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The map label for this gene is puuA [H]

Identifier: 58616867

GI number: 58616867

Start: 232606

End: 233412

Strand: Reverse

Name: puuA [H]

Synonym: ERGA_CDS_01400

Alternate gene names: 58616867

Gene position: 233412-232606 (Counterclockwise)

Preceding gene: 58616868

Following gene: 58616861

Centisome position: 15.56

GC content: 27.14

Gene sequence:

>807_bases
ATGTTAGAATATACACTAAATTATCTTACTAATAATCTAAATATAACTCCAGTAATAGGCATAGAACTAGAGTTTTACTT
TGACCATATATCATTAGACAATTTGAAATTAGTTATTGATAACATAAAAAATAAACTATCTTGTTTTAACTGTAATATAA
TACAAGAAAAAAGCGAATTACAATATGAAATACAAACATCATATACCACTAATATTTCTAGACTTATATCTGAAATTAAT
ACAATTAAAGAAATTTTGCATAATACAGTACAAAACCATAAAGGAACCATTAATTTTTCTGCAAAACCATATTTAGATAA
ACCAGGGAGTGCATTTCATGTGCACATAAACTTACTTGATAAAAATGCAAACAATCTATTTTTCCAACAAAATAACGAAG
TAAGTACATATTTGTTATACAGCATAGGAGGCTTATGTACATTAATGAAAAAACATATGATATTTTTTGCACCATATAGT
GATTCATATTTAAGATATATACATGCTGATATAGATACTCCTACAACTATCAGCTGGGGAGGAAATAATAGAAGCACTGC
TATAAGGATACCAAACACTACAAACAATCCTAATAGATGTAGAATAGAACACAGAGTAGCTGGTTCAGATTGCAATCATA
AACAAGTAATCACTTCTATTTTACAGGGATTAATTCATGGAATACAAAACAAAATACAACCTATTCCTAGAACTTATGGA
ATTGCATCAGATTTACAATATAACCTAGAAAAATTACCATTAAGTTTAGACGAATCAATAAAACACTTCATTACAGACCA
ACAATAA

Upstream 100 bases:

>100_bases
CTTTAGGACTTACCCTATTCATAATTACATGGTTTCTCAATATACTTGCACTACATATCACAAAAAAGACAGTAAAACCA
ATTAAAGTTAATTAGTACAT

Downstream 100 bases:

>100_bases
GAACTTTTAAGCTACTATTATCCATAAAATAATATAGCCATCATCATATTCTTATTACTTTCTATTAAATACCCTAATAA
ACCAACACAAGGATTTTTTT

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLEYTLNYLTNNLNITPVIGIELEFYFDHISLDNLKLVIDNIKNKLSCFNCNIIQEKSELQYEIQTSYTTNISRLISEIN
TIKEILHNTVQNHKGTINFSAKPYLDKPGSAFHVHINLLDKNANNLFFQQNNEVSTYLLYSIGGLCTLMKKHMIFFAPYS
DSYLRYIHADIDTPTTISWGGNNRSTAIRIPNTTNNPNRCRIEHRVAGSDCNHKQVITSILQGLIHGIQNKIQPIPRTYG
IASDLQYNLEKLPLSLDESIKHFITDQQ

Sequences:

>Translated_268_residues
MLEYTLNYLTNNLNITPVIGIELEFYFDHISLDNLKLVIDNIKNKLSCFNCNIIQEKSELQYEIQTSYTTNISRLISEIN
TIKEILHNTVQNHKGTINFSAKPYLDKPGSAFHVHINLLDKNANNLFFQQNNEVSTYLLYSIGGLCTLMKKHMIFFAPYS
DSYLRYIHADIDTPTTISWGGNNRSTAIRIPNTTNNPNRCRIEHRVAGSDCNHKQVITSILQGLIHGIQNKIQPIPRTYG
IASDLQYNLEKLPLSLDESIKHFITDQQ
>Mature_268_residues
MLEYTLNYLTNNLNITPVIGIELEFYFDHISLDNLKLVIDNIKNKLSCFNCNIIQEKSELQYEIQTSYTTNISRLISEIN
TIKEILHNTVQNHKGTINFSAKPYLDKPGSAFHVHINLLDKNANNLFFQQNNEVSTYLLYSIGGLCTLMKKHMIFFAPYS
DSYLRYIHADIDTPTTISWGGNNRSTAIRIPNTTNNPNRCRIEHRVAGSDCNHKQVITSILQGLIHGIQNKIQPIPRTYG
IASDLQYNLEKLPLSLDESIKHFITDQQ

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=263, Percent_Identity=29.277566539924, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790301, Length=213, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 30733; Mature: 30733

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYTLNYLTNNLNITPVIGIELEFYFDHISLDNLKLVIDNIKNKLSCFNCNIIQEKSEL
CCEEEEEHHHCCCEEEEEEEEEEEEEEEEECCCCHHHEEHHHHCHHEEEECEEEECCCCC
QYEIQTSYTTNISRLISEINTIKEILHNTVQNHKGTINFSAKPYLDKPGSAFHVHINLLD
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEE
KNANNLFFQQNNEVSTYLLYSIGGLCTLMKKHMIFFAPYSDSYLRYIHADIDTPTTISWG
CCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEEC
GNNRSTAIRIPNTTNNPNRCRIEHRVAGSDCNHKQVITSILQGLIHGIQNKIQPIPRTYG
CCCCCEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCC
IASDLQYNLEKLPLSLDESIKHFITDQQ
CHHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLEYTLNYLTNNLNITPVIGIELEFYFDHISLDNLKLVIDNIKNKLSCFNCNIIQEKSEL
CCEEEEEHHHCCCEEEEEEEEEEEEEEEEECCCCHHHEEHHHHCHHEEEECEEEECCCCC
QYEIQTSYTTNISRLISEINTIKEILHNTVQNHKGTINFSAKPYLDKPGSAFHVHINLLD
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEE
KNANNLFFQQNNEVSTYLLYSIGGLCTLMKKHMIFFAPYSDSYLRYIHADIDTPTTISWG
CCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEEC
GNNRSTAIRIPNTTNNPNRCRIEHRVAGSDCNHKQVITSILQGLIHGIQNKIQPIPRTYG
CCCCCEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCC
IASDLQYNLEKLPLSLDESIKHFITDQQ
CHHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]