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The map label for this gene is clpB [H]

Identifier: 59711173

GI number: 59711173

Start: 614013

End: 616598

Strand: Direct

Name: clpB [H]

Synonym: VF_0566

Alternate gene names: 59711173

Gene position: 614013-616598 (Clockwise)

Preceding gene: 59711172

Following gene: 59711179

Centisome position: 21.19

GC content: 37.97

Gene sequence:

>2586_bases
ATGCGTTTAGATCGATTCACTAGTAAATTTCAAATGGCCATCTCTGATGCTCAATCATTAGCATTAGGTCAAGATCATCA
ATATATCGAACCAACCCACTTAATGGTTGCACTGTTAAATCAGGATGGAAGTACGATAAGGCCTTTACTTACTCTTCTAA
ATGTAGACGTAACACAATTACGTTCAAAACTAACTGAAATATTAGATAAGACACCAAAGGTAACGGGTATCGGTGGTGAT
GTTCAACTTTCATCTAATATGGGTGTTATTTTTAATCTTTGCGATAAAGTCGCTCAGAAACGCAAAGACGCTTATATCTC
TTCTGAAATATTCATGCTTGCTGCAATCGAAGATAAAGGGCCTTTGGGTAATTTACTTCGAGAATTAGGATTAACTGAAC
CTAAAATATCTAAGTCAATCGATGAAATCCGTGGTGGCGAAAAAGTAAATGATCAAAATGCTGAAGAAAAGCGTCAAGCA
TTAGAAAAGTTTACTGTTGATTTAACTGAGCGAGCAGAGCAAGGAAAATTAGATCCTGTAATTGGTCGTGATGATGAAAT
ACGAAGAACAATTCAGGTATTGCAACGTCGAACTAAAAATAATCCAGTTATCATAGGTCAACCTGGTGTCGGCAAGACAG
CTATTGTTGAAGGGTTGGCGCAACGTATTATAAATGGTGAAGTACCTGAAGGTTTAAAAAATAAACGAGTACTTTCTTTA
GATATTGGTGCATTGGTCGCTGGTGCTAAATTCCGAGGTGAATTTGAAGAGCGCTTAAAAGCGGTTTTAAATGAGCTAGC
GAAAGAAGAAGGTAGTGTCATCCTCTTTATTGATGAAGTACATACAATGGTAGGTGCTGGTAAAGGTGAAGGCTCTATGG
ATGCTGGCAATATGCTTAAACCTGCACTCGCACGTGGTGAACTACATTGTGTCGGAGCAACAACTCTTGATGAATATCGT
CAGTATATTGAAAAAGATCCAGCATTAGAGCGTCGTTTTCAAAAAGTACTTGTTGATGAACCAACAGTTGAAGATACCGT
TGCAATTTTACGTGGTTTAAAAGAACGCTATGAAATTCATCATCATGTTGAAATTACCGATCCTGCGATCGTAGCTGCAG
CAAGCTTGTCACATCGTTATATATCGGATCGTCAATTACCGGATAAAGCGATAGATTTAATTGACGAAGCAGCATCAAGC
ATTCGTATGGAAATTGATTCAAAACCAGAATCATTAGATAAGCTTGATCGTAAGATAATTCAATTAAAGATAGAACAGCA
AGCATTAGTAAAAGAAAGCGATGATGCGAGCTTAAAGCGTCTTGACTCTTTAAATCTTGAGTTAATGCAAAAAGAACGTG
AATACGCAGAGCTTGAAGAAGTTTGGAAAGCTGAAAAAGCGGCTTTATCTGGCACTCAGCATATAAAAACAGAATTAGAA
ACCGCTCGAAGTAATATGGAAATTGCACGTCGTGCAGGTGATCTAAATAGAATGTCAGAGCTGCAATATGGACGTATCCC
TGAATTAGAGAAACAACTGGACCTTGCAGCTCAAGCTGAAATGCAAGAAATGAGCTTATTAAAAAATAAAGTGACGGATG
CTGAGATAGCGGAAGTGCTTTCACGTCAGACGGGCATTCCTGTAAATAAAATGCTTGAAGGTGAAAGAGATAAGCTATTA
AAGATGGAAGAGGTATTACATCACCGAGTAATAGGCCAGGCTGAAGCTGTCGAAGCTGTCTCAAATGCAATTCGTCGTAG
TCGTGCCGGTCTATCAGATCCAAATAGACCAATTGGTTCATTCTTGTTTTTAGGCCCAACAGGGGTTGGTAAAACGGAAT
TATGTAAATCACTCGCTAACTTTATGTTTGATAGTGAAGATGCGATGGTACGTATCGACATGTCTGAGTTTATGGAGAAA
CATTCCGTTGCTCGTTTAGTAGGTGCACCTCCTGGCTATGTTGGTTATGAAGAGGGTGGATACTTAACAGAAGCAGTAAG
ACGCAAACCTTATTCAGTTTTATTATTAGATGAAGTAGAGAAAGCACATCCAGATGTATTTAATATTCTACTTCAAGTTC
TTGATGATGGGCGCTTAACTGATGGTCAGGGAAGAACGGTTGATTTTAAAAATACCGTTATCATTATGACATCGAACTTA
GGTTCAGAAAAAATTCAGCAACATTTTGGTGAGTTGAATTATGCTGGCATAAAAGAAGTCGTAATGGATGTTGTGAGTCA
ACATTTTAGACCAGAGTTTTTAAACCGTGTTGATGAAACCGTAGTATTCCATCCATTGGCACAAGAACACATTAAGAATA
TCGCTTCTATTCAATTACAACGCTTAGAAAAACGTTTGAATGAAAAAGATTACCAATTGCAAGTAACAGACGAAGCGTTA
AACTTAATTGCTGAGGCAGGTTTTGATCCTGTTTATGGAGCAAGACCATTAAAACGAGCAATTCAGACATATATAGAGAA
CCCACTTGCTCAGGATATATTGAGTGGAAAGCTAAGGGTTGGAGAAGTAATTAAGTTGAAAGTTAAAAATGAACAGCTAA
TTGCGACTCAAAATGACGACTTTTGA

Upstream 100 bases:

>100_bases
ATTTCTACCCCTTTATCCTTTCCTTAAGTATTGAACTTTCTGACCTTAGCCCCCATTTAGAAACTATATAGTTACTAGCT
TTCTTCTTTTGGAGGGTGTT

Downstream 100 bases:

>100_bases
TGTAAAAATAAACGCTTTGAATTAAAAGTAAGCAAACAAACTCATTTTTCATATTTTTCTAGAAAAAGAGTTGCATAGAT
TCAAAGACTCTCTATAATGC

Product: protein disaggregation chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MRLDRFTSKFQMAISDAQSLALGQDHQYIEPTHLMVALLNQDGSTIRPLLTLLNVDVTQLRSKLTEILDKTPKVTGIGGD
VQLSSNMGVIFNLCDKVAQKRKDAYISSEIFMLAAIEDKGPLGNLLRELGLTEPKISKSIDEIRGGEKVNDQNAEEKRQA
LEKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DIGALVAGAKFRGEFEERLKAVLNELAKEEGSVILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTVAILRGLKERYEIHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLDRKIIQLKIEQQALVKESDDASLKRLDSLNLELMQKEREYAELEEVWKAEKAALSGTQHIKTELE
TARSNMEIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEEVLHHRVIGQAEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELNYAGIKEVVMDVVSQHFRPEFLNRVDETVVFHPLAQEHIKNIASIQLQRLEKRLNEKDYQLQVTDEAL
NLIAEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRVGEVIKLKVKNEQLIATQNDDF

Sequences:

>Translated_861_residues
MRLDRFTSKFQMAISDAQSLALGQDHQYIEPTHLMVALLNQDGSTIRPLLTLLNVDVTQLRSKLTEILDKTPKVTGIGGD
VQLSSNMGVIFNLCDKVAQKRKDAYISSEIFMLAAIEDKGPLGNLLRELGLTEPKISKSIDEIRGGEKVNDQNAEEKRQA
LEKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DIGALVAGAKFRGEFEERLKAVLNELAKEEGSVILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTVAILRGLKERYEIHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLDRKIIQLKIEQQALVKESDDASLKRLDSLNLELMQKEREYAELEEVWKAEKAALSGTQHIKTELE
TARSNMEIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEEVLHHRVIGQAEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELNYAGIKEVVMDVVSQHFRPEFLNRVDETVVFHPLAQEHIKNIASIQLQRLEKRLNEKDYQLQVTDEAL
NLIAEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRVGEVIKLKVKNEQLIATQNDDF
>Mature_861_residues
MRLDRFTSKFQMAISDAQSLALGQDHQYIEPTHLMVALLNQDGSTIRPLLTLLNVDVTQLRSKLTEILDKTPKVTGIGGD
VQLSSNMGVIFNLCDKVAQKRKDAYISSEIFMLAAIEDKGPLGNLLRELGLTEPKISKSIDEIRGGEKVNDQNAEEKRQA
LEKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DIGALVAGAKFRGEFEERLKAVLNELAKEEGSVILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTVAILRGLKERYEIHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLDRKIIQLKIEQQALVKESDDASLKRLDSLNLELMQKEREYAELEEVWKAEKAALSGTQHIKTELE
TARSNMEIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEEVLHHRVIGQAEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELNYAGIKEVVMDVVSQHFRPEFLNRVDETVVFHPLAQEHIKNIASIQLQRLEKRLNEKDYQLQVTDEAL
NLIAEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRVGEVIKLKVKNEQLIATQNDDF

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=310, Percent_Identity=37.741935483871, Blast_Score=217, Evalue=3e-56,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=75.6126021003501, Blast_Score=1358, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=488, Percent_Identity=36.0655737704918, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=714, Percent_Identity=52.1008403361345, Blast_Score=764, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=863, Percent_Identity=42.5260718424102, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96327; Mature: 96327

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQMAISDAQSLALGQDHQYIEPTHLMVALLNQDGSTIRPLLTLLNVDVTQL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
RSKLTEILDKTPKVTGIGGDVQLSSNMGVIFNLCDKVAQKRKDAYISSEIFMLAAIEDKG
HHHHHHHHHCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGNLLRELGLTEPKISKSIDEIRGGEKVNDQNAEEKRQALEKFTVDLTERAEQGKLDPV
CHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DIGALVAGAKFRGEFEERLKAVLNELAKEEGSVILFIDEVHTMVGAGKGEGSMDAGNMLK
EHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTVAILRGLKERYEIH
HHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPESLDKLDRKII
HEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
QLKIEQQALVKESDDASLKRLDSLNLELMQKEREYAELEEVWKAEKAALSGTQHIKTELE
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TARSNMEIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRQTGIPVNKMLEGERDKLLKMEEVLHHRVIGQAEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSEKIQQHFGELNYAGIKEVVMDVVSQHFRPEFLNRVDETVVFHPLAQEHIKNIASIQLQ
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
RLEKRLNEKDYQLQVTDEALNLIAEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRV
HHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GEVIKLKVKNEQLIATQNDDF
CCEEEEEECCCEEEECCCCCC
>Mature Secondary Structure
MRLDRFTSKFQMAISDAQSLALGQDHQYIEPTHLMVALLNQDGSTIRPLLTLLNVDVTQL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
RSKLTEILDKTPKVTGIGGDVQLSSNMGVIFNLCDKVAQKRKDAYISSEIFMLAAIEDKG
HHHHHHHHHCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGNLLRELGLTEPKISKSIDEIRGGEKVNDQNAEEKRQALEKFTVDLTERAEQGKLDPV
CHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DIGALVAGAKFRGEFEERLKAVLNELAKEEGSVILFIDEVHTMVGAGKGEGSMDAGNMLK
EHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTVAILRGLKERYEIH
HHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPESLDKLDRKII
HEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
QLKIEQQALVKESDDASLKRLDSLNLELMQKEREYAELEEVWKAEKAALSGTQHIKTELE
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TARSNMEIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRQTGIPVNKMLEGERDKLLKMEEVLHHRVIGQAEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSEKIQQHFGELNYAGIKEVVMDVVSQHFRPEFLNRVDETVVFHPLAQEHIKNIASIQLQ
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
RLEKRLNEKDYQLQVTDEALNLIAEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRV
HHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GEVIKLKVKNEQLIATQNDDF
CCEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]