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The map label for this gene is rne [H]

Identifier: 58617295

GI number: 58617295

Start: 923692

End: 925524

Strand: Reverse

Name: rne [H]

Synonym: ERGA_CDS_05680

Alternate gene names: 58617295

Gene position: 925524-923692 (Counterclockwise)

Preceding gene: 58617296

Following gene: 58617294

Centisome position: 61.7

GC content: 29.3

Gene sequence:

>1833_bases
TTGGTGTCAAATTATAATAAAAGGATATTAATTGATGGGGTATATCAGGATGAAATTAGAGTAGCAGTATTACGTGATGG
TCAGGTTGTAGAATTTGACCAAGAATCAAAAAGCAAAAAGTGTTTGAAGGGTAATATATATTGTGCTGTAGTGAAACGTA
TAGAACCATCTTTACAGGCAGTATTTATTGAATATGGAATAAATAAGCATGGGTTTTTGCCTTTTTCTGAGATATCTTTG
GAATATTATAATATTCCTGAAGAAGAGAAAGAAACATTATATCAAACCTTGTATAAAGATTCTGATAAAGATACTGATAC
AAAAAGTGATGATGAAAAATCTACAGATACAATGATTTTATCTGAAGATGAGAGTGAAGGTATTGCTGAATCTGAAGAAA
AACTAGCATTATTAAAGAAAGAAAACTCAAGAAATAATTATAATAAATCTAAGTTGCCTTTTCATAAGCTATATAAGGTG
CAGGATGTCATAAGGTTGGACCAAAAAATAATGGTTCAAATTATAAAAGAAGAGCGCGGGAATAAAGGTGCGACTTTCTC
AACATTTATTACTTTAGTTGGGAGATATTGCATATTTATGCCTAACTCTGGTAATAAAAATAGTGGGATATCGCGTCGTA
TAGATGATCCTGAAGAAAGAAAAATATTAAAAAATTTTTTAAATTCTATCAAATTACCTAAAAAATCTGGTTTAATTATT
AGGACGGCTGGATCTAATAAAGCTCGAAAAGAAATCGAGCAAGATTTGTCATACTTACAATCAATTTGGCGAAGTATACA
AAATAGTTATATAAGTGTTACTGTACCTTCATTATTGTATATTGAGTGGGATATAATAAAAAGAACATTACGTGATTATT
GTGATGAAAGTACTCAAGATATTGTTGTATCAGGTAGTCATGCATATGAATTAGCTAAAGAATGTATTAAGTTTATGTTT
AGGAATAGGGTACGTCTTAGGATTTATAAAGGTAGTGTACCTATTTTTTCTTATTATAAGATTGAATCTCAAATTTGTGA
ACTATATAGCAATATAGTAAATCTACCTTCTGGTGGATATATGGTTATTACTCCTACGGAAGCTTTGGTATCAATAGATG
TTAATTCCGGTAAAATGACAGGAGAAGATAGTATTGAAGAAACAGCTTATAAAACAAATATGGAGGCTGTTAAAGAAATT
GCAAGACAAGTTAATTTACGTGGTTTATCTGGATTAATAGTAATTGATTTTATTGATATGTTAAAATATCGATATTGTAG
ATCAGTAGAATCTGAAATCAAAGAAGCATTTAAATATGATAAGGCACAAGTTCAGTTTGGGTATATAAGTACATTTGGAT
TGATGGAGATATCAAGGCAAAGGGTAAAGCAAAGTATATTAGAAGCAAATACAGTACCATGTTCACATTGTAAAGGAGTA
GGTAAGGTACGTTCTTTTGAATCATCTTCTGGAGATATACTAAGAAATATTAGATATTGTGCTAATAAAAATCGTAACAA
GTTAATAAATGTTTCAGTAGTTGCGCAAATGGTGGAATATTTTTTTAATAATAAGCGTCAGCATATTACTGAAATTGAAG
CAGAGTTTAGCGTATCAATAAAGTTAACAGTCAGTCAAAATCTTAATAGTATATCTGATTTTAGTATTAGTGTTGAAAAT
GATAATATAGTAAATCAAAGTGAAGATCTGGTAAATGTATTTACTGTTGACTATAAAACTTCTAAGAGTGAAGACTTAAA
AGAGGAAGTTCAAAATTCTACTAGTAGTGTATGGATAAAACGATGGTTAAATCGTATATTTTCGCATATTTAA

Upstream 100 bases:

>100_bases
ATTGAAATGATAAGAAAATAATAAAGTGTTTTTATCTTTAAAATCTTATCTATACTGTTATAATTAGCTACTATAGTTTC
AGTAAGTTTTAGGTGTAAAT

Downstream 100 bases:

>100_bases
TATAAATTGTGTGAACTTATATTAAATGGGGTTGTAATCTTGGTAATTGGTTATTATATCTGTGAATATCGATTTTGATT
TTATGTAGGGGGCTGTTTAT

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MVSNYNKRILIDGVYQDEIRVAVLRDGQVVEFDQESKSKKCLKGNIYCAVVKRIEPSLQAVFIEYGINKHGFLPFSEISL
EYYNIPEEEKETLYQTLYKDSDKDTDTKSDDEKSTDTMILSEDESEGIAESEEKLALLKKENSRNNYNKSKLPFHKLYKV
QDVIRLDQKIMVQIIKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIDDPEERKILKNFLNSIKLPKKSGLII
RTAGSNKARKEIEQDLSYLQSIWRSIQNSYISVTVPSLLYIEWDIIKRTLRDYCDESTQDIVVSGSHAYELAKECIKFMF
RNRVRLRIYKGSVPIFSYYKIESQICELYSNIVNLPSGGYMVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEI
ARQVNLRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAQVQFGYISTFGLMEISRQRVKQSILEANTVPCSHCKGV
GKVRSFESSSGDILRNIRYCANKNRNKLINVSVVAQMVEYFFNNKRQHITEIEAEFSVSIKLTVSQNLNSISDFSISVEN
DNIVNQSEDLVNVFTVDYKTSKSEDLKEEVQNSTSSVWIKRWLNRIFSHI

Sequences:

>Translated_610_residues
MVSNYNKRILIDGVYQDEIRVAVLRDGQVVEFDQESKSKKCLKGNIYCAVVKRIEPSLQAVFIEYGINKHGFLPFSEISL
EYYNIPEEEKETLYQTLYKDSDKDTDTKSDDEKSTDTMILSEDESEGIAESEEKLALLKKENSRNNYNKSKLPFHKLYKV
QDVIRLDQKIMVQIIKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIDDPEERKILKNFLNSIKLPKKSGLII
RTAGSNKARKEIEQDLSYLQSIWRSIQNSYISVTVPSLLYIEWDIIKRTLRDYCDESTQDIVVSGSHAYELAKECIKFMF
RNRVRLRIYKGSVPIFSYYKIESQICELYSNIVNLPSGGYMVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEI
ARQVNLRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAQVQFGYISTFGLMEISRQRVKQSILEANTVPCSHCKGV
GKVRSFESSSGDILRNIRYCANKNRNKLINVSVVAQMVEYFFNNKRQHITEIEAEFSVSIKLTVSQNLNSISDFSISVEN
DNIVNQSEDLVNVFTVDYKTSKSEDLKEEVQNSTSSVWIKRWLNRIFSHI
>Mature_610_residues
MVSNYNKRILIDGVYQDEIRVAVLRDGQVVEFDQESKSKKCLKGNIYCAVVKRIEPSLQAVFIEYGINKHGFLPFSEISL
EYYNIPEEEKETLYQTLYKDSDKDTDTKSDDEKSTDTMILSEDESEGIAESEEKLALLKKENSRNNYNKSKLPFHKLYKV
QDVIRLDQKIMVQIIKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIDDPEERKILKNFLNSIKLPKKSGLII
RTAGSNKARKEIEQDLSYLQSIWRSIQNSYISVTVPSLLYIEWDIIKRTLRDYCDESTQDIVVSGSHAYELAKECIKFMF
RNRVRLRIYKGSVPIFSYYKIESQICELYSNIVNLPSGGYMVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEI
ARQVNLRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAQVQFGYISTFGLMEISRQRVKQSILEANTVPCSHCKGV
GKVRSFESSSGDILRNIRYCANKNRNKLINVSVVAQMVEYFFNNKRQHITEIEAEFSVSIKLTVSQNLNSISDFSISVEN
DNIVNQSEDLVNVFTVDYKTSKSEDLKEEVQNSTSSVWIKRWLNRIFSHI

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=381, Percent_Identity=43.3070866141732, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI87082235, Length=347, Percent_Identity=34.0057636887608, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 70207; Mature: 70207

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSNYNKRILIDGVYQDEIRVAVLRDGQVVEFDQESKSKKCLKGNIYCAVVKRIEPSLQA
CCCCCCCEEEEECEECCCEEEEEEECCEEEEECCCCCCCHHHCCCEEEEEHHHCCCHHHE
VFIEYGINKHGFLPFSEISLEYYNIPEEEKETLYQTLYKDSDKDTDTKSDDEKSTDTMIL
EEEEECCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
SEDESEGIAESEEKLALLKKENSRNNYNKSKLPFHKLYKVQDVIRLDQKIMVQIIKEERG
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIDDPEERKILKNFLNSIKLPKKSGLII
CCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
RTAGSNKARKEIEQDLSYLQSIWRSIQNSYISVTVPSLLYIEWDIIKRTLRDYCDESTQD
EECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEHHHHHHHHHHHHCCCCCE
IVVSGSHAYELAKECIKFMFRNRVRLRIYKGSVPIFSYYKIESQICELYSNIVNLPSGGY
EEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCE
MVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEIARQVNLRGLSGLIVIDFIDM
EEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
LKYRYCRSVESEIKEAFKYDKAQVQFGYISTFGLMEISRQRVKQSILEANTVPCSHCKGV
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
GKVRSFESSSGDILRNIRYCANKNRNKLINVSVVAQMVEYFFNNKRQHITEIEAEFSVSI
CHHHCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEE
KLTVSQNLNSISDFSISVENDNIVNQSEDLVNVFTVDYKTSKSEDLKEEVQNSTSSVWIK
EEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHH
RWLNRIFSHI
HHHHHHHHCC
>Mature Secondary Structure
MVSNYNKRILIDGVYQDEIRVAVLRDGQVVEFDQESKSKKCLKGNIYCAVVKRIEPSLQA
CCCCCCCEEEEECEECCCEEEEEEECCEEEEECCCCCCCHHHCCCEEEEEHHHCCCHHHE
VFIEYGINKHGFLPFSEISLEYYNIPEEEKETLYQTLYKDSDKDTDTKSDDEKSTDTMIL
EEEEECCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
SEDESEGIAESEEKLALLKKENSRNNYNKSKLPFHKLYKVQDVIRLDQKIMVQIIKEERG
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIDDPEERKILKNFLNSIKLPKKSGLII
CCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
RTAGSNKARKEIEQDLSYLQSIWRSIQNSYISVTVPSLLYIEWDIIKRTLRDYCDESTQD
EECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEHHHHHHHHHHHHCCCCCE
IVVSGSHAYELAKECIKFMFRNRVRLRIYKGSVPIFSYYKIESQICELYSNIVNLPSGGY
EEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCE
MVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEIARQVNLRGLSGLIVIDFIDM
EEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
LKYRYCRSVESEIKEAFKYDKAQVQFGYISTFGLMEISRQRVKQSILEANTVPCSHCKGV
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
GKVRSFESSSGDILRNIRYCANKNRNKLINVSVVAQMVEYFFNNKRQHITEIEAEFSVSI
CHHHCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEE
KLTVSQNLNSISDFSISVENDNIVNQSEDLVNVFTVDYKTSKSEDLKEEVQNSTSSVWIK
EEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHH
RWLNRIFSHI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]