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The map label for this gene is truA

Identifier: 58617163

GI number: 58617163

Start: 708431

End: 709171

Strand: Reverse

Name: truA

Synonym: ERGA_CDS_04360

Alternate gene names: 58617163

Gene position: 709171-708431 (Counterclockwise)

Preceding gene: 58617164

Following gene: 58617150

Centisome position: 47.28

GC content: 29.28

Gene sequence:

>741_bases
ATGCGATATAAAATTGTTATAGAGTATGATGGTTCCAATTTTATTGGATGGCAGAAACAAAATCATAATTCTAATTCTAT
TCAAGAAATACTAGAAAAAGCTATATTTAAATTTTCCAAACAGCATGTTACAGTATATGGAGCAGGCAGAACAGATGCAG
GTGTACATGCATTAGGCCAAGTAGCACATTTTGATTTAACAACAGATTTTGAAACATATATTGTCAGAAATGCAATAAAT
TACCATTTGATATCTCATGCTATTGCTGTAGTGCATGTAGAAAAAACAGATACTGATTTTCATGCAAGATTTTCAGCAAA
AAGACGATATTACTTATATAAAATAGTAAATCGTTACTCACCTTTAACAATAGACCGTAATAGAGCATGGTTAGTACATA
CTCCTTTAAACGTTGAAAATATGATAAAAGCAGTATGTTATATTAAAGGAAATCATAATTTTTCTAGTTTTCGTGCTAAA
TGTTGTCAATCTAAAAGTCCTGTAAAAACAGTAGATAATTTAAGTATTACATATAATCATCCTTATATTGACATTAATAT
ATCTGCTATATCTTTTTTACATCATCAGGTTCGCATTATTGTTGGAACATTAGTAGAATGTGGAAAAGGTTACTTTCCAC
CAGAGCATATTAAAACTATCATGGAAGCGAATAATAGATCTTATGCTGGTACAACAGCTCCATCTTATGGATTATATTTT
GTAAAAGTAGATTACTCTTAA

Upstream 100 bases:

>100_bases
ATTTGTTCAAAAAAATTACCACGCAAAAAAATGGGTATTGCAGTATAAGAATTAATATTACAGTATTTAGTGTGCAATAA
TTAAGAAAAAACATTAAATA

Downstream 100 bases:

>100_bases
ATACAAAAATATACAGTTTAATTGTATGTAAAATTGCTAAGGCTATTATGATTTTTCTCCATTTTTTATAATAACCTTAT
AAGAAACATGCTAAAGTATG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRYKIVIEYDGSNFIGWQKQNHNSNSIQEILEKAIFKFSKQHVTVYGAGRTDAGVHALGQVAHFDLTTDFETYIVRNAIN
YHLISHAIAVVHVEKTDTDFHARFSAKRRYYLYKIVNRYSPLTIDRNRAWLVHTPLNVENMIKAVCYIKGNHNFSSFRAK
CCQSKSPVKTVDNLSITYNHPYIDINISAISFLHHQVRIIVGTLVECGKGYFPPEHIKTIMEANNRSYAGTTAPSYGLYF
VKVDYS

Sequences:

>Translated_246_residues
MRYKIVIEYDGSNFIGWQKQNHNSNSIQEILEKAIFKFSKQHVTVYGAGRTDAGVHALGQVAHFDLTTDFETYIVRNAIN
YHLISHAIAVVHVEKTDTDFHARFSAKRRYYLYKIVNRYSPLTIDRNRAWLVHTPLNVENMIKAVCYIKGNHNFSSFRAK
CCQSKSPVKTVDNLSITYNHPYIDINISAISFLHHQVRIIVGTLVECGKGYFPPEHIKTIMEANNRSYAGTTAPSYGLYF
VKVDYS
>Mature_246_residues
MRYKIVIEYDGSNFIGWQKQNHNSNSIQEILEKAIFKFSKQHVTVYGAGRTDAGVHALGQVAHFDLTTDFETYIVRNAIN
YHLISHAIAVVHVEKTDTDFHARFSAKRRYYLYKIVNRYSPLTIDRNRAWLVHTPLNVENMIKAVCYIKGNHNFSSFRAK
CCQSKSPVKTVDNLSITYNHPYIDINISAISFLHHQVRIIVGTLVECGKGYFPPEHIKTIMEANNRSYAGTTAPSYGLYF
VKVDYS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=30.5147058823529, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=42.6829268292683, Blast_Score=182, Evalue=1e-47,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=28.9036544850498, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_EHRRG (Q5FGS1)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196362.1
- ProteinModelPortal:   Q5FGS1
- SMR:   Q5FGS1
- STRING:   Q5FGS1
- GeneID:   3268381
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_04360
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   TIMEANN
- ProtClustDB:   PRK00021
- BioCyc:   ERUM302409:ERGA_CDS_04360-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28245; Mature: 28245

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKIVIEYDGSNFIGWQKQNHNSNSIQEILEKAIFKFSKQHVTVYGAGRTDAGVHALGQ
CEEEEEEEECCCCEECEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
VAHFDLTTDFETYIVRNAINYHLISHAIAVVHVEKTDTDFHARFSAKRRYYLYKIVNRYS
EEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHEEECCCHHHHEEEHHHCCC
PLTIDRNRAWLVHTPLNVENMIKAVCYIKGNHNFSSFRAKCCQSKSPVKTVDNLSITYNH
CEEEECCCEEEEECCCCHHHHEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECC
PYIDINISAISFLHHQVRIIVGTLVECGKGYFPPEHIKTIMEANNRSYAGTTAPSYGLYF
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEE
VKVDYS
EEEECC
>Mature Secondary Structure
MRYKIVIEYDGSNFIGWQKQNHNSNSIQEILEKAIFKFSKQHVTVYGAGRTDAGVHALGQ
CEEEEEEEECCCCEECEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
VAHFDLTTDFETYIVRNAINYHLISHAIAVVHVEKTDTDFHARFSAKRRYYLYKIVNRYS
EEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHEEECCCHHHHEEEHHHCCC
PLTIDRNRAWLVHTPLNVENMIKAVCYIKGNHNFSSFRAKCCQSKSPVKTVDNLSITYNH
CEEEECCCEEEEECCCCHHHHEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECC
PYIDINISAISFLHHQVRIIVGTLVECGKGYFPPEHIKTIMEANNRSYAGTTAPSYGLYF
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEE
VKVDYS
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA