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The map label for this gene is argI

Identifier: 59711014

GI number: 59711014

Start: 435399

End: 436403

Strand: Reverse

Name: argI

Synonym: VF_0407

Alternate gene names: 59711014

Gene position: 436403-435399 (Counterclockwise)

Preceding gene: 59711015

Following gene: 172087660

Centisome position: 15.06

GC content: 39.7

Gene sequence:

>1005_bases
ATGGCTTTTAATTTACGTAATAGAAACTTTTTAAAACTATTAGATTTCACCCCTAAAGAGATCGCACATCTACTTGAGTT
ATCTGCTGAGCTGAAAAAAGCAAAGTACTCAGGTTATGAACAACCTCGCTTAACAGGGAAAAACATTGCGTTAATTTTTG
AGAAGTCATCAACACGTACTCGCTGTGCCTTTGAAGTTGCAGCCTTTGATCAAGGAGCTAAAGTCACTTACCTTGGACCT
TCTGGATCCCAAATTGGCCATAAGGAATCCATGAAGGATACCGCCCGTGTATTAGGCCGCATGTATGATGGCATTGAATA
TCGTGGTTTTGGTCAAACGATTGTTGAAGAATTAGGTCAATATGCAGGAGTCCCTGTATGGAACGGATTAACGGATGAAT
TTCATCCAACTCAAATCTTGGCTGATTTTCTAACAATGCAAGAATATGCAAATGGAAAGCAACTTAGCCAAATTACGTTT
GCCTATCTTGGTGATGCTCGAAACAATATGGGTAATTCTCTAATGGTTGGAGCAGCAAAAATGGGAATGGAAATTCGCCT
TGTTGCACCAAAACAGTTCTGGCCAGAAGAAGAGTTGGTTACTCAATGCCAAGAAATTGCACTACAAACTGGTGCAAAAA
TTGTGTTAACTGAAGAAGTTACAGAAGGCGTTAAAGGTTGTGACTTCCTTTATACCGATGTATGGGTCTCAATGGGTGAA
GCACCTGAAGCATGGGATGAGCGCGTTGCTTTAATGACCCCTTATCAGATCAATATGGATGTTATCAAAGCAACTGAAAA
TCCTAATGTGAAATTCATGCACTGCTTACCTGCTTTTCATAATGATGAAACAACACTTGGTAAGGAGATTGCCGAGAAGT
ACGGTATGAATGGCTTAGAGGTCACAGAAGATGTCTTTGAATCTGAATATTCCATTGTTTTTGATGAAGCTGAAAACCGT
ATGCACACAATTAAAGCGGTTATGGTTGCAACACTTGGTAGTTAA

Upstream 100 bases:

>100_bases
AACATATACACATTAAATAAATCAAAATAAATATTCACAACTAATGAATTTTTATGTATAACTATAAATCACTGGATTGA
TTTTTGGCAAGGAGCACATG

Downstream 100 bases:

>100_bases
TTGAATACAAATTTTTGATGCAAACGCTTGCTTAGGAATTTGATACGAGTATAATTCCGCCCAATTTATTAGGGAATTAT
GATGTTATTAACTTATCTTC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNLRNRNFLKLLDFTPKEIAHLLELSAELKKAKYSGYEQPRLTGKNIALIFEKSSTRTRCAFEVAAFDQGAKVTYLGP
SGSQIGHKESMKDTARVLGRMYDGIEYRGFGQTIVEELGQYAGVPVWNGLTDEFHPTQILADFLTMQEYANGKQLSQITF
AYLGDARNNMGNSLMVGAAKMGMEIRLVAPKQFWPEEELVTQCQEIALQTGAKIVLTEEVTEGVKGCDFLYTDVWVSMGE
APEAWDERVALMTPYQINMDVIKATENPNVKFMHCLPAFHNDETTLGKEIAEKYGMNGLEVTEDVFESEYSIVFDEAENR
MHTIKAVMVATLGS

Sequences:

>Translated_334_residues
MAFNLRNRNFLKLLDFTPKEIAHLLELSAELKKAKYSGYEQPRLTGKNIALIFEKSSTRTRCAFEVAAFDQGAKVTYLGP
SGSQIGHKESMKDTARVLGRMYDGIEYRGFGQTIVEELGQYAGVPVWNGLTDEFHPTQILADFLTMQEYANGKQLSQITF
AYLGDARNNMGNSLMVGAAKMGMEIRLVAPKQFWPEEELVTQCQEIALQTGAKIVLTEEVTEGVKGCDFLYTDVWVSMGE
APEAWDERVALMTPYQINMDVIKATENPNVKFMHCLPAFHNDETTLGKEIAEKYGMNGLEVTEDVFESEYSIVFDEAENR
MHTIKAVMVATLGS
>Mature_333_residues
AFNLRNRNFLKLLDFTPKEIAHLLELSAELKKAKYSGYEQPRLTGKNIALIFEKSSTRTRCAFEVAAFDQGAKVTYLGPS
GSQIGHKESMKDTARVLGRMYDGIEYRGFGQTIVEELGQYAGVPVWNGLTDEFHPTQILADFLTMQEYANGKQLSQITFA
YLGDARNNMGNSLMVGAAKMGMEIRLVAPKQFWPEEELVTQCQEIALQTGAKIVLTEEVTEGVKGCDFLYTDVWVSMGEA
PEAWDERVALMTPYQINMDVIKATENPNVKFMHCLPAFHNDETTLGKEIAEKYGMNGLEVTEDVFESEYSIVFDEAENRM
HTIKAVMVATLGS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=329, Percent_Identity=37.6899696048632, Blast_Score=208, Evalue=5e-54,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=68.1957186544342, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=67.3716012084592, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI48994908, Length=378, Percent_Identity=23.2804232804233, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=32.6219512195122, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322331, Length=276, Percent_Identity=26.8115942028986, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_VIBF1 (Q5E7U4)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203790.1
- HSSP:   P04391
- ProteinModelPortal:   Q5E7U4
- SMR:   Q5E7U4
- STRING:   Q5E7U4
- GeneID:   3277308
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0407
- NMPDR:   fig|312309.3.peg.407
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- PhylomeDB:   Q5E7U4
- ProtClustDB:   PRK02102
- BioCyc:   VFIS312309:VF0407-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 37438; Mature: 37307

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLRNRNFLKLLDFTPKEIAHLLELSAELKKAKYSGYEQPRLTGKNIALIFEKSSTRT
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
RCAFEVAAFDQGAKVTYLGPSGSQIGHKESMKDTARVLGRMYDGIEYRGFGQTIVEELGQ
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADFLTMQEYANGKQLSQITFAYLGDARNNMGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCEEEEEHHH
MGMEIRLVAPKQFWPEEELVTQCQEIALQTGAKIVLTEEVTEGVKGCDFLYTDVWVSMGE
CCEEEEEECCHHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCC
APEAWDERVALMTPYQINMDVIKATENPNVKFMHCLPAFHNDETTLGKEIAEKYGMNGLE
CCCHHHCCEEEECCEEECEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCH
VTEDVFESEYSIVFDEAENRMHTIKAVMVATLGS
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFNLRNRNFLKLLDFTPKEIAHLLELSAELKKAKYSGYEQPRLTGKNIALIFEKSSTRT
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
RCAFEVAAFDQGAKVTYLGPSGSQIGHKESMKDTARVLGRMYDGIEYRGFGQTIVEELGQ
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADFLTMQEYANGKQLSQITFAYLGDARNNMGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCEEEEEHHH
MGMEIRLVAPKQFWPEEELVTQCQEIALQTGAKIVLTEEVTEGVKGCDFLYTDVWVSMGE
CCEEEEEECCHHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCC
APEAWDERVALMTPYQINMDVIKATENPNVKFMHCLPAFHNDETTLGKEIAEKYGMNGLE
CCCHHHCCEEEECCEEECEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCH
VTEDVFESEYSIVFDEAENRMHTIKAVMVATLGS
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA