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The map label for this gene is trmD [H]

Identifier: 59711158

GI number: 59711158

Start: 599346

End: 600095

Strand: Direct

Name: trmD [H]

Synonym: VF_0551

Alternate gene names: 59711158

Gene position: 599346-600095 (Clockwise)

Preceding gene: 59711157

Following gene: 59711159

Centisome position: 20.68

GC content: 43.87

Gene sequence:

>750_bases
ATGTGGGTTGGCGTTATTAGCCTATTTCCTGAAATGTTCCGTTCGGTTACCGATTTTGGGGTAACTAGCCAAGCAATAAA
AAAAGGTCTTTTATCTATTGAGACATGGAATCCTAGAGATTTCACTCATGATAAACATCGCACTGTTGATGACCGACCTT
ATGGTGGTGGTCCTGGAATGTTAATGATGGTACAGCCTTTGCGCGATGCCATTACTGCAGCCAGAGAAGCGTCACCCGGC
AAAACGAAAGTGATTTACCTTTCACCTCAAGGCAGAACGCTGAATCAAGCTGGTGTTGAGGAGTTAGCGACAAACGAGAA
TTTAATTCTAATTTGTGGCCGATACGAAGGTGTAGATGAGCGCATAATACAATCTGAAGTTGACGAAGAATGGTCAATTG
GGGATTTTGTGCTTACAGGTGGGGAACTCCCTGCTATGACGTTGATTGATTCTGTATCTCGGTTTGTTCCGGGTGTATTA
GGTGATTTCGCGTCCGCTGAAGAAGATTCTTTTGCAGACGGTTTGTTAGATTGTCCGCACTATACAAGACCTGAGGTTTT
GGATGGTAAGGAAGTACCTAGTGTACTTAAATCTGGAAACCATAAAGATATCGCTCGTTGGCGAATGAAACAATCGTTAG
GCCGTACTTGGCTAAGAAGACCAGAGCTCCTGGGAAATCTAGCTCTGACTGACGAACAGGAATTATTACTGGCTGAGTTT
GTTCGTGAAGAACGCCAGAACAGTAAGTAA

Upstream 100 bases:

>100_bases
TTTATTGATGAGCAAGTGATCAAATTAATTGATCGTGAAGCTCAACGGATCGAAGTTGACTGGGATCCTGGGTTTTAACC
CCTAGGTAAAGCGAGGGCAC

Downstream 100 bases:

>100_bases
TTAAGTAAATTTAGTATCAGTTTATTCTAGGATATACAAAAATGAGCAACATCATTAAAGCTCTTGAAGATGAGCAACTA
AAACAAGACCTACCTAAATT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREASPG
KTKVIYLSPQGRTLNQAGVEELATNENLILICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREERQNSK

Sequences:

>Translated_249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREASPG
KTKVIYLSPQGRTLNQAGVEELATNENLILICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREERQNSK
>Mature_249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREASPG
KTKVIYLSPQGRTLNQAGVEELATNENLILICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREERQNSK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=249, Percent_Identity=77.1084337349398, Blast_Score=399, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27833; Mature: 27833

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAITAAREASPGKTKVIYLSPQGRTLNQAGVEELATNENLILICGRYEGVDE
EEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFADGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
YTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHH
VREERQNSK
HHHHHHCCC
>Mature Secondary Structure
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAITAAREASPGKTKVIYLSPQGRTLNQAGVEELATNENLILICGRYEGVDE
EEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFADGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
YTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHH
VREERQNSK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA