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The map label for this gene is cysN [H]

Identifier: 59710928

GI number: 59710928

Start: 330459

End: 331895

Strand: Direct

Name: cysN [H]

Synonym: VF_0321

Alternate gene names: 59710928

Gene position: 330459-331895 (Clockwise)

Preceding gene: 59710927

Following gene: 59710929

Centisome position: 11.4

GC content: 39.04

Gene sequence:

>1437_bases
ATGAATAGTGCAGTAGAGCAACAACTGGCAGAGCTTGGTATTGAAGCTTATTTAAAAGAACATCAATACAAATCATTACT
TCGTTTTTTAACGTGTGGATCAGTAGATGATGGTAAAAGTACCCTCATAGGCCGATTACTCCATGATTCAAAACAAATCT
ATGCAGATCAACTAGATGCAGTTCATGCTGACAGCCAACGAGTGGGTACTACGGGTGAGCGTCCAGATTTAGCTTTATTG
GTTGATGGCCTGCAAGCTGAACGTGAGCAAGGTATTACGATTGATGTGGCATATCGTTACTTCTCAACTCAAAAACGTAA
ATTCATCATTGCAGATACGCCAGGGCATGAGCAATACACTCGTAATATGGCTACGGGCGCATCAACCTGTAATGTCGCCG
TGATTTTGATTGATGCTCGTAAAGGGGTGCTTGATCAAACTCGCCGTCACTCTTATATCGCAAATCTTCTTGGTATTCGT
CATTTTGTAGTAGCTGTGAATAAAATGGATTTAGTTGAATATTCTCAATCTCGTTTTGAAGAGATTAAAGAGGAATACTT
AGCTTTTTCTAAGAAATTGAATAATCCAAATTTAGATATTTCTATTTTACCTTTATCTGCATTAGAAGGAGATAACGTAG
TAAATCCAAGTGATGCATTATCTTGGTATCAAGGTGAACCACTATTAGAAGTCCTTGAGAATGTGGATATTGATGCAGAT
AAAGGCAATGGTGAGTTTAGATTCCCTGTGCAATACGTTAATCGTCCAAACTTAGATTTTCGTGGTTTTGCTGGAACGGT
CTCTTCTGGTGGAATTTCTGTTGGTGATGAAATTGTGGCTTTACCATCAGGTAAAAAATCGAAAGTCGCTCGTATTGTTA
CCTTTGATGGTGATTTAACATCTGCTCAAGCAGGCCAAGCGGTAACGCTAACACTTGAGGATGAAATTGATATTAGTCGT
GGTGACTTGTTAGTTAAATCACAGTCGAACTTGCAAGCCACGGATCAGTTTAAAGCTGAGATTGTATGGATGACGGAAAA
AGGATTAGAACCGGGTCGTCAATACGATATTAAAATTGCAGGCAAGAAAACCGTTGGGCAAATTGATGCTATTCACCATC
AAGTGAACATCAACAGTCTAGAAAAGTTTGATACTCAAGAGTTGCCGCTGAATGGTATTGGTTTATGTGATGTATCGTTA
ACGGAAACCGTGAGTTTAGATCGTTATCAAGATTGCGCAGATACTGGCGGGTTTATTTTTATTGATCGTCTAACTAATGT
AACGGTAGGGGCTGGGATGATCCAAAACCTATCTGATTTGAGTGAGAGTAAGCCAATTAATGACAATATCAGTGCGTTTG
AAATTGAGCTAAATGCGTTAATTCGTAAGCACTTCCCGCATTGGGAAACCAAAGATATTTCTAAGCTTTTAGGCTAA

Upstream 100 bases:

>100_bases
GTCAAGGACGAGCGATAGATCATGATTCATCTGGCTCCATGGAGCTCAAGAAGCGTCAAGGTTACTTTTAATATCAGGCA
TCTAAGGAAGGATTAAATAA

Downstream 100 bases:

>100_bases
GGACTGTCACTATGGCATGGGAACAAGGATTCGTTCTGGCGTTATTAACTCTGATTATTGTGAGTTTATTGGCAAGTAAG
TTAAAACCAAGCGTCATATT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNSAVEQQLAELGIEAYLKEHQYKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLDAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCNVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVEYSQSRFEEIKEEYLAFSKKLNNPNLDISILPLSALEGDNVVNPSDALSWYQGEPLLEVLENVDIDAD
KGNGEFRFPVQYVNRPNLDFRGFAGTVSSGGISVGDEIVALPSGKKSKVARIVTFDGDLTSAQAGQAVTLTLEDEIDISR
GDLLVKSQSNLQATDQFKAEIVWMTEKGLEPGRQYDIKIAGKKTVGQIDAIHHQVNINSLEKFDTQELPLNGIGLCDVSL
TETVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSDLSESKPINDNISAFEIELNALIRKHFPHWETKDISKLLG

Sequences:

>Translated_478_residues
MNSAVEQQLAELGIEAYLKEHQYKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLDAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCNVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVEYSQSRFEEIKEEYLAFSKKLNNPNLDISILPLSALEGDNVVNPSDALSWYQGEPLLEVLENVDIDAD
KGNGEFRFPVQYVNRPNLDFRGFAGTVSSGGISVGDEIVALPSGKKSKVARIVTFDGDLTSAQAGQAVTLTLEDEIDISR
GDLLVKSQSNLQATDQFKAEIVWMTEKGLEPGRQYDIKIAGKKTVGQIDAIHHQVNINSLEKFDTQELPLNGIGLCDVSL
TETVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSDLSESKPINDNISAFEIELNALIRKHFPHWETKDISKLLG
>Mature_478_residues
MNSAVEQQLAELGIEAYLKEHQYKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLDAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCNVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVEYSQSRFEEIKEEYLAFSKKLNNPNLDISILPLSALEGDNVVNPSDALSWYQGEPLLEVLENVDIDAD
KGNGEFRFPVQYVNRPNLDFRGFAGTVSSGGISVGDEIVALPSGKKSKVARIVTFDGDLTSAQAGQAVTLTLEDEIDISR
GDLLVKSQSNLQATDQFKAEIVWMTEKGLEPGRQYDIKIAGKKTVGQIDAIHHQVNINSLEKFDTQELPLNGIGLCDVSL
TETVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSDLSESKPINDNISAFEIELNALIRKHFPHWETKDISKLLG

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI194018522, Length=415, Percent_Identity=29.6385542168675, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI194097354, Length=415, Percent_Identity=29.6385542168675, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI194018520, Length=415, Percent_Identity=29.6385542168675, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI223555963, Length=452, Percent_Identity=27.8761061946903, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5729864, Length=452, Percent_Identity=27.8761061946903, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI46094014, Length=415, Percent_Identity=28.9156626506024, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI4503475, Length=349, Percent_Identity=28.9398280802292, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4503471, Length=351, Percent_Identity=29.0598290598291, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI34147630, Length=335, Percent_Identity=29.5522388059701, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1789108, Length=474, Percent_Identity=63.9240506329114, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=330, Percent_Identity=26.969696969697, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790412, Length=330, Percent_Identity=26.969696969697, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367247, Length=301, Percent_Identity=25.9136212624585, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=27.6315789473684, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=27.6315789473684, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=29.5454545454545, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=333, Percent_Identity=28.5285285285285, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=333, Percent_Identity=28.5285285285285, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=449, Percent_Identity=26.5033407572383, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=35.5072463768116, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=365, Percent_Identity=27.9452054794521, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=284, Percent_Identity=25.7042253521127, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=373, Percent_Identity=29.4906166219839, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6325337, Length=347, Percent_Identity=28.2420749279539, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=347, Percent_Identity=28.2420749279539, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=435, Percent_Identity=28.0459770114943, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=243, Percent_Identity=32.0987654320988, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550900, Length=450, Percent_Identity=26.8888888888889, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137380, Length=364, Percent_Identity=28.8461538461538, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24652838, Length=346, Percent_Identity=28.9017341040462, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17137572, Length=346, Percent_Identity=28.9017341040462, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI45553807, Length=346, Percent_Identity=28.6127167630058, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553816, Length=346, Percent_Identity=28.6127167630058, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24651721, Length=346, Percent_Identity=28.6127167630058, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17864154, Length=346, Percent_Identity=28.6127167630058, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281363316, Length=326, Percent_Identity=27.6073619631902, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17864358, Length=326, Percent_Identity=27.6073619631902, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921738, Length=103, Percent_Identity=35.9223300970874, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52851; Mature: 52851

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAVEQQLAELGIEAYLKEHQYKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHADSQRVGTTGERPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCNVAVILIDARKGVLDQTRRHSYIANLLGIRHFVVAVNKMDLVEYSQSRFE
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIKEEYLAFSKKLNNPNLDISILPLSALEGDNVVNPSDALSWYQGEPLLEVLENVDIDAD
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCC
KGNGEFRFPVQYVNRPNLDFRGFAGTVSSGGISVGDEIVALPSGKKSKVARIVTFDGDLT
CCCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
SAQAGQAVTLTLEDEIDISRGDLLVKSQSNLQATDQFKAEIVWMTEKGLEPGRQYDIKIA
CCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEE
GKKTVGQIDAIHHQVNINSLEKFDTQELPLNGIGLCDVSLTETVSLDRYQDCADTGGFIF
CCCCHHHHHHHHEEECCCHHHHCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHCCCCEEE
IDRLTNVTVGAGMIQNLSDLSESKPINDNISAFEIELNALIRKHFPHWETKDISKLLG
EEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MNSAVEQQLAELGIEAYLKEHQYKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHADSQRVGTTGERPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCNVAVILIDARKGVLDQTRRHSYIANLLGIRHFVVAVNKMDLVEYSQSRFE
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIKEEYLAFSKKLNNPNLDISILPLSALEGDNVVNPSDALSWYQGEPLLEVLENVDIDAD
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCC
KGNGEFRFPVQYVNRPNLDFRGFAGTVSSGGISVGDEIVALPSGKKSKVARIVTFDGDLT
CCCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
SAQAGQAVTLTLEDEIDISRGDLLVKSQSNLQATDQFKAEIVWMTEKGLEPGRQYDIKIA
CCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEE
GKKTVGQIDAIHHQVNINSLEKFDTQELPLNGIGLCDVSLTETVSLDRYQDCADTGGFIF
CCCCHHHHHHHHEEECCCHHHHCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHCCCCEEE
IDRLTNVTVGAGMIQNLSDLSESKPINDNISAFEIELNALIRKHFPHWETKDISKLLG
EEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA