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The map label for this gene is gidA [H]

Identifier: 57651105

GI number: 57651105

Start: 2805162

End: 2807039

Strand: Reverse

Name: gidA [H]

Synonym: SACOL2737

Alternate gene names: 57651105

Gene position: 2807039-2805162 (Counterclockwise)

Preceding gene: 57651106

Following gene: 57651104

Centisome position: 99.92

GC content: 35.3

Gene sequence:

>1878_bases
GTGGTTCAAGAATATGATGTAATCGTTATAGGTGCGGGACATGCAGGTGTAGAAGCAGGTTTAGCATCTGCAAGACGTGG
TGCTAAAACATTAATGCTAACAATAAATTTAGATAATATTGCATTTATGCCATGTAACCCATCTGTAGGTGGACCAGCTA
AAGGTATCGTTGTTCGTGAAATTGATGCTTTAGGTGGACAAATGGCAAAAACAATCGATAAAACACACATTCAAATGAGA
ATGTTAAATACAGGTAAAGGACCTGCTGTAAGAGCACTAAGAGCGCAAGCAGATAAAGTACTTTATCAACAAGAAATGAA
ACGCGTGATTGAAGATGAAGAAAATTTGCATATAATGCAAGGTATGGTAGACGAACTTATTATAGAAGATAATGAAGTTA
AAGGTGTACGTACAAATATTGGTACAGAGTATTTATCTAAAGCAGTAATTATTACAACGGGAACATTTTTACGTGGTGAA
ATCATTTTAGGTAATATGAAGTATTCAAGTGGACCAAATCACCAATTACCATCAATCACATTATCAGACAATTTAAGAGA
ACTTGGTTTTGATATTGTTCGTTTTAAAACAGGTACACCACCGCGTGTAAATTCAAAAACAATTGACTATTCGAAGACTG
AAATACAACCAGGTGACGATGTAGGTCGTGCATTCAGCTTTGAAACAACAGAATATATATTAGATCAATTGCCATGTTGG
CTAACGTATACTAATGCTGAAACACACAAAGTTATCGATGATAATTTACATCTATCTGCAATGTATTCAGGGATGATTAA
AGGAACCGGGCCACGTTATTGCCCTTCAATTGAAGATAAATTTGTTCGATTTAATGATAAGCCGCGACATCAACTTTTCT
TAGAGCCTGAAGGTCGTAATACAAATGAAGTATATGTGCAAGGATTGTCTACAAGTCTTCCTGAACATGTGCAACGTCAA
ATGTTAGAGACGATACCAGGTCTTGAAAAAGCAGATATGATGCGTGCCGGCTACGCAATTGAATATGATGCGATTGTGCC
AACGCAGTTATGGCCTACACTTGAAACGAAAATGATTAAAAACTTATATACTGCAGGTCAAATTAATGGTACATCTGGTT
ATGAAGAAGCAGCAGGACAAGGATTGATGGCAGGTATTAACGCTGCAGGTAAAGTGTTAAACACAGGCGAAAAGATATTA
AGTCGTTCAGATGCATATATTGGTGTCTTAATCGATGATCTTGTAACTAAAGGTACTAATGAACCTTATCGTTTACTAAC
ATCACGTGCAGAATATCGTTTGTTACTACGTCATGATAATGCTGATTTGAGATTGACGGATATGGGATATGAACTTGGTA
TGATTTCTGAAGAAAGATATGCACGTTTTAATGAAAAACGTCAGCAAATTGATGCGGAAATTAAGCGTTTATCAGATATT
CGTATTAAACCAAACGAACATACGCAAGCGATTATTGAACAACATGGTGGTTCTCGCTTAAAAGATGGTATTTTAGCTAT
CGATTTATTACGCAGACCTGAAATGACTTACGATATAATTTTAGAACTTTTAGAAGAAGAACATCAATTGAATGCAGATG
TTGAAGAACAAGTAGAAATACAAACAAAATATGAAGGTTATATCAATAAATCACTACAACAAGTTGAGAAAGTTAAGCGT
ATGGAAGAGAAGAAAATTCCAGAAGACTTAGATTATAGTAAGATTGATAGTTTGGCGACTGAAGCGCGAGAAAAATTATC
AGAAGTAAAACCTTTAAATATTGCACAAGCTTCTAGAATATCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTT
ACTTAGAACAAGGTAAACTCCAAAGGGTGAGTGACTAA

Upstream 100 bases:

>100_bases
TCAGTTATTCAGTCAATTCTGCTTAGGTAAATAGTAATTGAAATAGACGGAATACCGTCTTAAGAAGGCTAGTAAGATAT
CAAATAAGGAGGTTTATATT

Downstream 100 bases:

>100_bases
TGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTCAATTAACTGAGACTCAAAAACAACAGTTTCAAACA
TATTATCGTTTACTTGTTGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD

Sequences:

>Translated_625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD
>Mature_625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=42.2222222222222, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=40.5487804878049, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=39.7014925373134, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=52.0128824476651, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=40.8064516129032, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321202, Length=624, Percent_Identity=42.4679487179487, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=42.9245283018868, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70117; Mature: 70117

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCEEEEE
IDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQ
HHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSIT
HHHHHHHCCCCCCCCHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCEEE
LSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCE
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRN
EEECCCCCCEEECCCCEEEHHHHCHHCCCCCCCCCCCCHHHEECCCCCCCEEEECCCCCC
TNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIK
CCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
NLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTN
HHHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHCCHHHHCCCCCEEEEEHHHHHHCCCC
EPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
CHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEI
EECCCHHHHHHHHHCCCCHHHCCHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEE
QTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SGVNPADISILLIYLEQGKLQRVSD
CCCCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCEEEEE
IDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQ
HHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSIT
HHHHHHHCCCCCCCCHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCEEE
LSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCE
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRN
EEECCCCCCEEECCCCEEEHHHHCHHCCCCCCCCCCCCHHHEECCCCCCCEEEECCCCCC
TNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIK
CCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
NLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTN
HHHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHCCHHHHCCCCCEEEEEHHHHHHCCCC
EPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
CHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEI
EECCCHHHHHHHHHCCCCHHHCCHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEE
QTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SGVNPADISILLIYLEQGKLQRVSD
CCCCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA