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The map label for this gene is miaB [H]

Identifier: 58616989

GI number: 58616989

Start: 427460

End: 428812

Strand: Reverse

Name: miaB [H]

Synonym: ERGA_CDS_02620

Alternate gene names: 58616989

Gene position: 428812-427460 (Counterclockwise)

Preceding gene: 58616990

Following gene: 58616985

Centisome position: 28.59

GC content: 31.71

Gene sequence:

>1353_bases
TTGTTTGTAAAACTAATGCAAGGTCTATATATAAAGTCATATGGCTGTCAGATGAACGTTTACGACTCTCTGATTATAGA
AAATATTATTAAGCCTCTTGGCTTTAGTATAGTAAATGAACTATCAGAAGCAGATATTGTAATATTAAACACTTGTCATA
TAAGAGAGAAAGCAGCAGAAAAGTTGTATTCAGAATTAGGCAGGATAAGAAAAATACAAGAGACCAAAAACTTAACCATA
GTAGTTGCTGGATGTGTTGCACAAGCAGAAGGAACAGAAATATTCACCAGAGCACCATTCGTAGATATAGTAGTAGGCCC
TCAAAGTATACATACACTACCTGAACTAATCGTCAAAGCGCGTAAAATAAAAAAGCAGATCATTAATATCGATTTTCCTG
TAATCTCAAAATTTGATGCGATTGCAGTAGAAGAATATACAAAAAATCAAAAAGTTTCTGCTTTCATTTCAGTACAAGAA
GGATGTAATAAATTTTGTTCATTCTGCGTAGTTCCATATACTAGAGGAGAAGAGTATTCAAGAACAGTAGAGGCAATTTT
TAAAGAAGCACTTATTTTAGCAGATTCCGGAATAAAAGAAATTACACTAATTGGACAGAACGTAAATGCATACCATGGAA
CATACAAAGGTAATGAGTGGGATTTAGGTAGATTAATTCAACACATCGCAAAAATATCAAGTATAGAACGTATATATTAT
ACAACATCACACCCACGTGATATGCATGAATCCTTATATGAAGCACATGGAATTGAAAAAAAGTTAATACCATTCATTCA
TTTACCAGTACAATCAGGTTCAAATAAAATATTACGAAAAATGAATAGAAAGCACACTGCTGAAGAATACATAAATATAA
TCAAAACTCTACGTAAACACCGTAGTGATATTGCTTATTCATCTGATTTCATAGTTGGATTTCCAGGAGAAACAGATGAA
GATTTTGAGAATACAATACGGTTGATAGAAGAAGTAAAGTTTTCACAGGCATATAGTTTTAAGTATAGTCCAAGACCAGG
AACACCAAGTGCAGAATATACAAGTCAGATACCAGATGAAATAAAATCACAGCGTCTCACTAAATTACAAGAATTAGTAC
ACAAACAACAACTTGAATTTAATAAGAAAATGATTGGTGAAACTCATCCTGTACTTTTTTATAAGAAAGGAAAGTTTGAT
AACCAAATTATTGGGAAAACTCCATATATGCAGTCATGCTATATTAATACAGAAAATCCAGATTTATATTACAATAAAAT
AGTTCCTATTAAAATAACAGACGCTCATAAGAACCATTTAACTGGTATAATACCTCACACACTTCCTGTTTAG

Upstream 100 bases:

>100_bases
TATACATAATTTCTCGTAATTATAAAATATTCAGGAAGTTAACATAACAAAAACTATTGTTTTGATTTATTAATCATTAT
ATGCTTATAAACTAAAATGG

Downstream 100 bases:

>100_bases
GAGAACTTAATTCATTATAAATATCGGTAAAATACTAATATGGATATAACATTATAACTAATAACGTATTACACAATTAA
GAAAATTTCGTCTTGAAATG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MFVKLMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAEKLYSELGRIRKIQETKNLTI
VVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKKQIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQE
GCNKFCSFCVVPYTRGEEYSRTVEAIFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYY
TTSHPRDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIAYSSDFIVGFPGETDE
DFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQRLTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFD
NQIIGKTPYMQSCYINTENPDLYYNKIVPIKITDAHKNHLTGIIPHTLPV

Sequences:

>Translated_450_residues
MFVKLMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAEKLYSELGRIRKIQETKNLTI
VVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKKQIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQE
GCNKFCSFCVVPYTRGEEYSRTVEAIFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYY
TTSHPRDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIAYSSDFIVGFPGETDE
DFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQRLTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFD
NQIIGKTPYMQSCYINTENPDLYYNKIVPIKITDAHKNHLTGIIPHTLPV
>Mature_450_residues
MFVKLMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAEKLYSELGRIRKIQETKNLTI
VVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKKQIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQE
GCNKFCSFCVVPYTRGEEYSRTVEAIFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYY
TTSHPRDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIAYSSDFIVGFPGETDE
DFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQRLTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFD
NQIIGKTPYMQSCYINTENPDLYYNKIVPIKITDAHKNHLTGIIPHTLPV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=413, Percent_Identity=32.2033898305085, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI28872782, Length=413, Percent_Identity=32.2033898305085, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=26.4770240700219, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=43.4004474272931, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=26.6365688487585, Blast_Score=168, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=417, Percent_Identity=32.8537170263789, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=317, Percent_Identity=26.4984227129338, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356207, Length=419, Percent_Identity=29.8329355608592, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI19922432, Length=396, Percent_Identity=26.5151515151515, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51472; Mature: 51472

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVKLMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAE
CHHHHHHCHHEEECCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHH
KLYSELGRIRKIQETKNLTIVVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKA
HHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHHHH
RKIKKQIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQEGCNKFCSFCVVPYTRGEEYS
HHHHHHHHCCCCHHHCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHHEEEEECCCCCHHH
RTVEAIFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYY
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
TTSHPRDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKH
ECCCCHHHHHHHHHHHCCCHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RSDIAYSSDFIVGFPGETDEDFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDE
HHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCHHH
IKSQRLTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFDNQIIGKTPYMQSCYINTENP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
DLYYNKIVPIKITDAHKNHLTGIIPHTLPV
CEEEEEEEEEEEECCCCCCCCEECCCCCCC
>Mature Secondary Structure
MFVKLMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAE
CHHHHHHCHHEEECCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHH
KLYSELGRIRKIQETKNLTIVVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKA
HHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHHHH
RKIKKQIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQEGCNKFCSFCVVPYTRGEEYS
HHHHHHHHCCCCHHHCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHHEEEEECCCCCHHH
RTVEAIFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYY
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
TTSHPRDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKH
ECCCCHHHHHHHHHHHCCCHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RSDIAYSSDFIVGFPGETDEDFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDE
HHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCHHH
IKSQRLTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFDNQIIGKTPYMQSCYINTENP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
DLYYNKIVPIKITDAHKNHLTGIIPHTLPV
CEEEEEEEEEEEECCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA