Click here to switch to the map view.

The map label for this gene is lysC [H]

Identifier: 59710945

GI number: 59710945

Start: 363505

End: 364857

Strand: Reverse

Name: lysC [H]

Synonym: VF_0338

Alternate gene names: 59710945

Gene position: 364857-363505 (Counterclockwise)

Preceding gene: 59710947

Following gene: 59710943

Centisome position: 12.59

GC content: 38.29

Gene sequence:

>1353_bases
GTGAACCCTATTAATGTTGCCAAATTTGGCGGTACTAGCGTTGCAAATTTCGAGGCAATGACGAAAAGTGCAAGTGTCAT
TGAACAAAATCCAAATACTCACCTTGTAGTGATTAGCGCCTGCTCTGGCGTGACTAATCTTCTTGTTGAGCTTGCTAATG
GCGTTCAAGATAATCACAGAAAAAAAGCAATTCTTGAAGGATTAAAAACCATTCATTTTTCTGTGTTAGATCAGCTCGCA
GATCCACTTATTCCAGAGCAAGCTATATTAGATATATTGGATGATATTGAAAAAACAGCAGAAACGGCAGCATCTCAATC
AAATGATAAATTAACCGATCATCTAGTCTCTTGTGGTGAGCTCATGTCTACTCACTTATTCACTCAATTACTCATTGAGC
GAGGAAACCCTGCAGTACGTTTTGATATTCGCACTGTTTTGCGCACTGATTCTCGTTTTGGAAAAGGTGAACCACAACTT
AATGATATATCTCGTTTAGCTCAAAAAAAACTGACTCCTCTTTGTCAACAACAGATTGTGGTGACTCAAGGGTTTATTGG
TTCTGATAGCGAAGGAAATACAACAACTTTGGGTCGTGGTGGCAGTGATTATAGTGCAGCACTGATTGCTGAGTCTGTAG
ATGCATGCGGATTAGAAATCTGGACAGATGTACCAGGGATTTATACCACGGATCCTCGCATTGCTCCCAAAGCGTCACCG
ATTCCAGAAATTAGCTTTAGCGAAGCCTCTGAAATGGCAAATTACGGTGCAAAGATCTTACACCCATCAACACTTCTCCC
TGCGCTTCGTCATCAAATTCCTGTTTTTGTTGGCTCATCAAAAGAACCAGAAAAAGGAGGGACTTGGATTAAAGAAAAAG
TTGATAGTACTCCTTTATTTAGGGCGCTAACCCTTCGTTGTAACCAAACAATGGTAACACTGCATAGCCCAAGTATGTTC
CATGCATATGGCTTCTTAGCTGAAGTTTTCAAGATTTTAGCTAAATACCAAATTTCTGTAGATTTAATTACAACTTCAGA
AGTAAGCGTTGCTCTTACTTTAGATCAAACAAACACCAATGGTAAAGCTCCAGAGCTTCCACTTGAAGCACAACAAGAAT
TAGAAGAATTATGTACTGTTGAAGTTGAACATAATCTTTGTTTAATCGCATTAATAGGTAACAAAATGAGTCATTCAAAA
GGCTCAGCAAAACAAGTGTTTGGTACTTTAGAGGAGTTTAATTTACGTATGATTTGTTATGGTGCTAGTCAACATAACTT
ATGTTTCTTACTTCATGAGTCTGAATCTAAACAAGCCGTTAAAATCTTGCACAAAGAACTTTTTGAACAATAA

Upstream 100 bases:

>100_bases
TCACTTAATTTATTAAGTGAAGAGCTTCTAGCAAACCACTCTTATTGCTATCGCTTCTTCACATTTATTTTTACACTGGA
TGTAGTCGGGAGACAGGATA

Downstream 100 bases:

>100_bases
GTGATACATACAAAAATGCCCATTACATTTAATGGGCATTTTTAGTTTAAAAGGTCAATTCACTATTAATTCAAATTTGG
ACCTAACCACTTCTCAGCTT

Product: aspartate kinase III

Products: NA

Alternate protein names: Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNPINVAKFGGTSVANFEAMTKSASVIEQNPNTHLVVISACSGVTNLLVELANGVQDNHRKKAILEGLKTIHFSVLDQLA
DPLIPEQAILDILDDIEKTAETAASQSNDKLTDHLVSCGELMSTHLFTQLLIERGNPAVRFDIRTVLRTDSRFGKGEPQL
NDISRLAQKKLTPLCQQQIVVTQGFIGSDSEGNTTTLGRGGSDYSAALIAESVDACGLEIWTDVPGIYTTDPRIAPKASP
IPEISFSEASEMANYGAKILHPSTLLPALRHQIPVFVGSSKEPEKGGTWIKEKVDSTPLFRALTLRCNQTMVTLHSPSMF
HAYGFLAEVFKILAKYQISVDLITTSEVSVALTLDQTNTNGKAPELPLEAQQELEELCTVEVEHNLCLIALIGNKMSHSK
GSAKQVFGTLEEFNLRMICYGASQHNLCFLLHESESKQAVKILHKELFEQ

Sequences:

>Translated_450_residues
MNPINVAKFGGTSVANFEAMTKSASVIEQNPNTHLVVISACSGVTNLLVELANGVQDNHRKKAILEGLKTIHFSVLDQLA
DPLIPEQAILDILDDIEKTAETAASQSNDKLTDHLVSCGELMSTHLFTQLLIERGNPAVRFDIRTVLRTDSRFGKGEPQL
NDISRLAQKKLTPLCQQQIVVTQGFIGSDSEGNTTTLGRGGSDYSAALIAESVDACGLEIWTDVPGIYTTDPRIAPKASP
IPEISFSEASEMANYGAKILHPSTLLPALRHQIPVFVGSSKEPEKGGTWIKEKVDSTPLFRALTLRCNQTMVTLHSPSMF
HAYGFLAEVFKILAKYQISVDLITTSEVSVALTLDQTNTNGKAPELPLEAQQELEELCTVEVEHNLCLIALIGNKMSHSK
GSAKQVFGTLEEFNLRMICYGASQHNLCFLLHESESKQAVKILHKELFEQ
>Mature_450_residues
MNPINVAKFGGTSVANFEAMTKSASVIEQNPNTHLVVISACSGVTNLLVELANGVQDNHRKKAILEGLKTIHFSVLDQLA
DPLIPEQAILDILDDIEKTAETAASQSNDKLTDHLVSCGELMSTHLFTQLLIERGNPAVRFDIRTVLRTDSRFGKGEPQL
NDISRLAQKKLTPLCQQQIVVTQGFIGSDSEGNTTTLGRGGSDYSAALIAESVDACGLEIWTDVPGIYTTDPRIAPKASP
IPEISFSEASEMANYGAKILHPSTLLPALRHQIPVFVGSSKEPEKGGTWIKEKVDSTPLFRALTLRCNQTMVTLHSPSMF
HAYGFLAEVFKILAKYQISVDLITTSEVSVALTLDQTNTNGKAPELPLEAQQELEELCTVEVEHNLCLIALIGNKMSHSK
GSAKQVFGTLEEFNLRMICYGASQHNLCFLLHESESKQAVKILHKELFEQ

Specific function: Lysine biosynthesis; first step. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1790455, Length=450, Percent_Identity=54.8888888888889, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1786183, Length=464, Percent_Identity=28.8793103448276, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1790376, Length=335, Percent_Identity=29.2537313432836, Blast_Score=128, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320893, Length=492, Percent_Identity=29.4715447154472, Blast_Score=183, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49179; Mature: 49179

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPINVAKFGGTSVANFEAMTKSASVIEQNPNTHLVVISACSGVTNLLVELANGVQDNHR
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHH
KKAILEGLKTIHFSVLDQLADPLIPEQAILDILDDIEKTAETAASQSNDKLTDHLVSCGE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LMSTHLFTQLLIERGNPAVRFDIRTVLRTDSRFGKGEPQLNDISRLAQKKLTPLCQQQIV
HHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHH
VTQGFIGSDSEGNTTTLGRGGSDYSAALIAESVDACGLEIWTDVPGIYTTDPRIAPKASP
HCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEECCCCCCEECCCCCCCCCCC
IPEISFSEASEMANYGAKILHPSTLLPALRHQIPVFVGSSKEPEKGGTWIKEKVDSTPLF
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCHHH
RALTLRCNQTMVTLHSPSMFHAYGFLAEVFKILAKYQISVDLITTSEVSVALTLDQTNTN
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEEEECCCCC
GKAPELPLEAQQELEELCTVEVEHNLCLIALIGNKMSHSKGSAKQVFGTLEEFNLRMICY
CCCCCCCCHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEE
GASQHNLCFLLHESESKQAVKILHKELFEQ
ECCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPINVAKFGGTSVANFEAMTKSASVIEQNPNTHLVVISACSGVTNLLVELANGVQDNHR
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHH
KKAILEGLKTIHFSVLDQLADPLIPEQAILDILDDIEKTAETAASQSNDKLTDHLVSCGE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LMSTHLFTQLLIERGNPAVRFDIRTVLRTDSRFGKGEPQLNDISRLAQKKLTPLCQQQIV
HHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHH
VTQGFIGSDSEGNTTTLGRGGSDYSAALIAESVDACGLEIWTDVPGIYTTDPRIAPKASP
HCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEECCCCCCEECCCCCCCCCCC
IPEISFSEASEMANYGAKILHPSTLLPALRHQIPVFVGSSKEPEKGGTWIKEKVDSTPLF
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCHHH
RALTLRCNQTMVTLHSPSMFHAYGFLAEVFKILAKYQISVDLITTSEVSVALTLDQTNTN
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEEEECCCCC
GKAPELPLEAQQELEELCTVEVEHNLCLIALIGNKMSHSKGSAKQVFGTLEEFNLRMICY
CCCCCCCCHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEE
GASQHNLCFLLHESESKQAVKILHKELFEQ
ECCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3003049; 8265357; 9278503; 6312411 [H]