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The map label for this gene is prfC [H]

Identifier: 59711107

GI number: 59711107

Start: 536476

End: 538056

Strand: Direct

Name: prfC [H]

Synonym: VF_0500

Alternate gene names: 59711107

Gene position: 536476-538056 (Clockwise)

Preceding gene: 59711106

Following gene: 172087667

Centisome position: 18.51

GC content: 42.63

Gene sequence:

>1581_bases
ATGTCTTTTTTACAAGAAGTGGGTAAACGCAGAACGTTTGCCATCATTTCTCACCCGGATGCGGGTAAAACAACCATTAC
TGAAAAAGTATTATTATTCGGAAACGCGATTCAAAAAGCGGGTACCGTAAAAGGTCGTGGTTCAAACCAGCACGCAAAAT
CAGACTGGATGGAAATGGAAAAAGAACGTGGTATCTCTGTTACTACGTCAGTAATGCAATTCCCATTCAACGATTGCTTA
GTTAACTTACTTGATACTCCTGGACACGAAGATTTCTCGGAAGATACTTATCGTACATTAACTGCTGTTGACTCATGCCT
AATGGTAATTGATGCAGCAAAAGGTGTCGAAGACCGTACTCGTAAATTAATGGAAGTGACACGTCTACGTGATACGCCAA
TCGTAACCTTCATGAACAAATTAGACCGTGAAGTTCGTGACCCAATGGAAGTGCTTGATGAAGTAGAAAGCGAGTTAGGT
ATGGCTTGTGCACCTATTTCATGGCCTATTGGTTGTGGTAAAGAGTTTAAAGGTGTTTACCATATTCACCGTGACGAAAC
GATTTTATATGAATCAGGTCATGGTCATGAAATCCAAGAAGTTCGTACGATCAAAGGTCTTGATAACCCTGAACTGGATG
CAACAGTTGGTGCAGATTTGGCTGAAAGTGTTCGTGAAGAGTTAGAACTTGTTATGGGCGCATGTCCTGAGTTTGATCAT
GAGCTTTTCCTAGCTGGTGAGCTAACGCCAGTGTACTTTGGTACTGCTCTTGGTAACTTTGGTGTTGACCATATGCTAGA
AGGCTTAACGGATTGGGCTCCTGCGCCACAAACTCGTCAAGCAAATGAGCGTGATGTTGTTGCAACAGAAGATAAGTTCT
CAGGCTTTGTTTTTAAGATCCAAGCAAACATGGATCCTAAACACCGCGACCGTATTGCCTTTATGCGTATTGTATCTGGT
ACATACACTCAAGGCATGAAGATGAACCATGTTCGTACAGGCAAAAACGTCAGTATTTCTGATGCGGTAACCTTTATGGC
GGGTGACCGTTCACGTGCTGAAACGGCTTATGCTGGTGACATTATCGGCCTACATAACCATGGTACTATTCAAATTGGTG
ATACGTTTACTCAAGGTGAAAGCTTGAAGTTCTCAGGTATTCCTAACTTTGCACCAGAATTGTTCCGTCGTATCCGTTTA
AAAGATCCTCTAAAGCAAAAACAATTGCTTAAAGGTCTAGTTCAATTATCTGAAGAAGGTGCGGTACAAGTATTCCGTCC
ACTGCAAAACAACGATCTTATTGTTGGTGCGGTTGGTGTTCTTCAGTTCGACGTAGTTGTTGCTCGTCTTAAAGCAGAAT
ACAACGTTGAAGCCATCTATGAAGGTGTAAGTGTTGCAACGGCTCGTTGGGTTGATTGTGCAGATGGTAAGAAGATGGAT
GAGTTCCAACGTAAGAACCAAGCTAACTTGGCGCTTGATGGTGGTGACAACCTAACTTACATCGCACCGACAATGGTTAA
TCTAAACCTTGCTTCTGAACGTTTCCCTGATGTTCAATTTAGAGCGACGCGTGAGCACTAA

Upstream 100 bases:

>100_bases
CTAAATTCCTTACTTTAGATAAGAGGCGAGTTTGCGTATAATCTCGCCTAATTATTACATGTTCTATCCTTTCTGAAATC
TTACAGGATTATCAACGCTT

Downstream 100 bases:

>100_bases
TTAATCAGTTGCTTATCAAATAAATAAAAACGGAGTCTTAACGGACTCCGTTTTTGTATCTATAGTAATAGCAATGCACT
TATACCCATCAACTCATTCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNDCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRTIKGLDNPELDATVGADLAESVREELELVMGACPEFDH
ELFLAGELTPVYFGTALGNFGVDHMLEGLTDWAPAPQTRQANERDVVATEDKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYTQGMKMNHVRTGKNVSISDAVTFMAGDRSRAETAYAGDIIGLHNHGTIQIGDTFTQGESLKFSGIPNFAPELFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVSVATARWVDCADGKKMD
EFQRKNQANLALDGGDNLTYIAPTMVNLNLASERFPDVQFRATREH

Sequences:

>Translated_526_residues
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNDCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRTIKGLDNPELDATVGADLAESVREELELVMGACPEFDH
ELFLAGELTPVYFGTALGNFGVDHMLEGLTDWAPAPQTRQANERDVVATEDKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYTQGMKMNHVRTGKNVSISDAVTFMAGDRSRAETAYAGDIIGLHNHGTIQIGDTFTQGESLKFSGIPNFAPELFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVSVATARWVDCADGKKMD
EFQRKNQANLALDGGDNLTYIAPTMVNLNLASERFPDVQFRATREH
>Mature_525_residues
SFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNDCLV
NLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREVRDPMEVLDEVESELGM
ACAPISWPIGCGKEFKGVYHIHRDETILYESGHGHEIQEVRTIKGLDNPELDATVGADLAESVREELELVMGACPEFDHE
LFLAGELTPVYFGTALGNFGVDHMLEGLTDWAPAPQTRQANERDVVATEDKFSGFVFKIQANMDPKHRDRIAFMRIVSGT
YTQGMKMNHVRTGKNVSISDAVTFMAGDRSRAETAYAGDIIGLHNHGTIQIGDTFTQGESLKFSGIPNFAPELFRRIRLK
DPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVSVATARWVDCADGKKMDE
FQRKNQANLALDGGDNLTYIAPTMVNLNLASERFPDVQFRATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=479, Percent_Identity=27.1398747390397, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.078431372549, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.1725888324873, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=24.4813278008299, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI157426893, Length=173, Percent_Identity=30.635838150289, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.9270072992701, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=81.6793893129771, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=500, Percent_Identity=26.4, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=36.241610738255, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=529, Percent_Identity=26.0869565217391, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=32.4022346368715, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=31.3432835820896, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=29.3233082706767, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=29.3233082706767, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=27.1966527196653, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=422, Percent_Identity=25.5924170616114, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.1655629139073, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=28.099173553719, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=24.3564356435644, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=28.1045751633987, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=28.1045751633987, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=28.1045751633987, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58609; Mature: 58478

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPFNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKLDREVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYESGHGHEIQEVRTIKGLDNPELDATVGADLAESVREELELVMGACPEFDH
EEECCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCC
ELFLAGELTPVYFGTALGNFGVDHMLEGLTDWAPAPQTRQANERDVVATEDKFSGFVFKI
CEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEE
QANMDPKHRDRIAFMRIVSGTYTQGMKMNHVRTGKNVSISDAVTFMAGDRSRAETAYAGD
ECCCCCCHHHHHHHHHHHCCCHHCCEEECEECCCCCCCHHHHEEEEECCCCCCCHHHCCC
IIGLHNHGTIQIGDTFTQGESLKFSGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVSVATARWVDCADGKKMD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHEEECCCCCCHH
EFQRKNQANLALDGGDNLTYIAPTMVNLNLASERFPDVQFRATREH
HHHHCCCCCEEEECCCCEEEEECEEEEEEECCCCCCCCEEEECCCC
>Mature Secondary Structure 
SFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPFNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKLDREVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYESGHGHEIQEVRTIKGLDNPELDATVGADLAESVREELELVMGACPEFDH
EEECCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCC
ELFLAGELTPVYFGTALGNFGVDHMLEGLTDWAPAPQTRQANERDVVATEDKFSGFVFKI
CEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEE
QANMDPKHRDRIAFMRIVSGTYTQGMKMNHVRTGKNVSISDAVTFMAGDRSRAETAYAGD
ECCCCCCHHHHHHHHHHHCCCHHCCEEECEECCCCCCCHHHHEEEEECCCCCCCHHHCCC
IIGLHNHGTIQIGDTFTQGESLKFSGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVARLKAEYNVEAIYEGVSVATARWVDCADGKKMD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHEEECCCCCCHH
EFQRKNQANLALDGGDNLTYIAPTMVNLNLASERFPDVQFRATREH
HHHHCCCCCEEEECCCCEEEEECEEEEEEECCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA