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The map label for this gene is cap5O [H]

Identifier: 57651167

GI number: 57651167

Start: 167489

End: 168751

Strand: Direct

Name: cap5O [H]

Synonym: SACOL0150

Alternate gene names: 57651167

Gene position: 167489-168751 (Clockwise)

Preceding gene: 57651166

Following gene: 57651168

Centisome position: 5.96

GC content: 34.36

Gene sequence:

>1263_bases
ATGAAGTTAACAGTAGTTGGCTTAGGTTATATTGGTTTACCAACATCAATTATGTTTGCAAAACATGGCGTCGATGTGCT
TGGTGTTGATATTAATCAGCAAACGATTGATAAGTTACAAAGTGGTCAAATTAGTATTGAAGAACCTGGATTACAAGAGG
TTTATGAAGAGGTACTGTCATCGGGAAAATTGAAGGTATCTACAACGCCAGATGCATCTGATGTTTTTATCATTGCCGTT
CCGACGCCGAATAATGATGATCAGTACCGGTCATGTGACATTTCGCTAGTTATGCGTGCATTAGATAGTATTTTATCATT
TTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTG
AAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAA
GAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACATT
CGTTCAGGGAGAAATGATTGAAACAGATGCACGTACTGCTGAAATGAGTAAGCTAATGGAAAACACATATAGAGACGTGA
ACATTGCTTTAGCTAATGAATTAACAAAAATTTGCAATAACTTAAATATTAATGTATTAGATGTGATTGAAATGGCAAAC
AAACATCCGCGTGTTAACATCCATCAGCCTGGTCCAGGTGTAGGCGGTCATTGTTTAGCTGTTGATCCGTACTTTATTAT
TGCTAAAGACCCTGAAAATGCAAAGTTAATTCAAACTGGACGTGAAATTAATAATTCAATGCCGGCCTATGTTGTTGATA
CAACGAAGCAAATCATCAAAGTGTTGAGCGGGAATAAAGTCACAGTATTTGGTTTAACTTATAAAGGTGATGTTGATGAT
ATAAGAGAATCACCAGCATTTGATATTTATGAGCTATTAAATCAAGAACCAGACATAGAAGTATGTGCTTATGATCCACA
TGTTGAATTAGATTTTGTGGAACATGATATGTCACATGCTGTCAAAGACGCATCGCTAGTATTGATTTTAAGTGACCACT
CAGAATTTAAAAATTTATCGGACAGTCATTTTGATAAAATGAAGCATAAAGTGATTTTTGATACAAAAAATGTTGTGAAA
TCATCATTTGAAGATGTATCGTATTATAATTATGGCAATATATTTAATTTTATCGACAAATAA

Upstream 100 bases:

>100_bases
TTATTGCGGACATCATGGATGAAACGACAACCAAAGATAAGGCATAAGTCATCTATTAAATAAAATCAACATACAAATCG
TTTTATTTGGAGGTTATAGT

Downstream 100 bases:

>100_bases
AATGTGTCAAACTAGGGCATACATGATTAAGGAAAGATAAGCTGTCATGTGTTTGAACTTCAGAGAGGATAATGTTATGA
AAAAAATTATGGTTATTTTC

Product: capsular polysaccharide biosynthesis protein Cap5O

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK

Sequences:

>Translated_420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK
>Mature_420_residues
MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAV
PTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDD
IRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVSYYNYGNIFNFIDK

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=451, Percent_Identity=23.7250554323725, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=41.9047619047619, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788340, Length=419, Percent_Identity=25.0596658711217, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560350, Length=462, Percent_Identity=23.5930735930736, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17136908, Length=446, Percent_Identity=22.1973094170404, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46801; Mature: 46801

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
CEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEECCCCHHHHHHHHHC
SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
CCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
CCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEECHHHHHHHHHH
KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCHHHHCCHHHCCCCCEEEEHHHHHHHH
VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
HHCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
HCCCEEEEEEECCHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEECCCCHHHHHCC
>Mature Secondary Structure
MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
CEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEECCCCHHHHHHHHHC
SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
CCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
CCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEECHHHHHHHHHH
KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCHHHHCCHHHCCCCCEEEEHHHHHHHH
VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
HHCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
HCCCEEEEEEECCHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA