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The map label for this gene is ascG [H]

Identifier: 59711215

GI number: 59711215

Start: 666974

End: 667978

Strand: Direct

Name: ascG [H]

Synonym: VF_0608

Alternate gene names: 59711215

Gene position: 666974-667978 (Clockwise)

Preceding gene: 59711214

Following gene: 59711216

Centisome position: 23.02

GC content: 34.83

Gene sequence:

>1005_bases
ATGGCAACAATCAATGACGTATGTAAGCTTGCAGGTGTTTCTAAAGCGACCGTTTCTCGAGTAATGAATGGAAAAGGTCC
AGTAAAAGAAGAAACAAAAAAACTGATTTTTGATGCAATGGAAACATTAGACTATAAACCGAGCCTTGTCGCTCAAGCAT
TGGCAACAAATAGTTCTAATAGTATTGGCCTTATTGTGTCGTTCTTTGATGGTTATTATTTTGGTATTTTACTCAAAGAA
GCGACAAGAATTGCAGAAGAATCAGGAAAGCAATTAATCGTCACTGACGGTCATAATCAAGTTGAAACTGAGATCGAAGC
CATTAATTCACTAGTGGCAAGAAAGTGTGATGCTATTTTAATTTATAGTCGCACGATGACGGCTGATGATTATTGCGAAA
TTCAGAAAAAACTGCCGATCCCACTTGTTGTTATTAATCGAACCTTACCTGATGGCTTAGGGCACGCAGTTGCTTTTGAC
CAATATAATGCAAGTCAAATGGCTATTGAGCATCTTGTTGGATTAGGGCATAAAAACATTGCGATTATCACCCTTAATAT
GACATCAAGTACTGGTCAAATTCGCTTGAAAGGAGCGAAAGAAACGCTTGAAAAACATGGTATTGCTTATGATGATAACT
TAACCATAATAAAGAATGGTAAGTTAGAAGATGGGTATCAAGCATGTAAAGAGTTGCTGAAGCGTTCACAAGATATTTCT
GCTATTTTTTGTTTTAATGATCATTTAGCACTCGGTGCAATTAAAGCCTTAAAAGAGATGAATATTCGTGTTCCTGAAGA
TATTTCCATCGTTGGGATAGATAATGATGAGATTTCTGCATTTTTATCTCCAGCGTTAACAACGATTAATATTCCTATTC
AAAAAGTAACTCAGTTGGCAATGGATAAAGCATTGAGATTGATTTCTGATAAAGAAGTAAAAGCAACAACTGAAATATTA
AAAGGTAATATAATTGTTCGTGAATCAACAAAGAATATTAATTAA

Upstream 100 bases:

>100_bases
TGAGATTCATCGCGCGTTGCAGAATAAATAATAAAGTGTATTTAGCGTAATAACTTAGCTATTATTACGCTAATTTCATC
TACATATTTTTGAGGGCTCA

Downstream 100 bases:

>100_bases
ACTATTTTATTTTTAATTAAGCATCGATTAATTGGTGCTTTTTTATTTTTATTGGATAGATACCTCTTAAAAAGCTAAAA
ATAAAATATATATAAATATA

Product: LacI family DNA-binding transcriptional repressor

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLDYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKE
ATRIAEESGKQLIVTDGHNQVETEIEAINSLVARKCDAILIYSRTMTADDYCEIQKKLPIPLVVINRTLPDGLGHAVAFD
QYNASQMAIEHLVGLGHKNIAIITLNMTSSTGQIRLKGAKETLEKHGIAYDDNLTIIKNGKLEDGYQACKELLKRSQDIS
AIFCFNDHLALGAIKALKEMNIRVPEDISIVGIDNDEISAFLSPALTTINIPIQKVTQLAMDKALRLISDKEVKATTEIL
KGNIIVRESTKNIN

Sequences:

>Translated_334_residues
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLDYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKE
ATRIAEESGKQLIVTDGHNQVETEIEAINSLVARKCDAILIYSRTMTADDYCEIQKKLPIPLVVINRTLPDGLGHAVAFD
QYNASQMAIEHLVGLGHKNIAIITLNMTSSTGQIRLKGAKETLEKHGIAYDDNLTIIKNGKLEDGYQACKELLKRSQDIS
AIFCFNDHLALGAIKALKEMNIRVPEDISIVGIDNDEISAFLSPALTTINIPIQKVTQLAMDKALRLISDKEVKATTEIL
KGNIIVRESTKNIN
>Mature_333_residues
ATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLDYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKEA
TRIAEESGKQLIVTDGHNQVETEIEAINSLVARKCDAILIYSRTMTADDYCEIQKKLPIPLVVINRTLPDGLGHAVAFDQ
YNASQMAIEHLVGLGHKNIAIITLNMTSSTGQIRLKGAKETLEKHGIAYDDNLTIIKNGKLEDGYQACKELLKRSQDISA
IFCFNDHLALGAIKALKEMNIRVPEDISIVGIDNDEISAFLSPALTTINIPIQKVTQLAMDKALRLISDKEVKATTEILK
GNIIVRESTKNIN

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=38.1381381381381, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789202, Length=329, Percent_Identity=33.1306990881459, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=32.3529411764706, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787948, Length=321, Percent_Identity=29.9065420560748, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=27.6190476190476, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=28.9156626506024, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=29.9684542586751, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=28.1437125748503, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=26.1904761904762, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=23.1511254019293, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=24.6987951807229, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=290, Percent_Identity=24.1379310344828, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=27.3927392739274, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=22.6837060702875, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36565; Mature: 36434

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLDYKPSLVAQALATNSSN
CCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
SIGLIVSFFDGYYFGILLKEATRIAEESGKQLIVTDGHNQVETEIEAINSLVARKCDAIL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
IYSRTMTADDYCEIQKKLPIPLVVINRTLPDGLGHAVAFDQYNASQMAIEHLVGLGHKNI
EEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCE
AIITLNMTSSTGQIRLKGAKETLEKHGIAYDDNLTIIKNGKLEDGYQACKELLKRSQDIS
EEEEEEECCCCCEEEECCHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHHCCCCCE
AIFCFNDHLALGAIKALKEMNIRVPEDISIVGIDNDEISAFLSPALTTINIPIQKVTQLA
EEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCHHHHHHHH
MDKALRLISDKEVKATTEILKGNIIVRESTKNIN
HHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure 
ATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLDYKPSLVAQALATNSSN
CCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
SIGLIVSFFDGYYFGILLKEATRIAEESGKQLIVTDGHNQVETEIEAINSLVARKCDAIL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
IYSRTMTADDYCEIQKKLPIPLVVINRTLPDGLGHAVAFDQYNASQMAIEHLVGLGHKNI
EEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCE
AIITLNMTSSTGQIRLKGAKETLEKHGIAYDDNLTIIKNGKLEDGYQACKELLKRSQDIS
EEEEEEECCCCCEEEECCHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHHCCCCCE
AIFCFNDHLALGAIKALKEMNIRVPEDISIVGIDNDEISAFLSPALTTINIPIQKVTQLA
EEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCHHHHHHHH
MDKALRLISDKEVKATTEILKGNIIVRESTKNIN
HHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]