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The map label for this gene is nuoB [H]

Identifier: 58617036

GI number: 58617036

Start: 511765

End: 512283

Strand: Reverse

Name: nuoB [H]

Synonym: ERGA_CDS_03090

Alternate gene names: 58617036

Gene position: 512283-511765 (Counterclockwise)

Preceding gene: 58617037

Following gene: 58617034

Centisome position: 34.15

GC content: 36.99

Gene sequence:

>519_bases
ATGATAAATAAGCATAATTCTGTTTTACATAATCAATTCTGGCAAAGTTATAATCATCGTGGATTCATGGTTACTAAACT
CTCAGATCTCATGAGCTATGTTTCAAGTTGGGCAAGATCAAATTCCTTATGGCCAATGACATTTGGATTAGCATGTTGTG
CAGTGGAAATGATGCACACAGCAGCAAGCAAATATGACTTAGATAGATATGGCATAATGTTTCGTGCAAGTCCAAGACAA
GCAGATGTCATGATTGTTGCAGGAACACTAACAAACAAAATGGCACCTGCACTACGTAAAGTATATGACCAAATGACAGA
ACCTCGTTATGTTATCTCAATGGGAAGTTGTGCTAATGGTGGAGGATATTACCACTACTCATATTCAGTAGTAAGAGGAT
GTGATCGCATTGTACCAGTAGATATCTATGTTCCAGGATGCCCACCTACAGCAGAAGCACTATTATATGGAATATTATGT
TTACAACAAAAAATCAATAGAACTGCTACTTCCACTTAA

Upstream 100 bases:

>100_bases
TGTAGGTGGATTTTGGTCTATGATGATTTTCTTAGTAATATTAATCATAGGATTCATATATGAATGGAGTAAAGGTGCAT
TAGAATGGGAGTAATTTTAT

Downstream 100 bases:

>100_bases
AAAAAGTACTTATTAATTAATAGTTAAACGTATACCTTTTTAAATAGAAAAAGCTATATCATAAAAATTTTTCATAAGTT
TACTAATCTTAAAATCTAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MINKHNSVLHNQFWQSYNHRGFMVTKLSDLMSYVSSWARSNSLWPMTFGLACCAVEMMHTAASKYDLDRYGIMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILC
LQQKINRTATST

Sequences:

>Translated_172_residues
MINKHNSVLHNQFWQSYNHRGFMVTKLSDLMSYVSSWARSNSLWPMTFGLACCAVEMMHTAASKYDLDRYGIMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILC
LQQKINRTATST
>Mature_172_residues
MINKHNSVLHNQFWQSYNHRGFMVTKLSDLMSYVSSWARSNSLWPMTFGLACCAVEMMHTAASKYDLDRYGIMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILC
LQQKINRTATST

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=82.1917808219178, Blast_Score=261, Evalue=3e-70,
Organism=Escherichia coli, GI1788624, Length=165, Percent_Identity=47.2727272727273, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=43.0894308943089, Blast_Score=104, Evalue=3e-24,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.8373983739837, Blast_Score=97, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=67.0967741935484, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=77.5510204081633, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=77.5510204081633, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.7671232876712, Blast_Score=250, Evalue=3e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19346; Mature: 19346

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKHNSVLHNQFWQSYNHRGFMVTKLSDLMSYVSSWARSNSLWPMTFGLACCAVEMMHT
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASKYDLDRYGIMFRASPRQADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANG
HHHHCCCHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILCLQQKINRTATST
CCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINKHNSVLHNQFWQSYNHRGFMVTKLSDLMSYVSSWARSNSLWPMTFGLACCAVEMMHT
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASKYDLDRYGIMFRASPRQADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANG
HHHHCCCHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILCLQQKINRTATST
CCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA