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The map label for this gene is gatA [H]

Identifier: 58617100

GI number: 58617100

Start: 616564

End: 618066

Strand: Reverse

Name: gatA [H]

Synonym: ERGA_CDS_03730

Alternate gene names: 58617100

Gene position: 618066-616564 (Counterclockwise)

Preceding gene: 58617106

Following gene: 58617099

Centisome position: 41.21

GC content: 34.4

Gene sequence:

>1503_bases
ATGGTAATTTACTGCCACTCTGATCTATTTCACTCAAATATGAAAGATATTTTAAAATTATCCATTGCAGAAATGCATGA
AAATCTAATAAAAAAAGAGTTCTCAGCTGTAGAACTCACCCAAGCTCATATTGATGCTATAAACAATGAACAGTTAAATG
CTTTTATTACAAAAACACCAGAAGTTGCATTAAATGCTGCCAGAAAAGCAGATCACATTTTAACACATGAACAAGACAAA
ATTACCTCATTAACTGGAATCCCAGTTGGAATAAAAGACTTATTTTGTACAAAAAATATTAAAACAACAGCATGTTCTAA
TATACTAAGGAATTTTACTCCACAATATGACTCAACTGTAGTAAAGCGACTTATTGATGATGGTGCTGTAATGCTAGGAA
AGCTAAATATGGATGAATTTGCTATGGGTTCATCAAATTCTAATAGCTGCTTTGGTCCTGTAGAAAATCCCTGGACAAGA
GCAGATGGAGTAAAAGTAGTACCTGGAGGATCATCCGGTGGATCAAGTGCTGCTGTAGCAGGTTTTCTATGTGCTGGCGC
TTTAGGAAGTGATACAGGTGGATCAGTACGTCAACCTGCAGCATTCTGTGGAATAGTAGGATTAAAACCAACATATGGGA
GGTGTTCTAGATCAGGTATGATAGCATTTGCAAGTTCTCTTGATCAAGCAGGAGTACTAACACGCACAGTAAAAGATGCA
GCTCTAATGCTACAACCAATTTGTGGATATGATACTCAAGATTCCACTTCTGCTAACATAACCACACCCAGGTTCCTAGA
TAGTATTACAAACATCATAAAAGGTAAACGAATTGGAATTCCTAAAGAATATGAGTTATCAAATAAGTATAAAGAATACG
AGGAAGTTTCAGAGATGTGGTTAAAGGGAATTAAGTACCTAGAAAATGAAGGAGCCGAAATTGTAAATATTAGTTTGCCA
CACACAAGTTATGCACTTCCCGTATATTACATTATTTGCTCTGCAGAAGCATCATCTAACCTTGCAAGATATGATGGAAT
AAAATACGGAACACGTGTAAACTCTGATAACATAAATGAAATGTATGAGTTAACACGAGGAAATAATCTTGGGACAGAAG
TAAAAAGAAGAATATTAATAGGAGCATACGCATTGTCATCTGGATATTATGATGCATATTATAACAAAGCACAATGTATT
AGACGTTTAGTAACAAATGATTTTGTCGAATCCTTTAAGAGTGTTGATTACATTCTCACCCCTACTGCTCCTAAAGAAGC
GTTCGCAATGGATGAACAATTAGATACATTAACTATGTATTTAAATGACGTATTTACTGTTCCAGCAAGTCTAGCTGGAT
TGCCCGCTATTTCTATACCAATAGGATTATCAAAAAACAAGTTACCTCTTTCTTTACAGATAATAGGTAACTATTATGAT
GAAGGAGGTATTTTAAATATAGCAAGTGTCATAGAAAAATACACAGGAAATATTTTAAAATAA

Upstream 100 bases:

>100_bases
AAGAGCTTTTTATATTGTAGCAACATAGATAATTATTCTGATATATACAACTAACACTATAAGTATTACCAAAAAATTTA
ATTGTTTTATAATAGATAAT

Downstream 100 bases:

>100_bases
AGGTTATATAAACATGGCTGGTCATTCACAATTTGCAAATATAAAACATCGTAAGGGAGCTCAAGATGCAAAAAGAGCTC
AAAAATTTACCAAATTAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MVIYCHSDLFHSNMKDILKLSIAEMHENLIKKEFSAVELTQAHIDAINNEQLNAFITKTPEVALNAARKADHILTHEQDK
ITSLTGIPVGIKDLFCTKNIKTTACSNILRNFTPQYDSTVVKRLIDDGAVMLGKLNMDEFAMGSSNSNSCFGPVENPWTR
ADGVKVVPGGSSGGSSAAVAGFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRSGMIAFASSLDQAGVLTRTVKDA
ALMLQPICGYDTQDSTSANITTPRFLDSITNIIKGKRIGIPKEYELSNKYKEYEEVSEMWLKGIKYLENEGAEIVNISLP
HTSYALPVYYIICSAEASSNLARYDGIKYGTRVNSDNINEMYELTRGNNLGTEVKRRILIGAYALSSGYYDAYYNKAQCI
RRLVTNDFVESFKSVDYILTPTAPKEAFAMDEQLDTLTMYLNDVFTVPASLAGLPAISIPIGLSKNKLPLSLQIIGNYYD
EGGILNIASVIEKYTGNILK

Sequences:

>Translated_500_residues
MVIYCHSDLFHSNMKDILKLSIAEMHENLIKKEFSAVELTQAHIDAINNEQLNAFITKTPEVALNAARKADHILTHEQDK
ITSLTGIPVGIKDLFCTKNIKTTACSNILRNFTPQYDSTVVKRLIDDGAVMLGKLNMDEFAMGSSNSNSCFGPVENPWTR
ADGVKVVPGGSSGGSSAAVAGFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRSGMIAFASSLDQAGVLTRTVKDA
ALMLQPICGYDTQDSTSANITTPRFLDSITNIIKGKRIGIPKEYELSNKYKEYEEVSEMWLKGIKYLENEGAEIVNISLP
HTSYALPVYYIICSAEASSNLARYDGIKYGTRVNSDNINEMYELTRGNNLGTEVKRRILIGAYALSSGYYDAYYNKAQCI
RRLVTNDFVESFKSVDYILTPTAPKEAFAMDEQLDTLTMYLNDVFTVPASLAGLPAISIPIGLSKNKLPLSLQIIGNYYD
EGGILNIASVIEKYTGNILK
>Mature_500_residues
MVIYCHSDLFHSNMKDILKLSIAEMHENLIKKEFSAVELTQAHIDAINNEQLNAFITKTPEVALNAARKADHILTHEQDK
ITSLTGIPVGIKDLFCTKNIKTTACSNILRNFTPQYDSTVVKRLIDDGAVMLGKLNMDEFAMGSSNSNSCFGPVENPWTR
ADGVKVVPGGSSGGSSAAVAGFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRSGMIAFASSLDQAGVLTRTVKDA
ALMLQPICGYDTQDSTSANITTPRFLDSITNIIKGKRIGIPKEYELSNKYKEYEEVSEMWLKGIKYLENEGAEIVNISLP
HTSYALPVYYIICSAEASSNLARYDGIKYGTRVNSDNINEMYELTRGNNLGTEVKRRILIGAYALSSGYYDAYYNKAQCI
RRLVTNDFVESFKSVDYILTPTAPKEAFAMDEQLDTLTMYLNDVFTVPASLAGLPAISIPIGLSKNKLPLSLQIIGNYYD
EGGILNIASVIEKYTGNILK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=42.6587301587302, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=24.3512974051896, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI166795287, Length=137, Percent_Identity=36.4963503649635, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=37.3873873873874, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=26.2745098039216, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=280, Percent_Identity=28.2142857142857, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=160, Percent_Identity=34.375, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=249, Percent_Identity=30.5220883534137, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=249, Percent_Identity=30.5220883534137, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=36.1471861471861, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=444, Percent_Identity=25.2252252252252, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=213, Percent_Identity=29.1079812206573, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=42.0523138832998, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=25.2427184466019, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652985, Length=518, Percent_Identity=25.2895752895753, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922090, Length=518, Percent_Identity=25.2895752895753, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652981, Length=518, Percent_Identity=25.2895752895753, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652983, Length=518, Percent_Identity=25.2895752895753, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644968, Length=249, Percent_Identity=30.1204819277108, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356731, Length=498, Percent_Identity=23.0923694779116, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=265, Percent_Identity=29.0566037735849, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=33.5227272727273, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=33.5227272727273, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54573; Mature: 54573

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIYCHSDLFHSNMKDILKLSIAEMHENLIKKEFSAVELTQAHIDAINNEQLNAFITKTP
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
EVALNAARKADHILTHEQDKITSLTGIPVGIKDLFCTKNIKTTACSNILRNFTPQYDSTV
HHHHHHHHHHHHHHCCCCHHHHEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
VKRLIDDGAVMLGKLNMDEFAMGSSNSNSCFGPVENPWTRADGVKVVPGGSSGGSSAAVA
HHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHH
GFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRSGMIAFASSLDQAGVLTRTVKDA
HHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
ALMLQPICGYDTQDSTSANITTPRFLDSITNIIKGKRIGIPKEYELSNKYKEYEEVSEMW
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LKGIKYLENEGAEIVNISLPHTSYALPVYYIICSAEASSNLARYDGIKYGTRVNSDNINE
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCHHH
MYELTRGNNLGTEVKRRILIGAYALSSGYYDAYYNKAQCIRRLVTNDFVESFKSVDYILT
HHHHHCCCCCCHHHHHHHHHEEHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEC
PTAPKEAFAMDEQLDTLTMYLNDVFTVPASLAGLPAISIPIGLSKNKLPLSLQIIGNYYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCC
EGGILNIASVIEKYTGNILK
CCCEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVIYCHSDLFHSNMKDILKLSIAEMHENLIKKEFSAVELTQAHIDAINNEQLNAFITKTP
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
EVALNAARKADHILTHEQDKITSLTGIPVGIKDLFCTKNIKTTACSNILRNFTPQYDSTV
HHHHHHHHHHHHHHCCCCHHHHEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
VKRLIDDGAVMLGKLNMDEFAMGSSNSNSCFGPVENPWTRADGVKVVPGGSSGGSSAAVA
HHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHH
GFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRSGMIAFASSLDQAGVLTRTVKDA
HHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
ALMLQPICGYDTQDSTSANITTPRFLDSITNIIKGKRIGIPKEYELSNKYKEYEEVSEMW
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LKGIKYLENEGAEIVNISLPHTSYALPVYYIICSAEASSNLARYDGIKYGTRVNSDNINE
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCHHH
MYELTRGNNLGTEVKRRILIGAYALSSGYYDAYYNKAQCIRRLVTNDFVESFKSVDYILT
HHHHHCCCCCCHHHHHHHHHEEHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEC
PTAPKEAFAMDEQLDTLTMYLNDVFTVPASLAGLPAISIPIGLSKNKLPLSLQIIGNYYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCC
EGGILNIASVIEKYTGNILK
CCCEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA