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The map label for this gene is rihA [H]

Identifier: 57652502

GI number: 57652502

Start: 265414

End: 266349

Strand: Direct

Name: rihA [H]

Synonym: SACOL0225

Alternate gene names: 57652502

Gene position: 265414-266349 (Clockwise)

Preceding gene: 57652499

Following gene: 57652503

Centisome position: 9.45

GC content: 39.64

Gene sequence:

>936_bases
ATGAAAAGAAAGATTATTATGGATTGTGATCCAGGACACGATGATGCAATAGCATTAATTTTAGCGGGGGCAATTGACAG
TCCACTAGAGATATTAGCTGTAACAACAGTCGCAGGTAATCAATCAGTTGACAAGAATACGACAAACGCCTTGAACGTAT
TGGATATTATGGGACGCCAAGATATAGCAGTAGCGAAAGGTGCGGATAGGCCGTTAATTAAACCAGCTGCCTTTGCTTCT
GAAATACATGGGGAATCTGGATTAGATGGTCCGAAACTACCGTCGACACCATCACGTCAAGCAGTTGCAATGCCAGCATC
AGATGTGATTATAAACAAAGTGATGACGAGTGATACACCTGTAACAATTGTAGCGACAGGTCCTCTTACGAATGTAGCAA
CGGCATTGATTCGTGAGCCAAGAATCGCTGAGCATATTGAATCTATTACTTTGATGGGTGGTGGTACATTTGGAAATTGG
ACGCCTACAGCAGAATTCAATATTTGGGTAGATGCTGAAGCAGCGAAGCGTGTTTTTGAAAGTGGGATTACTATAAATGT
GTTTGGTTTAGATGTAACACATCAAGTTTTAGCCGACGATCACGTGATTGAACGCTTTGAAAGTATCAATAATCCTGTTG
CACAGTTCGTCGTAGAATTATTGCAATTCTTTAAGAAGACATACAAGACTCACTTTAATATGGATGGTGGTCCAATACAT
GATGCTTGTACAATTTTGTATTTGTTACAACCAGAATTGTTTACAATGGTACCCGTTAATATCGACATTGAACATCAAAG
TCCACTAACTTATGGCACTATGGCTGTCGATTTAAATCATGTTACAGGTAAGCCTGCCAATGCTTATTTTGCTACAGCAG
TTGATGTTGAAGAAGTGTGGAACTTGATAGACCATAAGTTACGTACATACGAATAA

Upstream 100 bases:

>100_bases
TATGAGAAAGTTAAAACTATTTTTCAAAATAAACATGATTCACCACATAAATAAATATACATAAGCTATCGATAACAACT
TAAGAAAGGTGGATATATAA

Downstream 100 bases:

>100_bases
TAATACTTAATTAAATAGATACAGTTAACCCTAAGGCGCCTGATATAAGCGTCCTTAGGGTTTTTGTATTGGGTTACAAT
GGTCATGACAATTTGATAAT

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVDKNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE

Sequences:

>Translated_311_residues
MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVDKNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE
>Mature_311_residues
MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVDKNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=313, Percent_Identity=45.3674121405751, Blast_Score=250, Evalue=7e-68,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=40.5144694533762, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1786213, Length=298, Percent_Identity=38.5906040268456, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=31.7460317460317, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=261, Percent_Identity=36.0153256704981, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24583915, Length=195, Percent_Identity=31.2820512820513, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641839, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33795; Mature: 33795

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00018 EF_HAND_1 ; PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVDKNTTNALNVLDIMGRQ
CCCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCC
DIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTP
CEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCC
VTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNWTPTAEFNIWVDAEAAKRVFE
EEEEECCCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHH
SGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVW
HHHHEEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEECCHHHHH
NLIDHKLRTYE
HHHHHHHHCCC
>Mature Secondary Structure
MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVDKNTTNALNVLDIMGRQ
CCCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCC
DIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTP
CEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCC
VTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNWTPTAEFNIWVDAEAAKRVFE
EEEEECCCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHH
SGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVW
HHHHEEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEECCHHHHH
NLIDHKLRTYE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA