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The map label for this gene is polA [H]

Identifier: 58616765

GI number: 58616765

Start: 49747

End: 52329

Strand: Reverse

Name: polA [H]

Synonym: ERGA_CDS_00380

Alternate gene names: 58616765

Gene position: 52329-49747 (Counterclockwise)

Preceding gene: 58616770

Following gene: 58616764

Centisome position: 3.49

GC content: 30.39

Gene sequence:

>2583_bases
ATGAGTACTTTTACAATTATAGATGCTTATGGTCTACTTTTTAGAGCATATTACGCTTTACCTAATCTTAGTACATCTAA
TGGTGTACCTATTGGTGGAGTATATGGCTTTATTAATATGTTACTGAAATATATAGAAGTTCATACTGCAAATTACCTAG
TTGTCGTATTTGACACTGGTGGTAAAAATTTTCGTCATGATATATACCCACAATATAAAAGCAATCGTATTAAGCTCCCT
GAAGATTTGATATCTCAATTTCCACTACTTAGAGAAGCTGTTAATGCACTTAATATATCATATGAAGAAGTTGCTGGATA
TGAAGCTGATGACGTAATAGCAACTTTGAGTAAAAACTACAGCAAATTTGATGATATAAAAGTCACAGTTGTTACATCAG
ATAAAGACTTGCTGCAGCTTTTAGAACATAATATTCATATTTTCGATCCTGTCAAAAATAAATACATCGTAGAAGATGAC
GTACAAGATAAGTTTGGTATATCATCAAGTAAATTGCTGGACTTCTTATCTCTGACTGGTGACACATCAGATAATATTCC
AGGTGTACCTAGTATAGGTATCAAAACCGCTGCAAAATTATTGAACAATTTCGGATCACTAGATAATCTACTATCACAAC
CACATGCCATTGAAAAAAATAAGTGTCGCGAATCAATTATTAAATATCAAGATCAGGCAATATTATCTCGTCAGCTTTTA
ACATTATGTGATACAGTAAACTTAGATAATAACATAGAAAAGTACATATCTCGTATTCCTGATACGCAAAAGCTAGTGAA
ATTCTTAAAAAAGTACGAACTTCAATCCTTAATTAATAAAATTGGTAAGATTTTTAAAACAGATGCTTTTACTGTATATC
AAAAATCGGGATATAATAATGAGAATAGTATAAAAACTAACGTAATACCATATTCCTCTGAGGAACTCAAAACTTTTATT
GAGAATTGTAAAAACGAAGGAATAATAGCCTTATATATTGATATAACTGATAACACAATCAATAGTATGTCTTTATCCTA
TAAGGAAGGTACTATACTCTACATTAATAAGGAACATATAATTCACGCTTTGGATTTAATTAAGCCACTCTTCAGTTTAG
ATCACATACTAAAAGTGATGTACGATGTAAAAACTCTATTCAAAATTATTCAAGAAGACACCAATATAACAGCTTTTGAT
GATATATTAATAATGTCATATAGCCTAGATGTAGGATTACATGACCATACATTAAAAAACATCATTATACGTAACACTGA
CAAACATGATATTACTAATGAAAACATCACAGCATCTACTTTATTATCATTACACACAGCATTAAGAAGAAATTTATTTA
CAAACCAGTTGTGTACTATTTATTACAGGATAGAAAAACCACTAATTAATGTTTTACATAATATGGAAAAAATAGGAATA
ATGATTGATGGTAGTATTCTAGATAAGCTATCATCCACTTTTTCAGATAAGATTAGTGTGTTAGAAGAAAAAATATATAA
ATTGTCAGGAGAAGAATTCAACATAGCTTCTTCAAGACAACTAAGCACCATATTGTTTGACAAAATGGGTATAAAAAAAG
GTAAAAAGCTAAGTTCAGGTACTTATAGTACAGATGCAGAAGTGTTACACGAACTTGCATTTAATGGGGTTGAAATAGCT
GATGAGATACTAAAATGGCGTCACTTTACAAAACTGAAAAACACTTATACTGATGTCTTGGCAAAACAAATAGATAATAA
TACCAGCAGAGTACATACGTTTTACTCGATGACATCCACTGTAACTGGTAGGTTAAGTTCAAGTAATCCTAACCTGCAAA
ATATTCCTATTAGAAGTGAAGAAGGAAATGCTATTAGAAATGCATTTATAGCAAAAAGTGGATATAAATTAATTTCCGCT
GATTATTCACAAATAGAGTTACGTATAATGGCACATATTGCTAGTGTACAAGCATTCAGAGATGCATTTTCTTTGGACCA
AGATATACATTTAATTACAGCACAACAGATCTTTAATACACAAAATATAGATAAGAAATTACGTAGAAAAGCAAAATCAA
TTAATTTTGGAATAATCTATGGTATCAGTCCATTTGGATTAGCTAAACAACTTGGTATCACACGATCAGAAGCAAGTAAA
CACATCGAAAACTATTTTAAGTCTTATCCAGAGATAAAATCCTATATAGAAGACATAAAAGTTTACGCAAGAACTCATGG
GTATACAAGGACTGTATTTGGTAGAAAGTGTTTTATAAAAGATATTAATAGTAATAACCACGCATTAAGAAATTTTTCTG
AAAGAGCAGCAGTTAATGCTCCACTCCAAGGAACATCCGCTGACATAATCAAAATGTCAATGATACACCTATTCGATAGA
TTAAAAACTGGATATATGATATTACAAGTACATGACGAGCTATTATTTGAAGTACCAGAAGATCACGTTGATAATACAGT
AAAACTCATCAAAGAAGTTATGGAAGGTATTGTAAAATTATCAGTACCACTCAAGGTAGATATAACTATAGGTGACAACT
GGAGCAATTTAGTACCATATTAA

Upstream 100 bases:

>100_bases
TATTGAATATATTCTATAGTTTTTTATATATGCAGTGTACTAATAATATGTAAAAGTGTACAATCTACTAGGATAAGATT
ATTTACTGCATTTAACATTA

Downstream 100 bases:

>100_bases
TACACATAACTTAAGAACTCCATTGGATATATGATATTACAAAGTATATGGCAAGCTACTAATTTGAAGTACCAGAAGAT
CACGTTGATAATACAGTAAA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 860; Mature: 859

Protein sequence:

>860_residues
MSTFTIIDAYGLLFRAYYALPNLSTSNGVPIGGVYGFINMLLKYIEVHTANYLVVVFDTGGKNFRHDIYPQYKSNRIKLP
EDLISQFPLLREAVNALNISYEEVAGYEADDVIATLSKNYSKFDDIKVTVVTSDKDLLQLLEHNIHIFDPVKNKYIVEDD
VQDKFGISSSKLLDFLSLTGDTSDNIPGVPSIGIKTAAKLLNNFGSLDNLLSQPHAIEKNKCRESIIKYQDQAILSRQLL
TLCDTVNLDNNIEKYISRIPDTQKLVKFLKKYELQSLINKIGKIFKTDAFTVYQKSGYNNENSIKTNVIPYSSEELKTFI
ENCKNEGIIALYIDITDNTINSMSLSYKEGTILYINKEHIIHALDLIKPLFSLDHILKVMYDVKTLFKIIQEDTNITAFD
DILIMSYSLDVGLHDHTLKNIIIRNTDKHDITNENITASTLLSLHTALRRNLFTNQLCTIYYRIEKPLINVLHNMEKIGI
MIDGSILDKLSSTFSDKISVLEEKIYKLSGEEFNIASSRQLSTILFDKMGIKKGKKLSSGTYSTDAEVLHELAFNGVEIA
DEILKWRHFTKLKNTYTDVLAKQIDNNTSRVHTFYSMTSTVTGRLSSSNPNLQNIPIRSEEGNAIRNAFIAKSGYKLISA
DYSQIELRIMAHIASVQAFRDAFSLDQDIHLITAQQIFNTQNIDKKLRRKAKSINFGIIYGISPFGLAKQLGITRSEASK
HIENYFKSYPEIKSYIEDIKVYARTHGYTRTVFGRKCFIKDINSNNHALRNFSERAAVNAPLQGTSADIIKMSMIHLFDR
LKTGYMILQVHDELLFEVPEDHVDNTVKLIKEVMEGIVKLSVPLKVDITIGDNWSNLVPY

Sequences:

>Translated_860_residues
MSTFTIIDAYGLLFRAYYALPNLSTSNGVPIGGVYGFINMLLKYIEVHTANYLVVVFDTGGKNFRHDIYPQYKSNRIKLP
EDLISQFPLLREAVNALNISYEEVAGYEADDVIATLSKNYSKFDDIKVTVVTSDKDLLQLLEHNIHIFDPVKNKYIVEDD
VQDKFGISSSKLLDFLSLTGDTSDNIPGVPSIGIKTAAKLLNNFGSLDNLLSQPHAIEKNKCRESIIKYQDQAILSRQLL
TLCDTVNLDNNIEKYISRIPDTQKLVKFLKKYELQSLINKIGKIFKTDAFTVYQKSGYNNENSIKTNVIPYSSEELKTFI
ENCKNEGIIALYIDITDNTINSMSLSYKEGTILYINKEHIIHALDLIKPLFSLDHILKVMYDVKTLFKIIQEDTNITAFD
DILIMSYSLDVGLHDHTLKNIIIRNTDKHDITNENITASTLLSLHTALRRNLFTNQLCTIYYRIEKPLINVLHNMEKIGI
MIDGSILDKLSSTFSDKISVLEEKIYKLSGEEFNIASSRQLSTILFDKMGIKKGKKLSSGTYSTDAEVLHELAFNGVEIA
DEILKWRHFTKLKNTYTDVLAKQIDNNTSRVHTFYSMTSTVTGRLSSSNPNLQNIPIRSEEGNAIRNAFIAKSGYKLISA
DYSQIELRIMAHIASVQAFRDAFSLDQDIHLITAQQIFNTQNIDKKLRRKAKSINFGIIYGISPFGLAKQLGITRSEASK
HIENYFKSYPEIKSYIEDIKVYARTHGYTRTVFGRKCFIKDINSNNHALRNFSERAAVNAPLQGTSADIIKMSMIHLFDR
LKTGYMILQVHDELLFEVPEDHVDNTVKLIKEVMEGIVKLSVPLKVDITIGDNWSNLVPY
>Mature_859_residues
STFTIIDAYGLLFRAYYALPNLSTSNGVPIGGVYGFINMLLKYIEVHTANYLVVVFDTGGKNFRHDIYPQYKSNRIKLPE
DLISQFPLLREAVNALNISYEEVAGYEADDVIATLSKNYSKFDDIKVTVVTSDKDLLQLLEHNIHIFDPVKNKYIVEDDV
QDKFGISSSKLLDFLSLTGDTSDNIPGVPSIGIKTAAKLLNNFGSLDNLLSQPHAIEKNKCRESIIKYQDQAILSRQLLT
LCDTVNLDNNIEKYISRIPDTQKLVKFLKKYELQSLINKIGKIFKTDAFTVYQKSGYNNENSIKTNVIPYSSEELKTFIE
NCKNEGIIALYIDITDNTINSMSLSYKEGTILYINKEHIIHALDLIKPLFSLDHILKVMYDVKTLFKIIQEDTNITAFDD
ILIMSYSLDVGLHDHTLKNIIIRNTDKHDITNENITASTLLSLHTALRRNLFTNQLCTIYYRIEKPLINVLHNMEKIGIM
IDGSILDKLSSTFSDKISVLEEKIYKLSGEEFNIASSRQLSTILFDKMGIKKGKKLSSGTYSTDAEVLHELAFNGVEIAD
EILKWRHFTKLKNTYTDVLAKQIDNNTSRVHTFYSMTSTVTGRLSSSNPNLQNIPIRSEEGNAIRNAFIAKSGYKLISAD
YSQIELRIMAHIASVQAFRDAFSLDQDIHLITAQQIFNTQNIDKKLRRKAKSINFGIIYGISPFGLAKQLGITRSEASKH
IENYFKSYPEIKSYIEDIKVYARTHGYTRTVFGRKCFIKDINSNNHALRNFSERAAVNAPLQGTSADIIKMSMIHLFDRL
KTGYMILQVHDELLFEVPEDHVDNTVKLIKEVMEGIVKLSVPLKVDITIGDNWSNLVPY

Specific function: In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE1 insert domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=469, Percent_Identity=28.1449893390192, Blast_Score=198, Evalue=2e-50,
Organism=Homo sapiens, GI139394648, Length=519, Percent_Identity=25.4335260115607, Blast_Score=168, Evalue=2e-41,
Organism=Escherichia coli, GI1790294, Length=917, Percent_Identity=37.73173391494, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI226510970, Length=205, Percent_Identity=30.2439024390244, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI86563272, Length=447, Percent_Identity=28.4116331096197, Blast_Score=173, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17933644, Length=424, Percent_Identity=30.4245283018868, Blast_Score=187, Evalue=3e-47,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR005728
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 97693; Mature: 97562

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFTIIDAYGLLFRAYYALPNLSTSNGVPIGGVYGFINMLLKYIEVHTANYLVVVFDTG
CCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GKNFRHDIYPQYKSNRIKLPEDLISQFPLLREAVNALNISYEEVAGYEADDVIATLSKNY
CCCCCCCCCCCCCCCCEECCHHHHHHCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCC
SKFDDIKVTVVTSDKDLLQLLEHNIHIFDPVKNKYIVEDDVQDKFGISSSKLLDFLSLTG
CCCCCEEEEEEECCHHHHHHHHCCCEEECCCCCCEEECCCCHHHHCCCHHHHHHHHHHCC
DTSDNIPGVPSIGIKTAAKLLNNFGSLDNLLSQPHAIEKNKCRESIIKYQDQAILSRQLL
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLCDTVNLDNNIEKYISRIPDTQKLVKFLKKYELQSLINKIGKIFKTDAFTVYQKSGYNN
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
ENSIKTNVIPYSSEELKTFIENCKNEGIIALYIDITDNTINSMSLSYKEGTILYINKEHI
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCCEEEEEEHHHH
IHALDLIKPLFSLDHILKVMYDVKTLFKIIQEDTNITAFDDILIMSYSLDVGLHDHTLKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEEEEEEECCCCCHHHHHH
IIIRNTDKHDITNENITASTLLSLHTALRRNLFTNQLCTIYYRIEKPLINVLHNMEKIGI
HEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCHHHHEE
MIDGSILDKLSSTFSDKISVLEEKIYKLSGEEFNIASSRQLSTILFDKMGIKKGKKLSSG
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
TYSTDAEVLHELAFNGVEIADEILKWRHFTKLKNTYTDVLAKQIDNNTSRVHTFYSMTST
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHHH
VTGRLSSSNPNLQNIPIRSEEGNAIRNAFIAKSGYKLISADYSQIELRIMAHIASVQAFR
HEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
DAFSLDQDIHLITAQQIFNTQNIDKKLRRKAKSINFGIIYGISPFGLAKQLGITRSEASK
HHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHH
HIENYFKSYPEIKSYIEDIKVYARTHGYTRTVFGRKCFIKDINSNNHALRNFSERAAVNA
HHHHHHHHCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHCCC
PLQGTSADIIKMSMIHLFDRLKTGYMILQVHDELLFEVPEDHVDNTVKLIKEVMEGIVKL
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
SVPLKVDITIGDNWSNLVPY
ECCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
STFTIIDAYGLLFRAYYALPNLSTSNGVPIGGVYGFINMLLKYIEVHTANYLVVVFDTG
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GKNFRHDIYPQYKSNRIKLPEDLISQFPLLREAVNALNISYEEVAGYEADDVIATLSKNY
CCCCCCCCCCCCCCCCEECCHHHHHHCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCC
SKFDDIKVTVVTSDKDLLQLLEHNIHIFDPVKNKYIVEDDVQDKFGISSSKLLDFLSLTG
CCCCCEEEEEEECCHHHHHHHHCCCEEECCCCCCEEECCCCHHHHCCCHHHHHHHHHHCC
DTSDNIPGVPSIGIKTAAKLLNNFGSLDNLLSQPHAIEKNKCRESIIKYQDQAILSRQLL
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLCDTVNLDNNIEKYISRIPDTQKLVKFLKKYELQSLINKIGKIFKTDAFTVYQKSGYNN
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
ENSIKTNVIPYSSEELKTFIENCKNEGIIALYIDITDNTINSMSLSYKEGTILYINKEHI
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCCEEEEEEHHHH
IHALDLIKPLFSLDHILKVMYDVKTLFKIIQEDTNITAFDDILIMSYSLDVGLHDHTLKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEEEEEEECCCCCHHHHHH
IIIRNTDKHDITNENITASTLLSLHTALRRNLFTNQLCTIYYRIEKPLINVLHNMEKIGI
HEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCHHHHEE
MIDGSILDKLSSTFSDKISVLEEKIYKLSGEEFNIASSRQLSTILFDKMGIKKGKKLSSG
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
TYSTDAEVLHELAFNGVEIADEILKWRHFTKLKNTYTDVLAKQIDNNTSRVHTFYSMTST
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHHH
VTGRLSSSNPNLQNIPIRSEEGNAIRNAFIAKSGYKLISADYSQIELRIMAHIASVQAFR
HEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
DAFSLDQDIHLITAQQIFNTQNIDKKLRRKAKSINFGIIYGISPFGLAKQLGITRSEASK
HHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHHH
HIENYFKSYPEIKSYIEDIKVYARTHGYTRTVFGRKCFIKDINSNNHALRNFSERAAVNA
HHHHHHHHCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHCCC
PLQGTSADIIKMSMIHLFDRLKTGYMILQVHDELLFEVPEDHVDNTVKLIKEVMEGIVKL
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
SVPLKVDITIGDNWSNLVPY
ECCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10486973; 11030655 [H]