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The map label for this gene is yafV [C]

Identifier: 59710985

GI number: 59710985

Start: 412928

End: 413746

Strand: Direct

Name: yafV [C]

Synonym: VF_0378

Alternate gene names: 59710985

Gene position: 412928-413746 (Clockwise)

Preceding gene: 59710984

Following gene: 59710986

Centisome position: 14.25

GC content: 40.78

Gene sequence:

>819_bases
ATGAGTAAAGTTGGTATTGTTCAGATGACTTCGGGGGCGGAGCCTGATGAGAATATTAAGCAGCTAAAACTCAAACTAAA
AGGGCTACAACTACAAGGTGCTAAGTTAGTTTTAACGCCAGAAAATTGCATAGTATTTGGGCAAAAAGACGATTATGAGC
GATATGCCGAGCCAGTAGGTAAAGGTGTATTACAAGATCAATTGTCAGCGCTTGCTCGTCACTATCAATTGTGGCTTGTG
ATAGGTTCATTTCCTACAAGAAATGAAAATGGCAGTTTATCGACAACCAGCCTTGTATTTGATGATAATGGTCATTTAGT
CGAACATTACAATAAATTGCATATGTTTGATGTTGATGTCGAAGATAGACATCAGAGTTATCGGGAGTCCGATACTTTTA
CGGCTGGTAATGACATTAAAATCGTAGATACTCCTATTGGGAAAGTAGGGCTATCTATTTGCTATGATGTGCGTTTTCCT
CAGTTGTACAGTGAATTACGTAAACAGGGTGCTGAAATTATTCTTGTTCCCGCGGCTTTTACTAAAGTTACCGGCTATGC
ACATTGGGACATATTATTACGCTCAAGAGCCATAGAAACTCAGTGTTGGGTACTTGCTGCAGGGCAGTGGGGTAAACATG
GCGCTGGACGTGAAACATGGGGGCATTCAATGATTATTGACCCGTGGGGTAATAAAGTGACCGCACAACAAGAAGGCACT
GGTGTTATTATTGCTGATATAGACCTTGAGCAAATGAATCAAATCCGCAAAAAAATGCCCGTAGCGCAACACGCTCGTTT
AATTAGTTATCTACCTTAA

Upstream 100 bases:

>100_bases
TTGAAGGACCGCTAGATTCACCTACAGTTAAAGAGCTATCTCGTAGCCAAGGTGAATATCAATTACCTGAAAAAACGGAA
AAATAAGAAAAGGACGTATT

Downstream 100 bases:

>100_bases
ATACAGAAAAGAGTGAGTTTAGCCTATAAAACTCACTCTTTTTTCTTCATAATTAAACGATAGATTTTCAATTCTTAAAA
AAGAGTATTCCTAATGAGTT

Product: beta-ureidopropionase

Products: beta-alanine; CO2; NH3

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSKVGIVQMTSGAEPDENIKQLKLKLKGLQLQGAKLVLTPENCIVFGQKDDYERYAEPVGKGVLQDQLSALARHYQLWLV
IGSFPTRNENGSLSTTSLVFDDNGHLVEHYNKLHMFDVDVEDRHQSYRESDTFTAGNDIKIVDTPIGKVGLSICYDVRFP
QLYSELRKQGAEIILVPAAFTKVTGYAHWDILLRSRAIETQCWVLAAGQWGKHGAGRETWGHSMIIDPWGNKVTAQQEGT
GVIIADIDLEQMNQIRKKMPVAQHARLISYLP

Sequences:

>Translated_272_residues
MSKVGIVQMTSGAEPDENIKQLKLKLKGLQLQGAKLVLTPENCIVFGQKDDYERYAEPVGKGVLQDQLSALARHYQLWLV
IGSFPTRNENGSLSTTSLVFDDNGHLVEHYNKLHMFDVDVEDRHQSYRESDTFTAGNDIKIVDTPIGKVGLSICYDVRFP
QLYSELRKQGAEIILVPAAFTKVTGYAHWDILLRSRAIETQCWVLAAGQWGKHGAGRETWGHSMIIDPWGNKVTAQQEGT
GVIIADIDLEQMNQIRKKMPVAQHARLISYLP
>Mature_271_residues
SKVGIVQMTSGAEPDENIKQLKLKLKGLQLQGAKLVLTPENCIVFGQKDDYERYAEPVGKGVLQDQLSALARHYQLWLVI
GSFPTRNENGSLSTTSLVFDDNGHLVEHYNKLHMFDVDVEDRHQSYRESDTFTAGNDIKIVDTPIGKVGLSICYDVRFPQ
LYSELRKQGAEIILVPAAFTKVTGYAHWDILLRSRAIETQCWVLAAGQWGKHGAGRETWGHSMIIDPWGNKVTAQQEGTG
VIIADIDLEQMNQIRKKMPVAQHARLISYLP

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=269, Percent_Identity=38.2899628252788, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=38.2899628252788, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI297632348, Length=269, Percent_Identity=38.2899628252788, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=33.0827067669173, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=32.6424870466321, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786412, Length=251, Percent_Identity=27.4900398406374, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=267, Percent_Identity=37.4531835205993, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=34.6666666666667, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323383, Length=276, Percent_Identity=32.6086956521739, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17933642, Length=267, Percent_Identity=36.7041198501873, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21355835, Length=271, Percent_Identity=33.210332103321, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.1.6

Molecular weight: Translated: 30517; Mature: 30386

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVGIVQMTSGAEPDENIKQLKLKLKGLQLQGAKLVLTPENCIVFGQKDDYERYAEPVG
CCCCEEEEEECCCCCCHHHHHHHHHHCCEEEECCEEEEECCCEEEECCCCCHHHHHHHHC
KGVLQDQLSALARHYQLWLVIGSFPTRNENGSLSTTSLVFDDNGHLVEHYNKLHMFDVDV
CHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEECCEEEEEECCH
EDRHQSYRESDTFTAGNDIKIVDTPIGKVGLSICYDVRFPQLYSELRKQGAEIILVPAAF
HHHHHHHHCCCCEECCCCEEEEECCCCHHCEEEEEECCCHHHHHHHHHCCCCEEEEECHH
TKVTGYAHWDILLRSRAIETQCWVLAAGQWGKHGAGRETWGHSMIIDPWGNKVTAQQEGT
HHHCCCEEEEEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCC
GVIIADIDLEQMNQIRKKMPVAQHARLISYLP
EEEEEECCHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
SKVGIVQMTSGAEPDENIKQLKLKLKGLQLQGAKLVLTPENCIVFGQKDDYERYAEPVG
CCCEEEEEECCCCCCHHHHHHHHHHCCEEEECCEEEEECCCEEEECCCCCHHHHHHHHC
KGVLQDQLSALARHYQLWLVIGSFPTRNENGSLSTTSLVFDDNGHLVEHYNKLHMFDVDV
CHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEECCEEEEEECCH
EDRHQSYRESDTFTAGNDIKIVDTPIGKVGLSICYDVRFPQLYSELRKQGAEIILVPAAF
HHHHHHHHCCCCEECCCCEEEEECCCCHHCEEEEEECCCHHHHHHHHHCCCCEEEEECHH
TKVTGYAHWDILLRSRAIETQCWVLAAGQWGKHGAGRETWGHSMIIDPWGNKVTAQQEGT
HHHCCCEEEEEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCC
GVIIADIDLEQMNQIRKKMPVAQHARLISYLP
EEEEEECCHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-carbamoyl-beta-alanine; H2O

Specific reaction: N-carbamoyl-beta-alanine + H2O = beta-alanine + CO2 + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]