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The map label for this gene is holC [H]

Identifier: 59711019

GI number: 59711019

Start: 442324

End: 442767

Strand: Reverse

Name: holC [H]

Synonym: VF_0412

Alternate gene names: 59711019

Gene position: 442767-442324 (Counterclockwise)

Preceding gene: 59711020

Following gene: 59711018

Centisome position: 15.28

GC content: 42.12

Gene sequence:

>444_bases
ATGAGCCAAGCCCTGTTTTATGTTTTAGAACCCACATCTCCAGCTGCAACCACTGATGGTCAGCTAAAATTTGTGTGTCA
GCTTGCTCACTATTATTACTCTCAAAATGCACGAGTGTATGTATTAGCCCAAGATAAAGAACAAGCCTTGCTGATTGATG
AAGCACTTTGGCAATATGATGTATCAGAATTTACGCCACACAATTTAGTTGGAGAAGGGCCAAAATCAGGCTCCCCTATC
GAAATTGGTTGGGGAACATTACGCTCATCAGGACGCCGTCATGTGTTAATTAATTTAGCTCAAGAGGTCGCAAGTTTTGC
TGTTTCCTTTGCTCAAGTGGTAGACTTCGTACCTTGCGAAGAAAAAAGCAAACAACAAGCTCGAGAAAGGTATAAAATAT
ACCGAAATGCAGGCCGAGCCATGCAAACCGAGATACTGAAATGA

Upstream 100 bases:

>100_bases
GGCCAAGAAAACGAAGAATAAACCAATTCGTTTTATGATATAAAGGGCCTATATTGAGGCCCTTTTTTAATACGTACTAT
TTATCCACTTAAGAGAGTTT

Downstream 100 bases:

>100_bases
TGCCAATCATCATAGCTAAATGACTCATATATTTATTCTTTTTAATAATGAGCCATTAATTATGATATTTGGCATTCCAC
TTTTTAATAGAGATATTCGT

Product: DNA polymerase III subunit chi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSQALFYVLEPTSPAATTDGQLKFVCQLAHYYYSQNARVYVLAQDKEQALLIDEALWQYDVSEFTPHNLVGEGPKSGSPI
EIGWGTLRSSGRRHVLINLAQEVASFAVSFAQVVDFVPCEEKSKQQARERYKIYRNAGRAMQTEILK

Sequences:

>Translated_147_residues
MSQALFYVLEPTSPAATTDGQLKFVCQLAHYYYSQNARVYVLAQDKEQALLIDEALWQYDVSEFTPHNLVGEGPKSGSPI
EIGWGTLRSSGRRHVLINLAQEVASFAVSFAQVVDFVPCEEKSKQQARERYKIYRNAGRAMQTEILK
>Mature_146_residues
SQALFYVLEPTSPAATTDGQLKFVCQLAHYYYSQNARVYVLAQDKEQALLIDEALWQYDVSEFTPHNLVGEGPKSGSPIE
IGWGTLRSSGRRHVLINLAQEVASFAVSFAQVVDFVPCEEKSKQQARERYKIYRNAGRAMQTEILK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=147, Percent_Identity=44.2176870748299, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007459 [H]

Pfam domain/function: PF04364 DNA_pol3_chi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16605; Mature: 16474

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALFYVLEPTSPAATTDGQLKFVCQLAHYYYSQNARVYVLAQDKEQALLIDEALWQYD
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHC
VSEFTPHNLVGEGPKSGSPIEIGWGTLRSSGRRHVLINLAQEVASFAVSFAQVVDFVPCE
HHHCCCCCCCCCCCCCCCCEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
EKSKQQARERYKIYRNAGRAMQTEILK
CHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SQALFYVLEPTSPAATTDGQLKFVCQLAHYYYSQNARVYVLAQDKEQALLIDEALWQYD
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHC
VSEFTPHNLVGEGPKSGSPIEIGWGTLRSSGRRHVLINLAQEVASFAVSFAQVVDFVPCE
HHHCCCCCCCCCCCCCCCCEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
EKSKQQARERYKIYRNAGRAMQTEILK
CHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8389364; 8246893; 7610040; 9278503; 2670557; 8505305; 1575709 [H]