Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 58617212

GI number: 58617212

Start: 785697

End: 786965

Strand: Reverse

Name: ispG

Synonym: ERGA_CDS_04850

Alternate gene names: 58617212

Gene position: 786965-785697 (Counterclockwise)

Preceding gene: 58617213

Following gene: 58617211

Centisome position: 52.47

GC content: 32.39

Gene sequence:

>1269_bases
ATGCAGTGTGATTTTGTGGACAGAATTTTAAATGAATATGATTTGCTAAACGCTATTGTATACAGAAGTAAATTCACTCA
TGAAGTTAAAGTGGGTGATGTGATAATTGGTGGTAATAATCCTATAGTTGTACAGTCAATGGCGCTTGGTGGTTCTGGAG
ATGTTTATCAAGATGCACGTGAAGTATTAGAACTTGCACAGGCTGGGTCTGAATTAGTAAGAATTGCAATTAATTCAGAT
AAAGCAATAAAAAGTGTTCCTTATATTAGAGATGTATTAATTAATCATGGTTTTAATAGTAAAATGATCATAGGTTGTGG
ACAGTATGAGATTGCTAGATTAGTAAAACAATATCCAGAATGTGCACTTGCTTTAGGGAAAATACGGATTAATCCAGGAA
ATATAGGTTTTGGAGATAAAAGAGATAAAAATTTTGAAGATATTATTGAGTTTGCAATAAAAAATGATATACCTATCAGA
ATAGGAGTTAACTGGGGAAGTTTAGATAAGTATTTGGCTGCAAAATTGATGCATGATAATTCATTACGTGGTACTCCTGA
TCCAGATTATGTAGTTTTAAGGAAGGCATTAGTAATTTCTGCTATAACAAGTGCTAAACATGCAGAAAAAGTTGGTTTGC
CTGCGAATAAAATAGTTATATCTTGTAAAGTGAGTAAAGTACGTGACTTAATATCTGTATATACTATGTTGTCAAAGATT
TGTGATTATCCTCTGCATTTAGGCTTGACAGAAGCTGGATCTGGTGTAAAAGGAATTGTTGGTAGTTCAGCTGGAATATC
TTATCTTTTACTTCATGGTATAGGTAATACTATTAGGGTTTCATTAACTCAACAGCCTGGAGAATCAAGAACTAATGAAG
TAAAATTGTGTCAAGAAATTCTCCAAAGTATAGGGTTAAGAAATTTTTCTGTACAAGTTACTTCATGTCCAGGATGTAAT
AGGACTAATCCAAAGTATTTTCATCAATTAGTTAGTGATGTGAATAAATATGTAGCAGATCGTATGAGTGTATGGAAAAG
TGCAAATCCTGGAGTTGAGAATATGACTATAGCAGTTATGGGCTGTGTAGTTAATGGGCCTGGTGAAAGCAAGCATGCTA
ATTTAGGAATTAGTATGCCTGGTTATGGTGAAAGATCTGTAGCTGCTGTATATCAAAATGGTGAAAAATTATGTACTTTA
GAAGGTAATAATATTTTTGAGCAATTTGTATCAATTATTGAAAATTATGTTAGTATTTATTATCACTGA

Upstream 100 bases:

>100_bases
CTTTATTAAGTTAGAATAATGCTATTATTAGCAGAATAGAAGATTTGTTTAATCTTTATATTATGTTATAGTTACGTCTG
GTTGTTGTAGTAGGTTTGAT

Downstream 100 bases:

>100_bases
TTAAAAAGTGCAAAGGTAATTTGTATAATGAAATCTACAAATGGTGAAATGCCCTTTTTTAAGCATTTTGAGGAATTAAG
ATATAGGATATTCTTTTGTT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQCDFVDRILNEYDLLNAIVYRSKFTHEVKVGDVIIGGNNPIVVQSMALGGSGDVYQDAREVLELAQAGSELVRIAINSD
KAIKSVPYIRDVLINHGFNSKMIIGCGQYEIARLVKQYPECALALGKIRINPGNIGFGDKRDKNFEDIIEFAIKNDIPIR
IGVNWGSLDKYLAAKLMHDNSLRGTPDPDYVVLRKALVISAITSAKHAEKVGLPANKIVISCKVSKVRDLISVYTMLSKI
CDYPLHLGLTEAGSGVKGIVGSSAGISYLLLHGIGNTIRVSLTQQPGESRTNEVKLCQEILQSIGLRNFSVQVTSCPGCN
RTNPKYFHQLVSDVNKYVADRMSVWKSANPGVENMTIAVMGCVVNGPGESKHANLGISMPGYGERSVAAVYQNGEKLCTL
EGNNIFEQFVSIIENYVSIYYH

Sequences:

>Translated_422_residues
MQCDFVDRILNEYDLLNAIVYRSKFTHEVKVGDVIIGGNNPIVVQSMALGGSGDVYQDAREVLELAQAGSELVRIAINSD
KAIKSVPYIRDVLINHGFNSKMIIGCGQYEIARLVKQYPECALALGKIRINPGNIGFGDKRDKNFEDIIEFAIKNDIPIR
IGVNWGSLDKYLAAKLMHDNSLRGTPDPDYVVLRKALVISAITSAKHAEKVGLPANKIVISCKVSKVRDLISVYTMLSKI
CDYPLHLGLTEAGSGVKGIVGSSAGISYLLLHGIGNTIRVSLTQQPGESRTNEVKLCQEILQSIGLRNFSVQVTSCPGCN
RTNPKYFHQLVSDVNKYVADRMSVWKSANPGVENMTIAVMGCVVNGPGESKHANLGISMPGYGERSVAAVYQNGEKLCTL
EGNNIFEQFVSIIENYVSIYYH
>Mature_422_residues
MQCDFVDRILNEYDLLNAIVYRSKFTHEVKVGDVIIGGNNPIVVQSMALGGSGDVYQDAREVLELAQAGSELVRIAINSD
KAIKSVPYIRDVLINHGFNSKMIIGCGQYEIARLVKQYPECALALGKIRINPGNIGFGDKRDKNFEDIIEFAIKNDIPIR
IGVNWGSLDKYLAAKLMHDNSLRGTPDPDYVVLRKALVISAITSAKHAEKVGLPANKIVISCKVSKVRDLISVYTMLSKI
CDYPLHLGLTEAGSGVKGIVGSSAGISYLLLHGIGNTIRVSLTQQPGESRTNEVKLCQEILQSIGLRNFSVQVTSCPGCN
RTNPKYFHQLVSDVNKYVADRMSVWKSANPGVENMTIAVMGCVVNGPGESKHANLGISMPGYGERSVAAVYQNGEKLCTL
EGNNIFEQFVSIIENYVSIYYH

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=404, Percent_Identity=31.4356435643564, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_EHRRG (Q5FHA6)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196411.1
- ProteinModelPortal:   Q5FHA6
- STRING:   Q5FHA6
- GeneID:   3268622
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_04850
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   ERUM302409:ERGA_CDS_04850-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 46204; Mature: 46204

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCDFVDRILNEYDLLNAIVYRSKFTHEVKVGDVIIGGNNPIVVQSMALGGSGDVYQDAR
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECEEEECCCCCEEEEEEECCCCCCHHHHHH
EVLELAQAGSELVRIAINSDKAIKSVPYIRDVLINHGFNSKMIIGCGQYEIARLVKQYPE
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCH
CALALGKIRINPGNIGFGDKRDKNFEDIIEFAIKNDIPIRIGVNWGSLDKYLAAKLMHDN
HHEEECEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCC
SLRGTPDPDYVVLRKALVISAITSAKHAEKVGLPANKIVISCKVSKVRDLISVYTMLSKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
CDYPLHLGLTEAGSGVKGIVGSSAGISYLLLHGIGNTIRVSLTQQPGESRTNEVKLCQEI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHHHH
LQSIGLRNFSVQVTSCPGCNRTNPKYFHQLVSDVNKYVADRMSVWKSANPGVENMTIAVM
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GCVVNGPGESKHANLGISMPGYGERSVAAVYQNGEKLCTLEGNNIFEQFVSIIENYVSIY
EEEECCCCCCCCCEEECCCCCCCCCHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHHC
YH
CC
>Mature Secondary Structure
MQCDFVDRILNEYDLLNAIVYRSKFTHEVKVGDVIIGGNNPIVVQSMALGGSGDVYQDAR
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECEEEECCCCCEEEEEEECCCCCCHHHHHH
EVLELAQAGSELVRIAINSDKAIKSVPYIRDVLINHGFNSKMIIGCGQYEIARLVKQYPE
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCH
CALALGKIRINPGNIGFGDKRDKNFEDIIEFAIKNDIPIRIGVNWGSLDKYLAAKLMHDN
HHEEECEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCC
SLRGTPDPDYVVLRKALVISAITSAKHAEKVGLPANKIVISCKVSKVRDLISVYTMLSKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
CDYPLHLGLTEAGSGVKGIVGSSAGISYLLLHGIGNTIRVSLTQQPGESRTNEVKLCQEI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHHHH
LQSIGLRNFSVQVTSCPGCNRTNPKYFHQLVSDVNKYVADRMSVWKSANPGVENMTIAVM
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GCVVNGPGESKHANLGISMPGYGERSVAAVYQNGEKLCTLEGNNIFEQFVSIIENYVSIY
EEEECCCCCCCCCEEECCCCCCCCCHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHHC
YH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA