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The map label for this gene is dagA [H]

Identifier: 58617042

GI number: 58617042

Start: 520166

End: 521551

Strand: Reverse

Name: dagA [H]

Synonym: ERGA_CDS_03150

Alternate gene names: 58617042

Gene position: 521551-520166 (Counterclockwise)

Preceding gene: 58617043

Following gene: 58617041

Centisome position: 34.77

GC content: 28.79

Gene sequence:

>1386_bases
ATGATAAACTCTATATTTACAGATTTTTTCCCTTATATATTAAATACGTTAGACTCTGTTCTCACTTTTACAATATTTAA
GATTCCTTTAATTATTTTATGGGTAATATGCCTTGGAATATTTTGTACTTTAAGATTTAAATTTATTAATATAAAACTCT
TTAAACATGCTTTATACATTTTAATTAATCCATCCAATAACAAATCTAATAATACAATTTCTAATATACAAGCATTTATT
ACTGCAATATCTGGAACTATAGGATTAGGTACAATTTCAGGAGTAGTAATCGCAGTTTCTACTGGAGGACCAGGTGCTGT
CTTATGGATGGTATTGATTGGAATATTAGGTATGTCAATAAAATTTGCTGAAGTCACATTAGGTTTCAAATACCGCATAT
TAACCGATAAATCCAGCATAGGAGGTCCTTTTCAATACATAAGATATAGTTTATCTGAATTAGGTTTAATTAAAACAGGA
AAAGCATTATCATATATATACGCCATCATTATGATAGTAGCTATGTTATTTGGAGGAATATCATTTCAATCAAATCAGGC
ATCTATTCTATTAAGTAATACTTTTAAACAAGTAAACAACAGCACTATTGTTCTAATACTAACTTTATTGAGTGGATTAG
TAATAGTAGGAGGGATAACACGCATTGCAAAGGTTGTGACAACATTATCTCCTATTATGGTCATATTATACATTACTATA
TGCATGTCAGTCATTATAAGACATATTGACCAGTTAATTCCTACATTTCAAACAATGTTCCATAGCATGATATATGGAAA
TAGCATCGCTGGAGGAGTAGTTGGCTCTTTAATAGCTGGAGTTAGAAGATCAGTTTTTACAAACGAAGCAGGTATTGGAA
CCTCCCCTATAGTACATTCATCTACACACGAAACTGAACCTGTAAGAGTAGGATGTATTGCTATGCTTGAGCCATTCATT
GACACAGTAATTGTATCATTTATTACAGGAATAGTAATAGTGTTAACTGGATCATATACACTAAGTAACAGTAATGATAT
CATTGAAATTAGCAATATATTCTCTAATACTTCTCCAATTTGTGAATACATAATATTTCCACTTATTGTCTTTGCTTTTG
CATTTTCTTCAATCATCACATATTCTTATTACTGTGAAATATCATGGAAATACCTGTTAAAAAGCAATTATAGTATCATA
TTATGCCGAATCTTAATTATTATTATTATCATCACTAGTGGATTATCAAAAAACATTATTTCTATATCACAATTAGGAGA
CTTACTTTTTATATTTCTCACCATACCTAATGGACTGACTATAATACTATTAAGTAAAGATATAAATAAAGAACTCAACT
TATACCTACAAAAAATAAAAACTTAA

Upstream 100 bases:

>100_bases
CATACTATCAATCCCTATAAGTAGTATGGTATACATATATCTTATGTACAAAAATAAAAATAAGAAAAACATATGATTAG
ATTAGCTATATCATAATCAT

Downstream 100 bases:

>100_bases
AATGATTATTTTACCTTATCTAAGTGGTTATAAAACAGATATATAAAAATACAATAAATCAGTTAGCATATTAGCTATTA
TAAGAAGATACTATTATTAT

Product: hypothetical protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MINSIFTDFFPYILNTLDSVLTFTIFKIPLIILWVICLGIFCTLRFKFINIKLFKHALYILINPSNNKSNNTISNIQAFI
TAISGTIGLGTISGVVIAVSTGGPGAVLWMVLIGILGMSIKFAEVTLGFKYRILTDKSSIGGPFQYIRYSLSELGLIKTG
KALSYIYAIIMIVAMLFGGISFQSNQASILLSNTFKQVNNSTIVLILTLLSGLVIVGGITRIAKVVTTLSPIMVILYITI
CMSVIIRHIDQLIPTFQTMFHSMIYGNSIAGGVVGSLIAGVRRSVFTNEAGIGTSPIVHSSTHETEPVRVGCIAMLEPFI
DTVIVSFITGIVIVLTGSYTLSNSNDIIEISNIFSNTSPICEYIIFPLIVFAFAFSSIITYSYYCEISWKYLLKSNYSII
LCRILIIIIIITSGLSKNIISISQLGDLLFIFLTIPNGLTIILLSKDINKELNLYLQKIKT

Sequences:

>Translated_461_residues
MINSIFTDFFPYILNTLDSVLTFTIFKIPLIILWVICLGIFCTLRFKFINIKLFKHALYILINPSNNKSNNTISNIQAFI
TAISGTIGLGTISGVVIAVSTGGPGAVLWMVLIGILGMSIKFAEVTLGFKYRILTDKSSIGGPFQYIRYSLSELGLIKTG
KALSYIYAIIMIVAMLFGGISFQSNQASILLSNTFKQVNNSTIVLILTLLSGLVIVGGITRIAKVVTTLSPIMVILYITI
CMSVIIRHIDQLIPTFQTMFHSMIYGNSIAGGVVGSLIAGVRRSVFTNEAGIGTSPIVHSSTHETEPVRVGCIAMLEPFI
DTVIVSFITGIVIVLTGSYTLSNSNDIIEISNIFSNTSPICEYIIFPLIVFAFAFSSIITYSYYCEISWKYLLKSNYSII
LCRILIIIIIITSGLSKNIISISQLGDLLFIFLTIPNGLTIILLSKDINKELNLYLQKIKT
>Mature_461_residues
MINSIFTDFFPYILNTLDSVLTFTIFKIPLIILWVICLGIFCTLRFKFINIKLFKHALYILINPSNNKSNNTISNIQAFI
TAISGTIGLGTISGVVIAVSTGGPGAVLWMVLIGILGMSIKFAEVTLGFKYRILTDKSSIGGPFQYIRYSLSELGLIKTG
KALSYIYAIIMIVAMLFGGISFQSNQASILLSNTFKQVNNSTIVLILTLLSGLVIVGGITRIAKVVTTLSPIMVILYITI
CMSVIIRHIDQLIPTFQTMFHSMIYGNSIAGGVVGSLIAGVRRSVFTNEAGIGTSPIVHSSTHETEPVRVGCIAMLEPFI
DTVIVSFITGIVIVLTGSYTLSNSNDIIEISNIFSNTSPICEYIIFPLIVFAFAFSSIITYSYYCEISWKYLLKSNYSII
LCRILIIIIIITSGLSKNIISISQLGDLLFIFLTIPNGLTIILLSKDINKELNLYLQKIKT

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=468, Percent_Identity=25.8547008547009, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50600; Mature: 50600

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSIFTDFFPYILNTLDSVLTFTIFKIPLIILWVICLGIFCTLRFKFINIKLFKHALYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
LINPSNNKSNNTISNIQAFITAISGTIGLGTISGVVIAVSTGGPGAVLWMVLIGILGMSI
EECCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCE
KFAEVTLGFKYRILTDKSSIGGPFQYIRYSLSELGLIKTGKALSYIYAIIMIVAMLFGGI
EEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SFQSNQASILLSNTFKQVNNSTIVLILTLLSGLVIVGGITRIAKVVTTLSPIMVILYITI
CCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CMSVIIRHIDQLIPTFQTMFHSMIYGNSIAGGVVGSLIAGVRRSVFTNEAGIGTSPIVHS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
STHETEPVRVGCIAMLEPFIDTVIVSFITGIVIVLTGSYTLSNSNDIIEISNIFSNTSPI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCEEEEHHHHCCCHHH
CEYIIFPLIVFAFAFSSIITYSYYCEISWKYLLKSNYSIILCRILIIIIIITSGLSKNII
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
SISQLGDLLFIFLTIPNGLTIILLSKDINKELNLYLQKIKT
HHHHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MINSIFTDFFPYILNTLDSVLTFTIFKIPLIILWVICLGIFCTLRFKFINIKLFKHALYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
LINPSNNKSNNTISNIQAFITAISGTIGLGTISGVVIAVSTGGPGAVLWMVLIGILGMSI
EECCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCE
KFAEVTLGFKYRILTDKSSIGGPFQYIRYSLSELGLIKTGKALSYIYAIIMIVAMLFGGI
EEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SFQSNQASILLSNTFKQVNNSTIVLILTLLSGLVIVGGITRIAKVVTTLSPIMVILYITI
CCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CMSVIIRHIDQLIPTFQTMFHSMIYGNSIAGGVVGSLIAGVRRSVFTNEAGIGTSPIVHS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
STHETEPVRVGCIAMLEPFIDTVIVSFITGIVIVLTGSYTLSNSNDIIEISNIFSNTSPI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCEEEEHHHHCCCHHH
CEYIIFPLIVFAFAFSSIITYSYYCEISWKYLLKSNYSIILCRILIIIIIITSGLSKNII
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
SISQLGDLLFIFLTIPNGLTIILLSKDINKELNLYLQKIKT
HHHHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]