Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 59710988

GI number: 59710988

Start: 416027

End: 417370

Strand: Direct

Name: pmbA [H]

Synonym: VF_0381

Alternate gene names: 59710988

Gene position: 416027-417370 (Clockwise)

Preceding gene: 59710986

Following gene: 59711001

Centisome position: 14.36

GC content: 41.0

Gene sequence:

>1344_bases
ATGGGCGCAAAACAGCAAATGATGAATCAAAAAATTGAACTCGAAGCAGCGGTAGCTAAAGCTTTAGACATGGCGACACA
GCATGCAAGTGCAGCAGAAGTTGCTATTACAAAAACGACGGGCATTAGTGTATCAACACTTATGGGTGAGGTTGAGAATG
TTGAATTTAACCGCGATGGTGCGTTAGGCATCACTGTATACCGTGGTCATCGTAAAGGTAGCGCATCAACATCTGATTTA
AGTGATAAAGCGATTCAACAAACGGTTGCTGCAGCACTGGATATTGCAAACTACACATCTGAAGACCCGTTTTCAGGTCC
AGCACCAAAAGAATTAATGGCGAAAGATATTCCTGATTTGGATTTATTTCACCCAGATGAGCCAGAGCCAGATTATGCCG
CACAAAAAGCCATTGAAGCTGAGCAAGCGGCTTTAGAGTATCACTCATCAATTAAGCAAAGCGACGGTGCTAGCTACGAT
AGTCATTATGGCATTAAAGTTTATGGTAACAGCCATGGTATGTTGGCAAGTTACCCTTCAAGTCGTCAAAGCATCAGTTG
TAGTGTGATTGGTGTTGATAAAAATGGCGATATGGAGCGTGACTATAGCTACACCGTTGCTCGTCATCGTGATGATTTGT
GGGATGCTAAAAAAGTAGGTTTAGAAGCCGCAAAACGCACAGTAAGTCGTTTAGATGCGAAAAAACTAGATACTATGAAA
GTACCTGTAATGTTTGCTGCTGACGTTGCTACAGGTTTATTAGGGCACTTAGTTATGGCGATCAGTGGTGCTAACTTATA
CCGTAAATCGACATTTATGCTTGATAAGCTAAATGAGAAGATTTTACCTGAATGGTTTAACGTAAGTGAGCGTCCACATA
TTTTAAAAGGACTGGCAAGTAGTCCATTTGATAGTGAAGGCTTATATACTCAAGACCGTGAAATCATTACCGATGGTGTA
TTAGCTACATATCTACTAACTAGCTATGCTGCACGTAAATTAAACATGACACCAACAGGCCATGCTGGTGGTATCCATAA
CTGGTTTGTTAAATCAACAGGTCAAAGCTTTGAGCAAATGCTGAAAACCATGGGTACAGGTCTTTTAGTTACAGAGCTTA
TGGGACAAGGTGTGAATACGGTAACGGGTGATTACTCTCGTGGAGCAGCAGGTTTTTGGGTTGAGAATGGTGAAATTCAA
TATCCGGTCTCTGAAATTACCATTGCAGGTAATCTTGCTGACATGTATCACAATATTGTTGCAGTAGGTAATGATGTAGA
AACTCGCTCTCAAATTCAAACGGGTTCTATTTTAATTGAATCAATGAAGATTGCTGGTCAGTAG

Upstream 100 bases:

>100_bases
GGTGATTGACTCTATTTTAGCAAGATAAATGAATAATTGGGCGCAAATTTATCACATAGAATGTTATTCTAGTAAAAATG
TATCTATTTAGTCAGATGTT

Downstream 100 bases:

>100_bases
TCGTAATATGATCAAATACTTATGTAGATTGGTGTAAAGCATAAATAAAAAAGCGCGCAATCATTAAATGAGAGCGCGCT
TTTTTTATGTTATGTAAAAA

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGAKQQMMNQKIELEAAVAKALDMATQHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDL
SDKAIQQTVAAALDIANYTSEDPFSGPAPKELMAKDIPDLDLFHPDEPEPDYAAQKAIEAEQAALEYHSSIKQSDGASYD
SHYGIKVYGNSHGMLASYPSSRQSISCSVIGVDKNGDMERDYSYTVARHRDDLWDAKKVGLEAAKRTVSRLDAKKLDTMK
VPVMFAADVATGLLGHLVMAISGANLYRKSTFMLDKLNEKILPEWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGV
LATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFEQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQ
YPVSEITIAGNLADMYHNIVAVGNDVETRSQIQTGSILIESMKIAGQ

Sequences:

>Translated_447_residues
MGAKQQMMNQKIELEAAVAKALDMATQHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDL
SDKAIQQTVAAALDIANYTSEDPFSGPAPKELMAKDIPDLDLFHPDEPEPDYAAQKAIEAEQAALEYHSSIKQSDGASYD
SHYGIKVYGNSHGMLASYPSSRQSISCSVIGVDKNGDMERDYSYTVARHRDDLWDAKKVGLEAAKRTVSRLDAKKLDTMK
VPVMFAADVATGLLGHLVMAISGANLYRKSTFMLDKLNEKILPEWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGV
LATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFEQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQ
YPVSEITIAGNLADMYHNIVAVGNDVETRSQIQTGSILIESMKIAGQ
>Mature_446_residues
GAKQQMMNQKIELEAAVAKALDMATQHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDLS
DKAIQQTVAAALDIANYTSEDPFSGPAPKELMAKDIPDLDLFHPDEPEPDYAAQKAIEAEQAALEYHSSIKQSDGASYDS
HYGIKVYGNSHGMLASYPSSRQSISCSVIGVDKNGDMERDYSYTVARHRDDLWDAKKVGLEAAKRTVSRLDAKKLDTMKV
PVMFAADVATGLLGHLVMAISGANLYRKSTFMLDKLNEKILPEWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGVL
ATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFEQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQY
PVSEITIAGNLADMYHNIVAVGNDVETRSQIQTGSILIESMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=64.2058165548098, Blast_Score=592, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48465; Mature: 48334

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAKQQMMNQKIELEAAVAKALDMATQHASAAEVAITKTTGISVSTLMGEVENVEFNRDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCEECCCC
ALGITVYRGHRKGSASTSDLSDKAIQQTVAAALDIANYTSEDPFSGPAPKELMAKDIPDL
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCC
DLFHPDEPEPDYAAQKAIEAEQAALEYHSSIKQSDGASYDSHYGIKVYGNSHGMLASYPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCC
SRQSISCSVIGVDKNGDMERDYSYTVARHRDDLWDAKKVGLEAAKRTVSRLDAKKLDTMK
CCCEEEEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VPVMFAADVATGLLGHLVMAISGANLYRKSTFMLDKLNEKILPEWFNVSERPHILKGLAS
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCC
SPFDSEGLYTQDREIITDGVLATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFEQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
LKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLADMYHNIV
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHE
AVGNDVETRSQIQTGSILIESMKIAGQ
EECCCCHHHHHHHHHHHHEEHHHHCCC
>Mature Secondary Structure 
GAKQQMMNQKIELEAAVAKALDMATQHASAAEVAITKTTGISVSTLMGEVENVEFNRDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCEECCCC
ALGITVYRGHRKGSASTSDLSDKAIQQTVAAALDIANYTSEDPFSGPAPKELMAKDIPDL
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCC
DLFHPDEPEPDYAAQKAIEAEQAALEYHSSIKQSDGASYDSHYGIKVYGNSHGMLASYPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCC
SRQSISCSVIGVDKNGDMERDYSYTVARHRDDLWDAKKVGLEAAKRTVSRLDAKKLDTMK
CCCEEEEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VPVMFAADVATGLLGHLVMAISGANLYRKSTFMLDKLNEKILPEWFNVSERPHILKGLAS
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCC
SPFDSEGLYTQDREIITDGVLATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFEQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
LKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLADMYHNIV
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHE
AVGNDVETRSQIQTGSILIESMKIAGQ
EECCCCHHHHHHHHHHHHEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]