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The map label for this gene is lipA

Identifier: 58617271

GI number: 58617271

Start: 881202

End: 882080

Strand: Reverse

Name: lipA

Synonym: ERGA_CDS_05440

Alternate gene names: 58617271

Gene position: 882080-881202 (Counterclockwise)

Preceding gene: 58617272

Following gene: 58617269

Centisome position: 58.81

GC content: 33.67

Gene sequence:

>879_bases
ATGAAAAGTAAGCCTGATTGGTTAAAGGTAAAGATGCCTACAGGTAGTGCTTTTTATGAAATGCGCAATTTAATGAAGTT
GCATAAACTGAATACAGTATGTGAAGAAGCTGCATGTCCTAATATTGGTGAGTGTTGGAATAAGAAACATGCTACAGTTA
TGATTTTAGGTTCTACATGTACTAGAGCATGTGCATTTTGTAATGTTGCTTCTGGGATTCCTGATAAGTTGGATCCTCAT
GAGCCTCAAAGTTTGGCTAAAGCTGTTAGTTCGTTGAAGTTACAGCATGTTGTAATTACATCTGTGGATCGAGATGACTT
AGAAGATGGTGGTGCTGGGCATTTTGTAGAATGTATAGAAGAAATACGCAAAAGGGATAGTAATGTCACTATTGAAATAT
TAACTCCAGATTTTTTAAATAAACATGATGCAATTGATAAAATTGCTAAAGCATTTCCTGATGTGTATAATCATAATGTA
GAAACAGTACCAAGGCTGTATGCTAAGATTAGACCAAAAGCACGTTATTTTCATTCTTTATATTTGCTAAAAACAATAAA
GCAGAAAAACCCTAGAATATTTACTAAGTCAGGTATAATGGTAGGATTGGGAGAACTAAAAGAAGAGATATACCAAGTTA
TGGATGATTTAAGGTCGGCAGATGTTGATTTTATAGTAATTGGTCAATATTTACAGCCTACTTCTAAACATGCTGTAGTT
GATAGGTATGTTACTCCAGAGGAATTTGATCATTATAAATATGTTGCGTATTCCAAAGGATTTTTAATGGTTGCTTCTGG
TCCTTTAGTTAGATCTTCATATCATGCAGAAGAGGATTTTCAGAGACTTAAAAAAAATCGTGCTGCTATGTATACATAA

Upstream 100 bases:

>100_bases
TGTATTTATTATAGAGTTGGTAAGAAGTCATTTTCTTGACAGAATTGTAAGTATTAATTATCCTGAGTGGGTAGTAAAAC
TTGATTTCTAAATTTGCGAT

Downstream 100 bases:

>100_bases
GTATAAAGTGTGTAGTTAAAGGAAAGAAAAATATATATAGCTGTATACTATATTTTTAAAGTATCACTTATATAATCCAT
AACTTATAAGTTTTATTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKSKPDWLKVKMPTGSAFYEMRNLMKLHKLNTVCEEAACPNIGECWNKKHATVMILGSTCTRACAFCNVASGIPDKLDPH
EPQSLAKAVSSLKLQHVVITSVDRDDLEDGGAGHFVECIEEIRKRDSNVTIEILTPDFLNKHDAIDKIAKAFPDVYNHNV
ETVPRLYAKIRPKARYFHSLYLLKTIKQKNPRIFTKSGIMVGLGELKEEIYQVMDDLRSADVDFIVIGQYLQPTSKHAVV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQRLKKNRAAMYT

Sequences:

>Translated_292_residues
MKSKPDWLKVKMPTGSAFYEMRNLMKLHKLNTVCEEAACPNIGECWNKKHATVMILGSTCTRACAFCNVASGIPDKLDPH
EPQSLAKAVSSLKLQHVVITSVDRDDLEDGGAGHFVECIEEIRKRDSNVTIEILTPDFLNKHDAIDKIAKAFPDVYNHNV
ETVPRLYAKIRPKARYFHSLYLLKTIKQKNPRIFTKSGIMVGLGELKEEIYQVMDDLRSADVDFIVIGQYLQPTSKHAVV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQRLKKNRAAMYT
>Mature_292_residues
MKSKPDWLKVKMPTGSAFYEMRNLMKLHKLNTVCEEAACPNIGECWNKKHATVMILGSTCTRACAFCNVASGIPDKLDPH
EPQSLAKAVSSLKLQHVVITSVDRDDLEDGGAGHFVECIEEIRKRDSNVTIEILTPDFLNKHDAIDKIAKAFPDVYNHNV
ETVPRLYAKIRPKARYFHSLYLLKTIKQKNPRIFTKSGIMVGLGELKEEIYQVMDDLRSADVDFIVIGQYLQPTSKHAVV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQRLKKNRAAMYT

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=47.3498233215548, Blast_Score=277, Evalue=9e-75,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=46.3114754098361, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=51.4492753623188, Blast_Score=297, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=47.244094488189, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=46.5034965034965, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=48.4098939929329, Blast_Score=277, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_EHRRG (Q5FFK4)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196470.1
- ProteinModelPortal:   Q5FFK4
- SMR:   Q5FFK4
- STRING:   Q5FFK4
- GeneID:   3268613
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_05440
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   ERUM302409:ERGA_CDS_05440-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33220; Mature: 33220

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKPDWLKVKMPTGSAFYEMRNLMKLHKLNTVCEEAACPNIGECWNKKHATVMILGSTC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCHH
TRACAFCNVASGIPDKLDPHEPQSLAKAVSSLKLQHVVITSVDRDDLEDGGAGHFVECIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCHHCCCCCCCCHHHHHHH
EIRKRDSNVTIEILTPDFLNKHDAIDKIAKAFPDVYNHNVETVPRLYAKIRPKARYFHSL
HHHHCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHH
YLLKTIKQKNPRIFTKSGIMVGLGELKEEIYQVMDDLRSADVDFIVIGQYLQPTSKHAVV
HHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHH
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQRLKKNRAAMYT
HCCCCHHHHCCEEEEEEECCEEEEECCCHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSKPDWLKVKMPTGSAFYEMRNLMKLHKLNTVCEEAACPNIGECWNKKHATVMILGSTC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCHH
TRACAFCNVASGIPDKLDPHEPQSLAKAVSSLKLQHVVITSVDRDDLEDGGAGHFVECIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCHHCCCCCCCCHHHHHHH
EIRKRDSNVTIEILTPDFLNKHDAIDKIAKAFPDVYNHNVETVPRLYAKIRPKARYFHSL
HHHHCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHH
YLLKTIKQKNPRIFTKSGIMVGLGELKEEIYQVMDDLRSADVDFIVIGQYLQPTSKHAVV
HHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHH
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQRLKKNRAAMYT
HCCCCHHHHCCEEEEEEECCEEEEECCCHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA