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The map label for this gene is thiP [H]

Identifier: 59710875

GI number: 59710875

Start: 271429

End: 273033

Strand: Direct

Name: thiP [H]

Synonym: VF_0268

Alternate gene names: 59710875

Gene position: 271429-273033 (Clockwise)

Preceding gene: 59710874

Following gene: 59710876

Centisome position: 9.37

GC content: 37.2

Gene sequence:

>1605_bases
ATGAAATCTCGAACTTTATTTAATTCCACGCCTTTATTCGGCTATATCGTTGCAGCCATTATTCTCTGCTTCGTTGGTGG
TGCAATTAGTGCGCTTTTAATCGAATCTCCGAGTTTAGATTTACGTATTATTTGGTCTGATCCTTATTACCGCTACATCA
CTCAATTTAGTTTATACCAAGCATTTTTATCAACCATAATCAGTATTGGTTTGGCTATACCAACAGCCTATGCTTTATCT
CGCCGTCAATTTATTGGGCGTGGATTATTACTAAAACTCTGTGCAACGACGTTAGTATTACCAGTGCTTGTGGGGGTTTT
TGGTTTATTAGCTATTTATGGGAACAGTGGATTAATCGCTGATGTTTTTACTTGGTTTGGCGCATCGTTACCATTTTCGA
TTTATGGCCTAAATGGCATTTTATTGGCTCATGTTTTTTTTAATTTCCCTTTTGCAACTCGGCTGTTACTGCAATCATTA
GATTCTATTTCCATTGAACAACATCAGTTATCTGCACACCTTGGATTAATGGGGTGGAGTAAGTATCGTCTTGTTGCATG
GCCGAGAATAAAACAGCAATTGCCACATGTTGCTGGGTTGGTCTTTATGCTTTGCTTTACGAGTTTTGCTACCGTAATGG
CGCTAGGTGGTGGCCCTAAGTCCACAACAATAGAGTTAGCAATATACCAAGCAATTAAGTTTGATTTTGATTTGCAATCA
GGTGCCATTTTAGCTTTATGGCAAATGTTATTATGTGGATTATTATCATTGTGTATTCAACGATTCTCAAAATCAGTTTC
TGTTGATTCTGCATCGTTATCACAAGTGGTTCTATATCAAGATACCAATAAGGCAAAGTGGTGGGACAGAGCGTGGATAA
CGCTTTTAGGGCTTTTTATGTTACCGCCCTTATTTATGGTTGTTCTTAGTGGGCTTAATTCACAATTATTTGTTGTCATC
ACGTCACCGGAGTTTTGGTCTGCATTAATCAATTCTTTAATTGTTGCCTTTGGTGCCGCTGTGATTGCATTACTTCTTGG
TGTTCTGCTTTTACATACAACTCGGTCTTTACGTTTAAAACAAAAAAGATTATTAGCAGATAAGTTAGATCTGATTGGTG
TGATGATTTTAGTCACGCCGGGGTTGGTAATCAGTACGGGGCTTTTTTTATTATTAAGAAACATTACTGATGTATTTAGT
ATTGCTATTTGGATTGTCATCTTAGTTAATGCATTAATGGCATTACCTTATGTAATAAAAACGTTAGCTCAACCTTTATT
GCATATTGCGCAACAGTACCATTTACTGTGTGCAAGTTTGGGATTGTACGGATTGTCACGATTTCGAGTGATTGAATGGA
AAGCATTAAGAAAACCTTTTGCTCATGCTTTTTCCATTGCTTTTATTATGTCTGTTGGTGATTTAAGTGCGATTGCCTTG
TTTGGTAGCCAAGATTTTAGAACGCTACCACTCTATTTATTTCAATTGCTTGGCAGTTATCAGATGGAAGCGGCAGCCGT
TATTTCATTGAGCCTTTTATTATTGAGTGTGGGTGTGTTTGCTGTTGTTGAATGGCTATTTTTATCAAAGAAAAGGAAAC
AGTAA

Upstream 100 bases:

>100_bases
ACATCGCACAAAAGCGTAAATCTTGGATCCGTGAATGGCAACATGCGTTAACTTTTTAATTGGTGTAATAAGGAGCCACT
AGTGCTCCTTTATTTTATTC

Downstream 100 bases:

>100_bases
TGCTGCGTTTTTCTGATGTGAAATATCGATATTATACTGATTTGTTTGAGTTCGATTTGAATGTAGAGCAGGGAAGCGTT
GTTGCTATTTTAGGTCCTAG

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKSRTLFNSTPLFGYIVAAIILCFVGGAISALLIESPSLDLRIIWSDPYYRYITQFSLYQAFLSTIISIGLAIPTAYALS
RRQFIGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLIADVFTWFGASLPFSIYGLNGILLAHVFFNFPFATRLLLQSL
DSISIEQHQLSAHLGLMGWSKYRLVAWPRIKQQLPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQS
GAILALWQMLLCGLLSLCIQRFSKSVSVDSASLSQVVLYQDTNKAKWWDRAWITLLGLFMLPPLFMVVLSGLNSQLFVVI
TSPEFWSALINSLIVAFGAAVIALLLGVLLLHTTRSLRLKQKRLLADKLDLIGVMILVTPGLVISTGLFLLLRNITDVFS
IAIWIVILVNALMALPYVIKTLAQPLLHIAQQYHLLCASLGLYGLSRFRVIEWKALRKPFAHAFSIAFIMSVGDLSAIAL
FGSQDFRTLPLYLFQLLGSYQMEAAAVISLSLLLLSVGVFAVVEWLFLSKKRKQ

Sequences:

>Translated_534_residues
MKSRTLFNSTPLFGYIVAAIILCFVGGAISALLIESPSLDLRIIWSDPYYRYITQFSLYQAFLSTIISIGLAIPTAYALS
RRQFIGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLIADVFTWFGASLPFSIYGLNGILLAHVFFNFPFATRLLLQSL
DSISIEQHQLSAHLGLMGWSKYRLVAWPRIKQQLPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQS
GAILALWQMLLCGLLSLCIQRFSKSVSVDSASLSQVVLYQDTNKAKWWDRAWITLLGLFMLPPLFMVVLSGLNSQLFVVI
TSPEFWSALINSLIVAFGAAVIALLLGVLLLHTTRSLRLKQKRLLADKLDLIGVMILVTPGLVISTGLFLLLRNITDVFS
IAIWIVILVNALMALPYVIKTLAQPLLHIAQQYHLLCASLGLYGLSRFRVIEWKALRKPFAHAFSIAFIMSVGDLSAIAL
FGSQDFRTLPLYLFQLLGSYQMEAAAVISLSLLLLSVGVFAVVEWLFLSKKRKQ
>Mature_534_residues
MKSRTLFNSTPLFGYIVAAIILCFVGGAISALLIESPSLDLRIIWSDPYYRYITQFSLYQAFLSTIISIGLAIPTAYALS
RRQFIGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLIADVFTWFGASLPFSIYGLNGILLAHVFFNFPFATRLLLQSL
DSISIEQHQLSAHLGLMGWSKYRLVAWPRIKQQLPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQS
GAILALWQMLLCGLLSLCIQRFSKSVSVDSASLSQVVLYQDTNKAKWWDRAWITLLGLFMLPPLFMVVLSGLNSQLFVVI
TSPEFWSALINSLIVAFGAAVIALLLGVLLLHTTRSLRLKQKRLLADKLDLIGVMILVTPGLVISTGLFLLLRNITDVFS
IAIWIVILVNALMALPYVIKTLAQPLLHIAQQYHLLCASLGLYGLSRFRVIEWKALRKPFAHAFSIAFIMSVGDLSAIAL
FGSQDFRTLPLYLFQLLGSYQMEAAAVISLSLLLLSVGVFAVVEWLFLSKKRKQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=490, Percent_Identity=47.7551020408163, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59062; Mature: 59062

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRTLFNSTPLFGYIVAAIILCFVGGAISALLIESPSLDLRIIWSDPYYRYITQFSLYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
AFLSTIISIGLAIPTAYALSRRQFIGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DVFTWFGASLPFSIYGLNGILLAHVFFNFPFATRLLLQSLDSISIEQHQLSAHLGLMGWS
HHHHHHCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
KYRLVAWPRIKQQLPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQS
CCEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCC
GAILALWQMLLCGLLSLCIQRFSKSVSVDSASLSQVVLYQDTNKAKWWDRAWITLLGLFM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHH
LPPLFMVVLSGLNSQLFVVITSPEFWSALINSLIVAFGAAVIALLLGVLLLHTTRSLRLK
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKRLLADKLDLIGVMILVTPGLVISTGLFLLLRNITDVFSIAIWIVILVNALMALPYVIK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAQPLLHIAQQYHLLCASLGLYGLSRFRVIEWKALRKPFAHAFSIAFIMSVGDLSAIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
FGSQDFRTLPLYLFQLLGSYQMEAAAVISLSLLLLSVGVFAVVEWLFLSKKRKQ
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSRTLFNSTPLFGYIVAAIILCFVGGAISALLIESPSLDLRIIWSDPYYRYITQFSLYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
AFLSTIISIGLAIPTAYALSRRQFIGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DVFTWFGASLPFSIYGLNGILLAHVFFNFPFATRLLLQSLDSISIEQHQLSAHLGLMGWS
HHHHHHCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
KYRLVAWPRIKQQLPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQS
CCEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCC
GAILALWQMLLCGLLSLCIQRFSKSVSVDSASLSQVVLYQDTNKAKWWDRAWITLLGLFM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHH
LPPLFMVVLSGLNSQLFVVITSPEFWSALINSLIVAFGAAVIALLLGVLLLHTTRSLRLK
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKRLLADKLDLIGVMILVTPGLVISTGLFLLLRNITDVFSIAIWIVILVNALMALPYVIK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAQPLLHIAQQYHLLCASLGLYGLSRFRVIEWKALRKPFAHAFSIAFIMSVGDLSAIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
FGSQDFRTLPLYLFQLLGSYQMEAAAVISLSLLLLSVGVFAVVEWLFLSKKRKQ
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]