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The map label for this gene is gshA

Identifier: 59711150

GI number: 59711150

Start: 591662

End: 593227

Strand: Direct

Name: gshA

Synonym: VF_0543

Alternate gene names: 59711150

Gene position: 591662-593227 (Clockwise)

Preceding gene: 59711149

Following gene: 59711151

Centisome position: 20.42

GC content: 38.06

Gene sequence:

>1566_bases
TTGACCGAATTTATCAACCGATTACAGAAAGTCGCTTCCAATCCTAACGCCTTTAAAAAAACAGGCCGAGGAATTGAAAG
AGAAACGCTTCGATTCACTCTAGGAGCGTCACTTTCAACTAAGCCACACCCAGAAGGCGTAGGTTCAGCATTAACACATA
AATACATCACAACAGATTTTGCAGAATCATTATTAGAGTTTATTACTCCAGTATCAAATGATGTTGATACCGTATTAAAG
CAATTAGAAGATGTACACCACTATACGGTTTCTCACATGGGAGACGAAAAACTATGGCCACTTTCAATGCCATGTTTTGT
TACTCATGATGACGATATTACGTTAGCTCAATACGGTGAATCGAACGTAGGTAAACTGAAAACAACTTATCGTGAAGGCC
TAAAGCGTCGTTACGGTAGCGTGATGCAAGTGATTTCTGGCGTACATTTTAATTTTTCATTTTCAACAGAGTTTTGGGAT
GAATTATTTGGTGAACAATCAGAAGACCAAAGAAAAGAATCGGTATCAGATGCTTATTTTGCATTAATTCGAAACTACTA
CCGTTTTGGTTGGTTAATTCCTTATTTCTTTGGTGCTTCACCGGCATTATGTAGCTCATTTATTCAAGGTCGTGAAACCT
CTATGGACTTTGAATCCTTAGGTAAGACTTATTACTTACCTTATGCTACATCGTTGCGTCTGAGTGATTTGGGCTATACC
AATGATGCTCAAAGTAATCTAAAAATTAGCCTGAACAGCGTTAATGAATATGTTGATGGTTTAAATAAAGCCATTCGAAC
ACCATCAGAAGAGTTCGCTAAAATAGGTTTAAAAGAAGGTGATAAACATATTCAGTTGAACTCAAATGTTCTTCAAATTG
AAAATGAACTTTATGCTCCAATTCGCCCAAAACGAGTGACAAAAAGTGGCGAAAGACCTTCTGAAGCACTAGAAAGAGAC
GGGGTTGAATACATTGAAGTTCGTTCTTTAGATGTAAACCCGTACAGTCCAATTGGTGTTAATGAAGATCAAGTTCGCTT
CTTAGATATGTTCTTAACTTGGACTGTTCTGAGTGACTCTGCACCAATGAATGACAGTGAAATGGCCTGTTGGAAAGACA
ACTGGAATAAGATCATTGAAAAAGGTCGTAAGCCGGGGTTAGAACTAAAAATTGGTTGCCAAGGTGAACGATTAACTCAA
AAAGCATGGGCTGAAAGAGTATTTGAAGATTTGCTGGTTATCGCAAAAGAAATGGATCGCGTTAACGGTGATGACGCTTA
TCAACAGACTCATAAACGCCTAAGTGCAATGATTGATGATCCTGAGCTAACGATTTCAGGTCGTCTATTGGCTGAAACAA
AAGCCGCTGGCGGTATCGGTGTGATTGGTTGTAAATTGGCGGTAGAGCATCGTGAAACACACTTAGCTCATCAATATCGT
TTTTATACTAAGCAAGAGTTAGATGCTGAAGTTGAACGTTCAGTTCAGGCACAAAAAGAAATTGAAGCGAATGATAAGCT
TTCTTTTTCTGAATATTTAGAAGAATACTTCAGTTATTTGAAGTGA

Upstream 100 bases:

>100_bases
TTATTGCCGTTTTTTCGTATCCAACTATCGATAAAATTTGTTAGTATCCATGACATAAATCAACATAATAATGTTGTTAC
CTCAATAGCGAGATTGTATT

Downstream 100 bases:

>100_bases
TACTTACGAAGAAATATCTAGCACTGTTATTCTATGGGTAGCAGTGCTTTTTTTTGAGAAAAATTGATGAAATATAAACT
TCCACTTGCCGCTATAGCAT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTEFINRLQKVASNPNAFKKTGRGIERETLRFTLGASLSTKPHPEGVGSALTHKYITTDFAESLLEFITPVSNDVDTVLK
QLEDVHHYTVSHMGDEKLWPLSMPCFVTHDDDITLAQYGESNVGKLKTTYREGLKRRYGSVMQVISGVHFNFSFSTEFWD
ELFGEQSEDQRKESVSDAYFALIRNYYRFGWLIPYFFGASPALCSSFIQGRETSMDFESLGKTYYLPYATSLRLSDLGYT
NDAQSNLKISLNSVNEYVDGLNKAIRTPSEEFAKIGLKEGDKHIQLNSNVLQIENELYAPIRPKRVTKSGERPSEALERD
GVEYIEVRSLDVNPYSPIGVNEDQVRFLDMFLTWTVLSDSAPMNDSEMACWKDNWNKIIEKGRKPGLELKIGCQGERLTQ
KAWAERVFEDLLVIAKEMDRVNGDDAYQQTHKRLSAMIDDPELTISGRLLAETKAAGGIGVIGCKLAVEHRETHLAHQYR
FYTKQELDAEVERSVQAQKEIEANDKLSFSEYLEEYFSYLK

Sequences:

>Translated_521_residues
MTEFINRLQKVASNPNAFKKTGRGIERETLRFTLGASLSTKPHPEGVGSALTHKYITTDFAESLLEFITPVSNDVDTVLK
QLEDVHHYTVSHMGDEKLWPLSMPCFVTHDDDITLAQYGESNVGKLKTTYREGLKRRYGSVMQVISGVHFNFSFSTEFWD
ELFGEQSEDQRKESVSDAYFALIRNYYRFGWLIPYFFGASPALCSSFIQGRETSMDFESLGKTYYLPYATSLRLSDLGYT
NDAQSNLKISLNSVNEYVDGLNKAIRTPSEEFAKIGLKEGDKHIQLNSNVLQIENELYAPIRPKRVTKSGERPSEALERD
GVEYIEVRSLDVNPYSPIGVNEDQVRFLDMFLTWTVLSDSAPMNDSEMACWKDNWNKIIEKGRKPGLELKIGCQGERLTQ
KAWAERVFEDLLVIAKEMDRVNGDDAYQQTHKRLSAMIDDPELTISGRLLAETKAAGGIGVIGCKLAVEHRETHLAHQYR
FYTKQELDAEVERSVQAQKEIEANDKLSFSEYLEEYFSYLK
>Mature_520_residues
TEFINRLQKVASNPNAFKKTGRGIERETLRFTLGASLSTKPHPEGVGSALTHKYITTDFAESLLEFITPVSNDVDTVLKQ
LEDVHHYTVSHMGDEKLWPLSMPCFVTHDDDITLAQYGESNVGKLKTTYREGLKRRYGSVMQVISGVHFNFSFSTEFWDE
LFGEQSEDQRKESVSDAYFALIRNYYRFGWLIPYFFGASPALCSSFIQGRETSMDFESLGKTYYLPYATSLRLSDLGYTN
DAQSNLKISLNSVNEYVDGLNKAIRTPSEEFAKIGLKEGDKHIQLNSNVLQIENELYAPIRPKRVTKSGERPSEALERDG
VEYIEVRSLDVNPYSPIGVNEDQVRFLDMFLTWTVLSDSAPMNDSEMACWKDNWNKIIEKGRKPGLELKIGCQGERLTQK
AWAERVFEDLLVIAKEMDRVNGDDAYQQTHKRLSAMIDDPELTISGRLLAETKAAGGIGVIGCKLAVEHRETHLAHQYRF
YTKQELDAEVERSVQAQKEIEANDKLSFSEYLEEYFSYLK

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=504, Percent_Identity=55.1587301587302, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_VIBF1 (Q5E7F8)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203926.1
- ProteinModelPortal:   Q5E7F8
- SMR:   Q5E7F8
- STRING:   Q5E7F8
- GeneID:   3278110
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0543
- NMPDR:   fig|312309.3.peg.593
- eggNOG:   COG2918
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- PhylomeDB:   Q5E7F8
- ProtClustDB:   PRK02107
- BioCyc:   VFIS312309:VF0543-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 59405; Mature: 59274

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFINRLQKVASNPNAFKKTGRGIERETLRFTLGASLSTKPHPEGVGSALTHKYITTDF
CHHHHHHHHHHHCCCHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
AESLLEFITPVSNDVDTVLKQLEDVHHYTVSHMGDEKLWPLSMPCFVTHDDDITLAQYGE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEHHHCCC
SNVGKLKTTYREGLKRRYGSVMQVISGVHFNFSFSTEFWDELFGEQSEDQRKESVSDAYF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHH
ALIRNYYRFGWLIPYFFGASPALCSSFIQGRETSMDFESLGKTYYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NDAQSNLKISLNSVNEYVDGLNKAIRTPSEEFAKIGLKEGDKHIQLNSNVLQIENELYAP
CCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHCCC
IRPKRVTKSGERPSEALERDGVEYIEVRSLDVNPYSPIGVNEDQVRFLDMFLTWTVLSDS
CCCHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
APMNDSEMACWKDNWNKIIEKGRKPGLELKIGCQGERLTQKAWAERVFEDLLVIAKEMDR
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VNGDDAYQQTHKRLSAMIDDPELTISGRLLAETKAAGGIGVIGCKLAVEHRETHLAHQYR
CCCCHHHHHHHHHHHHHCCCCCEEECCEEEEHHCCCCCCEEEEEEEEHHHHHHHHHHHHH
FYTKQELDAEVERSVQAQKEIEANDKLSFSEYLEEYFSYLK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFINRLQKVASNPNAFKKTGRGIERETLRFTLGASLSTKPHPEGVGSALTHKYITTDF
HHHHHHHHHHHCCCHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
AESLLEFITPVSNDVDTVLKQLEDVHHYTVSHMGDEKLWPLSMPCFVTHDDDITLAQYGE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEHHHCCC
SNVGKLKTTYREGLKRRYGSVMQVISGVHFNFSFSTEFWDELFGEQSEDQRKESVSDAYF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHH
ALIRNYYRFGWLIPYFFGASPALCSSFIQGRETSMDFESLGKTYYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NDAQSNLKISLNSVNEYVDGLNKAIRTPSEEFAKIGLKEGDKHIQLNSNVLQIENELYAP
CCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHCCC
IRPKRVTKSGERPSEALERDGVEYIEVRSLDVNPYSPIGVNEDQVRFLDMFLTWTVLSDS
CCCHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
APMNDSEMACWKDNWNKIIEKGRKPGLELKIGCQGERLTQKAWAERVFEDLLVIAKEMDR
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VNGDDAYQQTHKRLSAMIDDPELTISGRLLAETKAAGGIGVIGCKLAVEHRETHLAHQYR
CCCCHHHHHHHHHHHHHCCCCCEEECCEEEEHHCCCCCCEEEEEEEEHHHHHHHHHHHHH
FYTKQELDAEVERSVQAQKEIEANDKLSFSEYLEEYFSYLK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA