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The map label for this gene is ldh2 [H]

Identifier: 57652337

GI number: 57652337

Start: 2674414

End: 2675373

Strand: Reverse

Name: ldh2 [H]

Synonym: SACOL2618

Alternate gene names: 57652337

Gene position: 2675373-2674414 (Counterclockwise)

Preceding gene: 57652339

Following gene: 57652336

Centisome position: 95.23

GC content: 35.83

Gene sequence:

>960_bases
ATGAAAACATTTGGTAAAAAGGTTGTATTAATCGGAGATGGATCTGTAGGATCAAGCTATGCCTTTGCAATGGTTACGCA
AGGTGTTGCTGATGAATTTGTAATTATTGACATTGCAAAAGACAAAGTAAAAGCAGATGTTCAAGATTTAAACCATGGTA
CAGTCCACAGTCCTTCACCAGTTGATGTGAAAGCAGGTGAATACGAAGACTGTAAAGATGCAGATTTAGTTGTTATTACA
GCTGGCGCACCTCAAAAGCCAGGTGAAACACGTTTACAATTAGTTGAAAAAAATACTAAGATTATGAAGAGCATCGTTAA
GAGTGTTATGGATAGTGGCTTTGATGGATATTTCTTAATCGCGGCAAACCCTGTAGACATTTTAACAAGATTTGTAAAAG
AATATACTGGATTACCAGCAGAGCGTGTTATCGGTTCAGGTACTGTATTGGACAGTGCACGTTTACAATATTTAATTAGC
CAAGAACTTGGTGTTGCACCTTCAAGTGTTGACGCTAGTATTATTGGCGAGCATGGTGATACTGAACTTGCAGTTTGGTC
ACAAGCAAATGTAGCAGGTATTTCAGTATATGACACATTAAAAGAACAAACTGGTAGCGAAGCTAAAGCGGAAGAAATTT
ATGTGAATACACGTGACGCTGCTTATGAAATTATCCAAGCTAAAGGGTCAACATACTATGGTATTGCATTAGCATTGATG
CGCATTTCAAAAGCCATTTTAAATAATGAAAATAATGTCTTAAATGTTTCTATACAATTAGATGGTCAATATGGTGGTCA
CAAAGGCGTTTACCTAGGTGTACCAACATTAGTTAACCAACATGGCGCAGTTAAAATTTATGAAATGCCATTAAGTGCCG
AAGAACAAGCGTTGTTCGATAAATCTGTTAAAACATTAGAAGATACATTTGATTCAATTAAATATTTATTAGAAGACTAA

Upstream 100 bases:

>100_bases
TATTATAATAAAATAAAATTGTTAATCTTTAATTTCAGTATAGATATTTTTACGTGTAGTCACGTGTAAAATAAATTCAA
TTAGGTTAGGAGACATAATT

Downstream 100 bases:

>100_bases
GCCTATTTTAAGTATTAATTAGAAATATATTAATGGTAAGAGGATCTATTAGTGTTGCAGTAACACGTGGCACTGATAGG
TCCATTTTTTTAGCAAGCAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED

Sequences:

>Translated_319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED
>Mature_319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED

Specific function: Contributes to S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells, despite playing a secondary role in this resistance mechanism [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=39.672131147541, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=39.672131147541, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=38.3116883116883, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=38.3116883116883, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=36.4217252396166, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=36.4217252396166, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=36.6559485530547, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=36.6559485530547, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=36.6559485530547, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI260099725, Length=229, Percent_Identity=38.8646288209607, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI260099727, Length=211, Percent_Identity=40.7582938388626, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI207028494, Length=308, Percent_Identity=30.8441558441558, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI103472011, Length=287, Percent_Identity=25.0871080139373, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=28.4153005464481, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789632, Length=319, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=296, Percent_Identity=38.8513513513513, Blast_Score=216, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI17554310, Length=255, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322765, Length=230, Percent_Identity=26.5217391304348, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17136226, Length=318, Percent_Identity=36.7924528301887, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45550422, Length=304, Percent_Identity=29.9342105263158, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34420; Mature: 34420

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSP
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCC
VDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLISQELGVAPSSVDASIIGEHGD
ECCHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
TELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
CEEEEEECCCEEEEHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHH
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFD
HHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECHHHHCCCCCEEEEECCCCCHHHHHHH
KSVKTLEDTFDSIKYLLED
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSP
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCC
VDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLISQELGVAPSSVDASIIGEHGD
ECCHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
TELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
CEEEEEECCCEEEEHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHH
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFD
HHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECHHHHCCCCCEEEEECCCCCHHHHHHH
KSVKTLEDTFDSIKYLLED
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA