGEMVFKHFSQNDPNATDIVNGSAKAIANLIADLKMVLDIELVAL
GGSVGLAPHYLELVQHYLAQQPSPYQTKVQHARCGADAGLIGVAHWASKIR
>Mature_291_residues
MKPCLTVDIGGTKIAAAIIESGKVLRRQQIATPSSSQPEEMDKALEELLTPFLDDISTVAVASTGIIDDGVLTALNPLNL
GGLNNYPLRAVIEKITKKPTTVINDAQAAAWAEYQTLELNMVNMAFITVSTGVGAGVVINDDLLIGANGIAGHAGHTLAD
PNGPICGCGRRGCVESIASGTAIGQAGKTYFGDNCTGEMVFKHFSQNDPNATDIVNGSAKAIANLIADLKMVLDIELVAL
GGSVGLAPHYLELVQHYLAQQPSPYQTKVQHARCGADAGLIGVAHWASKIR

Specific function: Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NanK subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082230, Length=266, Percent_Identity=50.7518796992481, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1787363, Length=297, Percent_Identity=25.9259259259259, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081733, Length=298, Percent_Identity=25.1677852348993, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082127, Length=164, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786891, Length=243, Percent_Identity=24.2798353909465, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.60 [H]

Molecular weight: Translated: 30397; Mature: 30397

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPCLTVDIGGTKIAAAIIESGKVLRRQQIATPSSSQPEEMDKALEELLTPFLDDISTVA
CCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VASTGIIDDGVLTALNPLNLGGLNNYPLRAVIEKITKKPTTVINDAQAAAWAEYQTLELN
HHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHEEEEEE
MVNMAFITVSTGVGAGVVINDDLLIGANGIAGHAGHTLADPNGPICGCGRRGCVESIASG
EEEEEEEEEECCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCC
TAIGQAGKTYFGDNCTGEMVFKHFSQNDPNATDIVNGSAKAIANLIADLKMVLDIELVAL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEEEEEE
GGSVGLAPHYLELVQHYLAQQPSPYQTKVQHARCGADAGLIGVAHWASKIR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKPCLTVDIGGTKIAAAIIESGKVLRRQQIATPSSSQPEEMDKALEELLTPFLDDISTVA
CCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VASTGIIDDGVLTALNPLNLGGLNNYPLRAVIEKITKKPTTVINDAQAAAWAEYQTLELN
HHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHEEEEEE
MVNMAFITVSTGVGAGVVINDDLLIGANGIAGHAGHTLADPNGPICGCGRRGCVESIASG
EEEEEEEEEECCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCC
TAIGQAGKTYFGDNCTGEMVFKHFSQNDPNATDIVNGSAKAIANLIADLKMVLDIELVAL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEEEEEE
GGSVGLAPHYLELVQHYLAQQPSPYQTKVQHARCGADAGLIGVAHWASKIR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA