Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 59711038

GI number: 59711038

Start: 458938

End: 459975

Strand: Direct

Name: pilT [H]

Synonym: VF_0431

Alternate gene names: 59711038

Gene position: 458938-459975 (Clockwise)

Preceding gene: 59711030

Following gene: 59711039

Centisome position: 15.84

GC content: 39.98

Gene sequence:

>1038_bases
ATGGACATCACAGAACTACTGGACTTTAGTGTAAAACAAAACGCATCAGATCTACACCTATCTGCAGGAGTTCCTCCTAT
GGTACGTGTAGATGGTGAAGTGCGTAAACTGACATTACCTGATTTTACACATGCCGATGTTCATCGTTTAGTGACTGACA
TTATGAATGATGCTCAGCGCAGTGAATTTGAAGATAAACTAGAAGTCGATTTTTCATTTGAGTTACCTAATATTGGACGC
TTTCGTGTAAATGCATTTAACCAGTCTCGTGGCTGTGCTGCTGTGTTTCGAACTATCCCTGTTGATATTCCGTCATTAGA
GCAATTAGCTGCACCTCAAGTGTTCACCGATATTGCAAAATATCAAAAAGGATTAGTGTTAATCACAGGGCCTACTGGTT
CAGGTAAGTCGACAACCTTAGCTGCTATGGTTGATTACATTAACGAGAACTACAACAAGCACGTACTTACGATTGAAGAT
CCGATTGAATTTGTTCATAAGAATAAGAAGTGTTTGATTAACCAACGTGAAGTTAATCGTGATACACATAGCTTTAAAGC
AGCACTTCGCTCAGCTTTGCGTGAAGATCCAGATGTTATTTTAGTCGGTGAATTACGTGATCAAGAGACAATCAGTCTTG
CATTGACCGCTGCTGAAACTGGGCATTTAGTATTTGGTACTTTGCACACGAGTTCAGCTGCAAAAACCATTGACCGTATT
ATTGATGTTTTCCCTGGCAGTGATAAATCTATGGTTCGCTCGATGTTGTCTGAATCATTAAGAGCGGTAGTAGCACAAAC
ACTGCTTAAACGTATTGGTGGTGGTCGTATTGCGTGTCATGAAATCATGATAGCGACACCAGCTATCCGTAACTTAATCC
GTGAAGATAAAGTCGCTCAAATGTATTCAATCATTCAAACTGGTTCTTCTTTTGGTATGAATACCATGGAGCAGAATGCG
AAGAAAATTATTGCTCAAGGTTTGGTTGATCCGGATGAAGTCGTTAAGAAAGTTGAAATGAGCAGCATAGGGTTTTAG

Upstream 100 bases:

>100_bases
GGGTTTCGACCACATTTTTGAATGCTATTCTCTATTTGATGAGTGATTTGATTGATATTTTGCTTAATACTAGTCATAGA
AACGATTACCGAGGAAAATT

Downstream 100 bases:

>100_bases
TCATGACATTGGATCAAATACTGAAACAAATGATCACTTTAAAAGCATCGGATATTTACTTAACGGTTGATGCTCCTTGC
TTGCTGAGAGTGAATGGTGA

Product: transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQRSEFEDKLEVDFSFELPNIGR
FRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAKYQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIED
PIEFVHKNKKCLINQREVNRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMNTMEQNA
KKIIAQGLVDPDEVVKKVEMSSIGF

Sequences:

>Translated_345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQRSEFEDKLEVDFSFELPNIGR
FRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAKYQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIED
PIEFVHKNKKCLINQREVNRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMNTMEQNA
KKIIAQGLVDPDEVVKKVEMSSIGF
>Mature_345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQRSEFEDKLEVDFSFELPNIGR
FRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAKYQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIED
PIEFVHKNKKCLINQREVNRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMNTMEQNA
KKIIAQGLVDPDEVVKKVEMSSIGF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=46.4831804281346, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=32.0916905444126, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786296, Length=391, Percent_Identity=29.156010230179, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38137; Mature: 38137

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SEFEDKLEVDFSFELPNIGRFRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAK
HCCCHHEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHCHHHHHHHHH
YQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIEDPIEFVHKNKKCLINQREVNR
HHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHEECHHHCCC
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHH
MYSIIQTGSSFGMNTMEQNAKKIIAQGLVDPDEVVKKVEMSSIGF
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SEFEDKLEVDFSFELPNIGRFRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAK
HCCCHHEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHCHHHHHHHHH
YQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIEDPIEFVHKNKKCLINQREVNR
HHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHEECHHHCCC
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHH
MYSIIQTGSSFGMNTMEQNAKKIIAQGLVDPDEVVKKVEMSSIGF
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]