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The map label for this gene is ptsN [H]

Identifier: 59710992

GI number: 59710992

Start: 420158

End: 420601

Strand: Reverse

Name: ptsN [H]

Synonym: VF_0385

Alternate gene names: 59710992

Gene position: 420601-420158 (Counterclockwise)

Preceding gene: 59710993

Following gene: 59710991

Centisome position: 14.52

GC content: 39.41

Gene sequence:

>444_bases
ATGCAGTTAAGCACCATTCTTTCATTGGATTGCACGAAAAGTGCAGTCCAATGCACAAGCAAAAAGCGCGCATTAGAACT
TATTAGTGAAATTGCCGCACCTCGAATTGGTCAAAGCCCTCAAGAACTTTTTGAAAGCTTATTAAACCGAGAAAAAATGG
GAAGTACTGGTATAGGTAATGGGATAGCTATCCCCCATGGCCGAATGTTCAATTGTGATAATGCCGTAGCTGTACTTATC
CAATGCCAAGAACCCATCGAATTTGATGCTATTGATAATCGTCCTGTTGATATTCTTTTTGCACTTCTCGTTCCCGATGA
ACAATGTAAGCTTCATCTAAAAACACTTTCTAGTGTTGCTGAAAAACTCAATGATAAACAAGTATTACGTCAGCTGCGCC
ATGCGGAAAATGATGACGAGCTTTATCAAATCATCACATCTTAA

Upstream 100 bases:

>100_bases
CATTCTGACTCAGAAGATATGTATGCAGCTATTGATGGTTTGGTTGATAAATTGATCCGCCAACTCAATAAACATAAAGA
AAAACTAAGTAGTCATTAAC

Downstream 100 bases:

>100_bases
CCATCTTTTTTAAGGTCATAATAATGAAACTCATGGTAGTTAGCGGAAGCTCTGGTGCAGGTAAATCTGTCGCTTTACGT
GTATTAGAAGATCTTGGTTA

Product: sugar-specific enzyme IIA component of PTS

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPRIGQSPQELFESLLNREKMGSTGIGNGIAIPHGRMFNCDNAVAVLI
QCQEPIEFDAIDNRPVDILFALLVPDEQCKLHLKTLSSVAEKLNDKQVLRQLRHAENDDELYQIITS

Sequences:

>Translated_147_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPRIGQSPQELFESLLNREKMGSTGIGNGIAIPHGRMFNCDNAVAVLI
QCQEPIEFDAIDNRPVDILFALLVPDEQCKLHLKTLSSVAEKLNDKQVLRQLRHAENDDELYQIITS
>Mature_147_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPRIGQSPQELFESLLNREKMGSTGIGNGIAIPHGRMFNCDNAVAVLI
QCQEPIEFDAIDNRPVDILFALLVPDEQCKLHLKTLSSVAEKLNDKQVLRQLRHAENDDELYQIITS

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=147, Percent_Identity=61.2244897959184, Blast_Score=185, Evalue=1e-48,
Organism=Escherichia coli, GI1786951, Length=144, Percent_Identity=26.3888888888889, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16332; Mature: 16332

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPRIGQSPQELFESLLNREKMGSTGIGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRMFNCDNAVAVLIQCQEPIEFDAIDNRPVDILFALLVPDEQCKLHLKTLSSVA
CEECCCCCEECCCHHEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EKLNDKQVLRQLRHAENDDELYQIITS
HHCCHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPRIGQSPQELFESLLNREKMGSTGIGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRMFNCDNAVAVLIQCQEPIEFDAIDNRPVDILFALLVPDEQCKLHLKTLSSVA
CEECCCCCEECCCHHEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EKLNDKQVLRQLRHAENDDELYQIITS
HHCCHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7876255; 8444818; 8025669; 9278503; 9636714 [H]