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The map label for this gene is gltX

Identifier: 58617169

GI number: 58617169

Start: 718026

End: 719438

Strand: Reverse

Name: gltX

Synonym: ERGA_CDS_04420

Alternate gene names: 58617169

Gene position: 719438-718026 (Counterclockwise)

Preceding gene: 58617170

Following gene: 58617164

Centisome position: 47.97

GC content: 28.24

Gene sequence:

>1413_bases
ATGCTTAATCATGTAGTTACTAGATTTGCACCATCACCTACTGGACATTTGCATTTAGGTGGTGCAAGAACTGCTCTGTT
TAACTGGTTATATGCTAAACACAATAATGGTAAATTTTTATTACGAATTGAAGATACTGATAGTAAGAGGTCATCTAAGG
AATTAATAGATTCAATTATTAATTCAATGATTTGGCTAAATATCCAGCATGATGGAGAGATTATATTACAATCGAGCAGA
ATATCAAGACATATTGAAATTGCAAATCAATTGATATTGAATGATAAAGCATATTATTGCTATTGCTCTGAAGAAGAAAT
TAATTTGGAAAAAGAACAATATACGAAAAAAGGATTACATTATAAACATAATTGTATATGGAAAAATAGAAAACCACCTA
CTGGTAATTGTAGTAAAGTAATTAGATTACATAGTGATACAGAAGGTATAACTGAGTTTAAAGATAAGGTTTATGGTACT
ATTGCAGTTAATAATGTACAACTTGATGATATGGTGTTATTGCGATCTAATAATACTCCAACATATCTTTTATCTGTTGT
TGTTGATGATTATGATATGGGTATTACACATATTATTAGAGGAACAGACCACTTGACTAATACAGCACGTCAACTATTAA
TATATAATGCATTGGGTTGGAAACCTCCTGAATTTGCTCATATTCCATTAATACATGATGAAAACGGGAATAAATTATCT
AAAAGGCATCATGCATTAGGTATACATGAATATAAGAATGCTGGAATATTACCAGAAGCTTTGGTAAACTACTTATTAAG
GATGGGGTGGAGCCATGGCAATGATGAACTTATTACTATTGATGAAGCCATACGTTGGTTTTCCATAGATAAAGTAGGAC
AATCTCCAGCAGGGTTAGATAGCAAAAAACTAGAATTTTTAAATAATCACTATATTAATACAACTAATAATGCAACTATT
ATTGAGATGATAATTCCTATCATAGAGAAAACGATAGGATATAAAGTCAATACAGAGAAAATAGGTTATCTATTAAATGG
AATTAATGAACTAAAAAAGAGAACAAAAAATTTAGTTAATTTAGCAAATGAGTCTCTATTTTATGTTGAAGATATTCCAA
TTTCTTTTGATCAAGAATCTCTTATAATTATCAAATCTAACCATGATATATTAAGCATTTTATATGATAATCTATCAAAA
GTTTTAAATGATGACTGGAATAACAGTACATTAACTTCAATGATCAAAAATATTGTAAAAGATTACAATACAAAAATTCA
TAATATTTATCACTGTTTACGTGCATCTATTATCGGAAGAATAAATGCTCCAAGTATCATAGATATAATGGTTAATTTTC
AGCGTGAAGAATGCTTAAATAGAATAAAATATGCAAGAGATATGGTAAAATAA

Upstream 100 bases:

>100_bases
ATGGTATACAATTGTTATTTTGGTATTTCACGATAGATATTTAGCTTTACAAAATTGTTTTTATATTAAACAATGAAAAA
TTTTAAAATTTCTTTTTTTT

Downstream 100 bases:

>100_bases
TACTTTATTTTAAGGATAAAAAATTATAAGGTAACAAATAGTATTAGTGTATGGCTTACTATATAACTAAATTTTTGTTT
TTTCTATCAAAGTTAGTGCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLNHVVTRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDSKRSSKELIDSIINSMIWLNIQHDGEIILQSSR
ISRHIEIANQLILNDKAYYCYCSEEEINLEKEQYTKKGLHYKHNCIWKNRKPPTGNCSKVIRLHSDTEGITEFKDKVYGT
IAVNNVQLDDMVLLRSNNTPTYLLSVVVDDYDMGITHIIRGTDHLTNTARQLLIYNALGWKPPEFAHIPLIHDENGNKLS
KRHHALGIHEYKNAGILPEALVNYLLRMGWSHGNDELITIDEAIRWFSIDKVGQSPAGLDSKKLEFLNNHYINTTNNATI
IEMIIPIIEKTIGYKVNTEKIGYLLNGINELKKRTKNLVNLANESLFYVEDIPISFDQESLIIIKSNHDILSILYDNLSK
VLNDDWNNSTLTSMIKNIVKDYNTKIHNIYHCLRASIIGRINAPSIIDIMVNFQREECLNRIKYARDMVK

Sequences:

>Translated_470_residues
MLNHVVTRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDSKRSSKELIDSIINSMIWLNIQHDGEIILQSSR
ISRHIEIANQLILNDKAYYCYCSEEEINLEKEQYTKKGLHYKHNCIWKNRKPPTGNCSKVIRLHSDTEGITEFKDKVYGT
IAVNNVQLDDMVLLRSNNTPTYLLSVVVDDYDMGITHIIRGTDHLTNTARQLLIYNALGWKPPEFAHIPLIHDENGNKLS
KRHHALGIHEYKNAGILPEALVNYLLRMGWSHGNDELITIDEAIRWFSIDKVGQSPAGLDSKKLEFLNNHYINTTNNATI
IEMIIPIIEKTIGYKVNTEKIGYLLNGINELKKRTKNLVNLANESLFYVEDIPISFDQESLIIIKSNHDILSILYDNLSK
VLNDDWNNSTLTSMIKNIVKDYNTKIHNIYHCLRASIIGRINAPSIIDIMVNFQREECLNRIKYARDMVK
>Mature_470_residues
MLNHVVTRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDSKRSSKELIDSIINSMIWLNIQHDGEIILQSSR
ISRHIEIANQLILNDKAYYCYCSEEEINLEKEQYTKKGLHYKHNCIWKNRKPPTGNCSKVIRLHSDTEGITEFKDKVYGT
IAVNNVQLDDMVLLRSNNTPTYLLSVVVDDYDMGITHIIRGTDHLTNTARQLLIYNALGWKPPEFAHIPLIHDENGNKLS
KRHHALGIHEYKNAGILPEALVNYLLRMGWSHGNDELITIDEAIRWFSIDKVGQSPAGLDSKKLEFLNNHYINTTNNATI
IEMIIPIIEKTIGYKVNTEKIGYLLNGINELKKRTKNLVNLANESLFYVEDIPISFDQESLIIIKSNHDILSILYDNLSK
VLNDDWNNSTLTSMIKNIVKDYNTKIHNIYHCLRASIIGRINAPSIIDIMVNFQREECLNRIKYARDMVK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=501, Percent_Identity=29.3413173652695, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI62241042, Length=260, Percent_Identity=25.3846153846154, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=34.4017094017094, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI226510925, Length=291, Percent_Identity=31.2714776632302, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=30.7535641547862, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=464, Percent_Identity=32.1120689655172, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=120, Percent_Identity=36.6666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=232, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=504, Percent_Identity=29.5634920634921, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_EHRRG (Q5FGW4)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196368.1
- ProteinModelPortal:   Q5FGW4
- SMR:   Q5FGW4
- STRING:   Q5FGW4
- GeneID:   3268757
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_04420
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NGINELK
- ProtClustDB:   PRK01406
- BioCyc:   ERUM302409:ERGA_CDS_04420-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54028; Mature: 54028

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNHVVTRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDSKRSSKELIDSII
CCCCHHHHCCCCCCCEEEECCHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHH
NSMIWLNIQHDGEIILQSSRISRHIEIANQLILNDKAYYCYCSEEEINLEKEQYTKKGLH
CCEEEEEEECCCCEEEECCHHHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHCCCC
YKHNCIWKNRKPPTGNCSKVIRLHSDTEGITEFKDKVYGTIAVNNVQLDDMVLLRSNNTP
EECCEEECCCCCCCCCHHHEEEEECCCCHHHHHHHHEEEEEEECCEEECCEEEEECCCCC
TYLLSVVVDDYDMGITHIIRGTDHLTNTARQLLIYNALGWKPPEFAHIPLIHDENGNKLS
EEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHH
KRHHALGIHEYKNAGILPEALVNYLLRMGWSHGNDELITIDEAIRWFSIDKVGQSPAGLD
HHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHEEHHHCCCCCCCCC
SKKLEFLNNHYINTTNNATIIEMIIPIIEKTIGYKVNTEKIGYLLNGINELKKRTKNLVN
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHH
LANESLFYVEDIPISFDQESLIIIKSNHDILSILYDNLSKVLNDDWNNSTLTSMIKNIVK
HHCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DYNTKIHNIYHCLRASIIGRINAPSIIDIMVNFQREECLNRIKYARDMVK
HHCHHHHHHHHHHHHHHHCCCCCCCEEEHEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNHVVTRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDSKRSSKELIDSII
CCCCHHHHCCCCCCCEEEECCHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHH
NSMIWLNIQHDGEIILQSSRISRHIEIANQLILNDKAYYCYCSEEEINLEKEQYTKKGLH
CCEEEEEEECCCCEEEECCHHHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHCCCC
YKHNCIWKNRKPPTGNCSKVIRLHSDTEGITEFKDKVYGTIAVNNVQLDDMVLLRSNNTP
EECCEEECCCCCCCCCHHHEEEEECCCCHHHHHHHHEEEEEEECCEEECCEEEEECCCCC
TYLLSVVVDDYDMGITHIIRGTDHLTNTARQLLIYNALGWKPPEFAHIPLIHDENGNKLS
EEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHH
KRHHALGIHEYKNAGILPEALVNYLLRMGWSHGNDELITIDEAIRWFSIDKVGQSPAGLD
HHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHEEHHHCCCCCCCCC
SKKLEFLNNHYINTTNNATIIEMIIPIIEKTIGYKVNTEKIGYLLNGINELKKRTKNLVN
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHH
LANESLFYVEDIPISFDQESLIIIKSNHDILSILYDNLSKVLNDDWNNSTLTSMIKNIVK
HHCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DYNTKIHNIYHCLRASIIGRINAPSIIDIMVNFQREECLNRIKYARDMVK
HHCHHHHHHHHHHHHHHHCCCCCCCEEEHEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA