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The map label for this gene is cpdB [H]

Identifier: 59710926

GI number: 59710926

Start: 327130

End: 329112

Strand: Reverse

Name: cpdB [H]

Synonym: VF_0319

Alternate gene names: 59710926

Gene position: 329112-327130 (Counterclockwise)

Preceding gene: 59710931

Following gene: 59710925

Centisome position: 11.36

GC content: 39.59

Gene sequence:

>1983_bases
ATGAACCTCTCAGCAAAACCTGTTTCAATTGCAGTATTAGCTGGTCTAGGTCTACTTACCCTTTCAGGTTGTAGCCCAAC
AGTTACCGATACCGATGAAGTTATTAATCTACGAGTGGTAGAAACAACAGACATCCATACTAACCTTATGGATTATGACT
ACTACAAAGATAAACCGACTCAAAAAATTGGTTTAGCTCGTACAGCAACATTGGTAAAAGAAGCACGTGCTGAAGTAACA
AACAGTGTTTTAGTTGATAATGGTGATTTATTGCAAGGTAGTCCAATGGGTGACTACATGGCAGATAAAGGCATTGAAGC
TGGTGAAGTTCACCCTGCATATAAAGCGATGAATCAATTAGATTACGATGCAGGTAATATTGGTAACCACGAATTTAACT
ACGGTTTAGAGTTTTTAGCAGAATCAATTAATGATGCTGATTTCCCATACATCAGTGCGAACGTTTATGATGCAAAAACA
AAAGAGCATTATTTCAAACCATACATCATTAAAACTCATAAACTAAAAGATACTGCAGGTGTAGAGCACGAAATCAAAGT
CGGCTACATCGGATTTGTTCCACCACAAATCATGGTATGGGATAAAAAGAATCTTGAAGGTAAAGTTTTTGCTCGTGACA
TAAAAGAAACAGCAGAAGAATTAGTACCTCAAATGAAAGCAGAAGGTGCTGATGTTATCGTTGCAATTCCTCACTCTGGC
GTATCAACGAATCCATACAAACAAGGTGAAGAAAACTCAACCTACTACCTTTCTGAAGTAGACGGTATTGATGCTATTGC
CTTTGGTCACTCACATGCGGTTTTCCCAGGAAAAGGGTTCGATAACCTGCAAGGTATTGATAACGAAAACGGTACAATTA
ACGGCGTTGCTGCTGTTATGCCTGGCCGCTGGGGTAGCCATGTTGGTGTAATGGATTTAACTCTTGAGCAAAAAGATGGC
AAATGGAGTGTAGTAAAAGGTCAATCAGAAGCTCGCCCTATTTTTGATAAAACAAATCAGAAATCACTGGCTGACGCTGA
TAAAGGCATCGTACAAGCTTTAGAAGCGGATCATAAAGGCACGCGTGATTTCGTTAACCAGCCAATTGGTAAAGCTGATG
ATGTTATGTATAGCTTCTTAGCTTTAGTTCAAGATGATCCTACCGTTCAAATCGTTAATCTTGCACAAAAAGATTACGTT
GAACGCTTCATTCAAGGCGACCCTGATTTAGATGGTACTCCAGTACTTTCTGCGGCAGCGCCATTCAAAGCTGGCGGTCG
TGGAAATGATCCAACAAACTTTACAGAAGTTGAATCAGGCCAATTGACGTTCCGTAATGCGGCTGATTTATACCTATACC
CGAATACCTTAGTTGCAATGAAAGTAACGGGTAAAGAAGTAAAAGAGTGGCTTGAATGCTCTGCTGGTCAATTTAAACAA
ATCGACATCAACAGTACTGCACCTCAACAGTTAATTGATTGGGATGGTTTCCGTACGTATAACTTTGATGTCATTGATGG
TGTTAACTACCAAATTGACATTACTCAACCAGCAAAATATGACGGTGACTGTAATTTAATTAATGAAGGTTCTGAGCGTA
TCATCGGTTTAACATACCAAGGCAAACCAATTGATGCGAAGCAAGACTTCCTGATCGCAACTAATAACTACCGAGCTTAC
AGCAATAAGTTCCCAGGCACGGGGGCTGATTTCATCGCTTTTGATTCTCCAGATGAAAACCGTACCGTTCTTGCTAACTA
CATTTCTCGAGTTAGTAAAGAGGCAGGTCAAGTTCAACCATCAGCTGATAATAACTGGTCTTTCGCACCAATAAAAAATG
GCGAAAAACTGGATATTCGCTTTGAAACATCACCAAGCGAAAAAGCAACTCAGTTTATTAAAGATAAAGGTCAATACCCA
ATGAAACGTGTTGCGACTGATGATGTTGGTTTTGCTGTATATCAACTTAACTTAGCAAAATAA

Upstream 100 bases:

>100_bases
AAGATCAACTGCACACATTGAGAGGCCAGTTACATTTATAACATAGCCAATCTGTTATCTTATTGGCGCTTTTATGATTA
TCACCTTTTGGAGACACACT

Downstream 100 bases:

>100_bases
AATTCATCTCTTTTACAAAACAAATAGCATGGTGATTTCACCATGCTATTTTTTTATCTGTATGCTAAGTTTTCCACTAT
TAAAACATCATCTTCTAATA

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MNLSAKPVSIAVLAGLGLLTLSGCSPTVTDTDEVINLRVVETTDIHTNLMDYDYYKDKPTQKIGLARTATLVKEARAEVT
NSVLVDNGDLLQGSPMGDYMADKGIEAGEVHPAYKAMNQLDYDAGNIGNHEFNYGLEFLAESINDADFPYISANVYDAKT
KEHYFKPYIIKTHKLKDTAGVEHEIKVGYIGFVPPQIMVWDKKNLEGKVFARDIKETAEELVPQMKAEGADVIVAIPHSG
VSTNPYKQGEENSTYYLSEVDGIDAIAFGHSHAVFPGKGFDNLQGIDNENGTINGVAAVMPGRWGSHVGVMDLTLEQKDG
KWSVVKGQSEARPIFDKTNQKSLADADKGIVQALEADHKGTRDFVNQPIGKADDVMYSFLALVQDDPTVQIVNLAQKDYV
ERFIQGDPDLDGTPVLSAAAPFKAGGRGNDPTNFTEVESGQLTFRNAADLYLYPNTLVAMKVTGKEVKEWLECSAGQFKQ
IDINSTAPQQLIDWDGFRTYNFDVIDGVNYQIDITQPAKYDGDCNLINEGSERIIGLTYQGKPIDAKQDFLIATNNYRAY
SNKFPGTGADFIAFDSPDENRTVLANYISRVSKEAGQVQPSADNNWSFAPIKNGEKLDIRFETSPSEKATQFIKDKGQYP
MKRVATDDVGFAVYQLNLAK

Sequences:

>Translated_660_residues
MNLSAKPVSIAVLAGLGLLTLSGCSPTVTDTDEVINLRVVETTDIHTNLMDYDYYKDKPTQKIGLARTATLVKEARAEVT
NSVLVDNGDLLQGSPMGDYMADKGIEAGEVHPAYKAMNQLDYDAGNIGNHEFNYGLEFLAESINDADFPYISANVYDAKT
KEHYFKPYIIKTHKLKDTAGVEHEIKVGYIGFVPPQIMVWDKKNLEGKVFARDIKETAEELVPQMKAEGADVIVAIPHSG
VSTNPYKQGEENSTYYLSEVDGIDAIAFGHSHAVFPGKGFDNLQGIDNENGTINGVAAVMPGRWGSHVGVMDLTLEQKDG
KWSVVKGQSEARPIFDKTNQKSLADADKGIVQALEADHKGTRDFVNQPIGKADDVMYSFLALVQDDPTVQIVNLAQKDYV
ERFIQGDPDLDGTPVLSAAAPFKAGGRGNDPTNFTEVESGQLTFRNAADLYLYPNTLVAMKVTGKEVKEWLECSAGQFKQ
IDINSTAPQQLIDWDGFRTYNFDVIDGVNYQIDITQPAKYDGDCNLINEGSERIIGLTYQGKPIDAKQDFLIATNNYRAY
SNKFPGTGADFIAFDSPDENRTVLANYISRVSKEAGQVQPSADNNWSFAPIKNGEKLDIRFETSPSEKATQFIKDKGQYP
MKRVATDDVGFAVYQLNLAK
>Mature_660_residues
MNLSAKPVSIAVLAGLGLLTLSGCSPTVTDTDEVINLRVVETTDIHTNLMDYDYYKDKPTQKIGLARTATLVKEARAEVT
NSVLVDNGDLLQGSPMGDYMADKGIEAGEVHPAYKAMNQLDYDAGNIGNHEFNYGLEFLAESINDADFPYISANVYDAKT
KEHYFKPYIIKTHKLKDTAGVEHEIKVGYIGFVPPQIMVWDKKNLEGKVFARDIKETAEELVPQMKAEGADVIVAIPHSG
VSTNPYKQGEENSTYYLSEVDGIDAIAFGHSHAVFPGKGFDNLQGIDNENGTINGVAAVMPGRWGSHVGVMDLTLEQKDG
KWSVVKGQSEARPIFDKTNQKSLADADKGIVQALEADHKGTRDFVNQPIGKADDVMYSFLALVQDDPTVQIVNLAQKDYV
ERFIQGDPDLDGTPVLSAAAPFKAGGRGNDPTNFTEVESGQLTFRNAADLYLYPNTLVAMKVTGKEVKEWLECSAGQFKQ
IDINSTAPQQLIDWDGFRTYNFDVIDGVNYQIDITQPAKYDGDCNLINEGSERIIGLTYQGKPIDAKQDFLIATNNYRAY
SNKFPGTGADFIAFDSPDENRTVLANYISRVSKEAGQVQPSADNNWSFAPIKNGEKLDIRFETSPSEKATQFIKDKGQYP
MKRVATDDVGFAVYQLNLAK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=626, Percent_Identity=64.3769968051118, Blast_Score=878, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=600, Percent_Identity=24.5, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72615; Mature: 72615

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSAKPVSIAVLAGLGLLTLSGCSPTVTDTDEVINLRVVETTDIHTNLMDYDYYKDKPT
CCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC
QKIGLARTATLVKEARAEVTNSVLVDNGDLLQGSPMGDYMADKGIEAGEVHPAYKAMNQL
HHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCCCCCCHHHHCCCCCCCCCHHHHHHHHC
DYDAGNIGNHEFNYGLEFLAESINDADFPYISANVYDAKTKEHYFKPYIIKTHKLKDTAG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCEEEEEEECCCCCC
VEHEIKVGYIGFVPPQIMVWDKKNLEGKVFARDIKETAEELVPQMKAEGADVIVAIPHSG
CCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VSTNPYKQGEENSTYYLSEVDGIDAIAFGHSHAVFPGKGFDNLQGIDNENGTINGVAAVM
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCEEEEEEEEC
PGRWGSHVGVMDLTLEQKDGKWSVVKGQSEARPIFDKTNQKSLADADKGIVQALEADHKG
CCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
TRDFVNQPIGKADDVMYSFLALVQDDPTVQIVNLAQKDYVERFIQGDPDLDGTPVLSAAA
CHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
PFKAGGRGNDPTNFTEVESGQLTFRNAADLYLYPNTLVAMKVTGKEVKEWLECSAGQFKQ
CCCCCCCCCCCCCCEEECCCEEEEECCCEEEEECCEEEEEEECCHHHHHHHHCCCCCEEE
IDINSTAPQQLIDWDGFRTYNFDVIDGVNYQIDITQPAKYDGDCNLINEGSERIIGLTYQ
EECCCCCCHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEECCCCCEEEEEEEC
GKPIDAKQDFLIATNNYRAYSNKFPGTGADFIAFDSPDENRTVLANYISRVSKEAGQVQP
CCCCCCCCCEEEEECCCEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
SADNNWSFAPIKNGEKLDIRFETSPSEKATQFIKDKGQYPMKRVATDDVGFAVYQLNLAK
CCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNLSAKPVSIAVLAGLGLLTLSGCSPTVTDTDEVINLRVVETTDIHTNLMDYDYYKDKPT
CCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC
QKIGLARTATLVKEARAEVTNSVLVDNGDLLQGSPMGDYMADKGIEAGEVHPAYKAMNQL
HHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCCCCCCHHHHCCCCCCCCCHHHHHHHHC
DYDAGNIGNHEFNYGLEFLAESINDADFPYISANVYDAKTKEHYFKPYIIKTHKLKDTAG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCEEEEEEECCCCCC
VEHEIKVGYIGFVPPQIMVWDKKNLEGKVFARDIKETAEELVPQMKAEGADVIVAIPHSG
CCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VSTNPYKQGEENSTYYLSEVDGIDAIAFGHSHAVFPGKGFDNLQGIDNENGTINGVAAVM
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCEEEEEEEEC
PGRWGSHVGVMDLTLEQKDGKWSVVKGQSEARPIFDKTNQKSLADADKGIVQALEADHKG
CCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
TRDFVNQPIGKADDVMYSFLALVQDDPTVQIVNLAQKDYVERFIQGDPDLDGTPVLSAAA
CHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
PFKAGGRGNDPTNFTEVESGQLTFRNAADLYLYPNTLVAMKVTGKEVKEWLECSAGQFKQ
CCCCCCCCCCCCCCEEECCCEEEEECCCEEEEECCEEEEEEECCHHHHHHHHCCCCCEEE
IDINSTAPQQLIDWDGFRTYNFDVIDGVNYQIDITQPAKYDGDCNLINEGSERIIGLTYQ
EECCCCCCHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEECCCCCEEEEEEEC
GKPIDAKQDFLIATNNYRAYSNKFPGTGADFIAFDSPDENRTVLANYISRVSKEAGQVQP
CCCCCCCCCEEEEECCCEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
SADNNWSFAPIKNGEKLDIRFETSPSEKATQFIKDKGQYPMKRVATDDVGFAVYQLNLAK
CCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA