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The map label for this gene is gshB [H]

Identifier: 58617414

GI number: 58617414

Start: 1089134

End: 1090072

Strand: Reverse

Name: gshB [H]

Synonym: ERGA_CDS_06870

Alternate gene names: 58617414

Gene position: 1090072-1089134 (Counterclockwise)

Preceding gene: 58617415

Following gene: 58617412

Centisome position: 72.68

GC content: 29.18

Gene sequence:

>939_bases
ATGGCGTTAGTTGTTGCTTTTCAGATGGATAGGGATGTAGTTGTTGGTAAAGATGTATCAGTAAAATTCATACAAGAAGC
GCAAATAAGAGGACATACTGTATTTTTTTTTACTCCTGATAAATTGGCAATAAAAAACAATATTCCAGTTGCTAGTGCTT
GTTTAGTTACTGTAGAACAGGAAAAACTGAAGTTTGAGAATGTACAAGAGTTATTTCTTGAAGATATGGATATAATATTT
ATTCGTCAAAATCCACCTTTTGATATGCGCTATATTACTTCGACTTATATTTTAGAAAAACTGACAAAGCCACTTTTAAT
AAATGATCCTAAATTTCTAAGGGATTATCCTGAAAAATTGTTACCACTTTTATTCGATGATTTTATTCCAGATACTCTAA
TTACTGAAGATTATTCTATGTTGCAAAATTTTTATTATGAACATAAGGATATTATATTAAAACCTCTATATAGTTATGGT
GGAAATGACGTAATTAGGATTAATAATAATGTTGATATTAAGGTAATAGTTGCACTGATGATAGAAAAGTATAAATGTCC
TATTATTGCTCAACAATTTATACCTAATATTAATAATGATAAAAGAATTTTACTATTAGATGGTAAACCTATAGGAGTCT
TTAGGCGTAGAATATTATCTGCTGGAGAGGTAAGAACTAATTTGCGAGTTGGGTCAGTTGCAGAACCTGCTGAATTGTCA
GATCGAGATTATAGTATATGTTTAGCAATAGAGGATAAGTTAGCTTTGAAAGGCTTGATATTTGCTGGAATAGATGTGTT
AGATGGATATTTAATTGAAATTAATGTTACGTCTCCTTGTGGAGTTTTAGAGGTTAATAAGTTATATAATGTATGTTTAG
AAAAAAAATGTTGGGATTGTTTTGAAAAAAAGTTTTATGCAAGATGTGGTTATGTATAG

Upstream 100 bases:

>100_bases
TTCTTGCTTAGTAAATTATCTTGCATAATCCCTATGAAGCTGTGTTAGTATTATATAAAGAAAGAAGATTAAGTTAAATA
AATTTACAAGAGAAATAAGG

Downstream 100 bases:

>100_bases
ATCATTTATTTGTTAAACTAAAGTTATATATATGTACATGTTTTTTATATTATAATTTAATTCTTTCAATATTAACAACA
TTACTATCTTGAGTATGTTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MALVVAFQMDRDVVVGKDVSVKFIQEAQIRGHTVFFFTPDKLAIKNNIPVASACLVTVEQEKLKFENVQELFLEDMDIIF
IRQNPPFDMRYITSTYILEKLTKPLLINDPKFLRDYPEKLLPLLFDDFIPDTLITEDYSMLQNFYYEHKDIILKPLYSYG
GNDVIRINNNVDIKVIVALMIEKYKCPIIAQQFIPNINNDKRILLLDGKPIGVFRRRILSAGEVRTNLRVGSVAEPAELS
DRDYSICLAIEDKLALKGLIFAGIDVLDGYLIEINVTSPCGVLEVNKLYNVCLEKKCWDCFEKKFYARCGYV

Sequences:

>Translated_312_residues
MALVVAFQMDRDVVVGKDVSVKFIQEAQIRGHTVFFFTPDKLAIKNNIPVASACLVTVEQEKLKFENVQELFLEDMDIIF
IRQNPPFDMRYITSTYILEKLTKPLLINDPKFLRDYPEKLLPLLFDDFIPDTLITEDYSMLQNFYYEHKDIILKPLYSYG
GNDVIRINNNVDIKVIVALMIEKYKCPIIAQQFIPNINNDKRILLLDGKPIGVFRRRILSAGEVRTNLRVGSVAEPAELS
DRDYSICLAIEDKLALKGLIFAGIDVLDGYLIEINVTSPCGVLEVNKLYNVCLEKKCWDCFEKKFYARCGYV
>Mature_311_residues
ALVVAFQMDRDVVVGKDVSVKFIQEAQIRGHTVFFFTPDKLAIKNNIPVASACLVTVEQEKLKFENVQELFLEDMDIIFI
RQNPPFDMRYITSTYILEKLTKPLLINDPKFLRDYPEKLLPLLFDDFIPDTLITEDYSMLQNFYYEHKDIILKPLYSYGG
NDVIRINNNVDIKVIVALMIEKYKCPIIAQQFIPNINNDKRILLLDGKPIGVFRRRILSAGEVRTNLRVGSVAEPAELSD
RDYSICLAIEDKLALKGLIFAGIDVLDGYLIEINVTSPCGVLEVNKLYNVCLEKKCWDCFEKKFYARCGYV

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=301, Percent_Identity=35.8803986710963, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35902; Mature: 35770

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVAFQMDRDVVVGKDVSVKFIQEAQIRGHTVFFFTPDKLAIKNNIPVASACLVTVEQ
CEEEEEEECCCCEEECCCCEEEEEEHHCCCCCEEEEECCCCEEEECCCCEEHEEEEEEEH
EKLKFENVQELFLEDMDIIFIRQNPPFDMRYITSTYILEKLTKPLLINDPKFLRDYPEKL
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHH
LPLLFDDFIPDTLITEDYSMLQNFYYEHKDIILKPLYSYGGNDVIRINNNVDIKVIVALM
HHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHEEEHHHCCCCCEEEECCCCCHHHHHHHH
IEKYKCPIIAQQFIPNINNDKRILLLDGKPIGVFRRRILSAGEVRTNLRVGSVAEPAELS
HHHCCCCEEHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEECEEECCCCCCCCCC
DRDYSICLAIEDKLALKGLIFAGIDVLDGYLIEINVTSPCGVLEVNKLYNVCLEKKCWDC
CCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
FEKKFYARCGYV
HHHHHHHHCCCC
>Mature Secondary Structure 
ALVVAFQMDRDVVVGKDVSVKFIQEAQIRGHTVFFFTPDKLAIKNNIPVASACLVTVEQ
EEEEEEECCCCEEECCCCEEEEEEHHCCCCCEEEEECCCCEEEECCCCEEHEEEEEEEH
EKLKFENVQELFLEDMDIIFIRQNPPFDMRYITSTYILEKLTKPLLINDPKFLRDYPEKL
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHH
LPLLFDDFIPDTLITEDYSMLQNFYYEHKDIILKPLYSYGGNDVIRINNNVDIKVIVALM
HHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHEEEHHHCCCCCEEEECCCCCHHHHHHHH
IEKYKCPIIAQQFIPNINNDKRILLLDGKPIGVFRRRILSAGEVRTNLRVGSVAEPAELS
HHHCCCCEEHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEECEEECCCCCCCCCC
DRDYSICLAIEDKLALKGLIFAGIDVLDGYLIEINVTSPCGVLEVNKLYNVCLEKKCWDC
CCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
FEKKFYARCGYV
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1540152 [H]