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The map label for this gene is ydaP [H]

Identifier: 57651049

GI number: 57651049

Start: 2611338

End: 2613077

Strand: Reverse

Name: ydaP [H]

Synonym: SACOL2553

Alternate gene names: 57651049

Gene position: 2613077-2611338 (Counterclockwise)

Preceding gene: 57651050

Following gene: 57651048

Centisome position: 93.01

GC content: 34.43

Gene sequence:

>1740_bases
ATGGCAAAAATAAAAGCAAATGAAGCATTAGTTAAAGCATTACAAGCATGGGATATAGATCACTTGTATGGTATTCCAGG
AGACTCAATCGACGCAGTAGTCGATAGTTTACGTACAGTGAGAGATCAATTTAAATTTTATCATGTACGTCATGAAGAAG
TAGCAAGCTTAGCGGCTGCTGGTTACACAAAATTAACTGGTAAAATCGGTGTGGCATTAAGTATCGGTGGCCCTGGTTTA
ATTCATTTATTAAATGGTATGTATGATGCCAAAATGGATAATGTACCGCAATTAATATTATCTGGACAAACGAATAGTAC
AGCACTTGGAACGAAAGCATTCCAAGAAACAAATTTACAAAAATTATGTGAAGATGTAGCCGTTTATAATCACCAAATTG
AAAAAGGTGACAATGTGTTTGAAATCGTTAACGAAGCAATTCGTACGGCATATGAACAAAAAGGTGTAGCTGTTGTTATT
TGTCCTAACGACTTATTAACTGAAAAAATTAAAGATACAACGAATAAACCAGTAGATACATCAAGACCAACAGTAGTATC
ACCAAAATATAAAGACATCAAAAAAGCGGTTAAACTAATTAATAAAAGTAAAAAGCCTGTCATGTTAATTGGTGTAGGTG
CGAAACATGCGAAAGATGAGCTACGTGAATTTATTGAAATGGCTAAAATTCCTGTCATTCATTCATTACCAGCTAAAACA
ATCTTGCCGGATGATCATCCATATAGTATCGGTAACTTAGGTAAAATCGGTACCAAAACATCTTATCAAACAATGCAGGA
AGCGGATTTATTAATTATGGTTGGTACAAACTATCCATATGTGGATTACTTACCTAAGAAAAATATTAAAGCCATTCAAA
TTGACACAAATCCTAAAAATATCGGACATCGTTTCAATATTAATGTAGGAATTGTTGGAGATAGTAAAATTGCGTTGCAT
CAGTTAACTGAAAATATTAAACATGTTGCTGAAAGACCATTCTTAAACAAAACGTTAGAACGTAAAGCGGTTTGGGATAA
ATGGATGGAACAAGATAAAAATAATAATAGTAAACCATTACGTCCAGAACGATTAATGGCATCAATCAATAAATTTATTA
AAGATGATGCAGTGATTTCAGCAGATGTAGGTACAGCAACAGTTTGGTCAACTCGATACTTAAACCTTGGTGTAAATAAC
AAGTTCATCATTTCAAGTTGGTTAGGTACAATGGGTTGCGGTCTTCCAGGTGCAATTGCATCAAAAATTGCATATCCAAA
TAGACAAGCCATCGCAATTGCTGGTGACGGTGCATTCCAAATGGTAATGCAAGACTTCGCTACAGCAGTACAATATGATT
TACCTTTAACTGTATTTGTACTTAATAACAAACAGTTAGCATTTATTAAATATGAACAACAAGCAGCTGGTGAATTAGAA
TATGCAGTTGATTTTTCTGATATGGATCATGCAAAATTTGCTGAGGCAGCAGGTGGTAAAGGTTATACAATTAAGAGTGC
TAGCGAAGTAGATGCTATAGTCGAAGAGGCATTAGCACAAGATGTACCAACGATTGTAGATGTATATGTTGATCCTAATG
CTGCGCCATTACCAGGTAAAATTGTAAATGAAGAAGCGCTTGGTTATGGTAAGTGGGCATTTAGATCAATTACTGAAGAT
AAACATTTAGATTTAGATCAAATTCCACCAATTTCAGTGGCAGCAAAACGTTTCTTATAA

Upstream 100 bases:

>100_bases
ACTGCAGTTATTAGTTCAGTGTTAATACCTGTTCTAATTTTATTATTCTATTAATTTAGATATTTAAAATGATAGACAGA
AAGGGAGGCTATTAGTAATA

Downstream 100 bases:

>100_bases
CTGATTTAAAGGTTATCACAATTGAATTGAACTATAAAAACGGTAATTTCTATTTCAACAAAATGGGAATTGCCGTTTTG
TTTATTTATCACAAATGATC

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGL
IHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALH
QLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNN
KFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITED
KHLDLDQIPPISVAAKRFL

Sequences:

>Translated_579_residues
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGL
IHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALH
QLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNN
KFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITED
KHLDLDQIPPISVAAKRFL
>Mature_578_residues
AKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLI
HLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVIC
PNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKTI
LPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQ
LTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNNK
FIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELEY
AVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDK
HLDLDQIPPISVAAKRFL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=22.7758007117438, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=32.7898550724638, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=29.3594306049822, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=26.7889908256881, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=25.4867256637168, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=24.7311827956989, Blast_Score=137, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=22.3214285714286, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=22.3214285714286, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=585, Percent_Identity=25.2991452991453, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=588, Percent_Identity=28.4013605442177, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.822695035461, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321524, Length=559, Percent_Identity=23.613595706619, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320588, Length=532, Percent_Identity=22.3684210526316, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=527, Percent_Identity=21.0626185958254, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=25.7548845470693, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 63758; Mature: 63627

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAA
CCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHC
GYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQ
CCCEECCCEEEEEEECCCHHHHHHCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCHH
KLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
SRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
CCCCEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCC
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKN
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCC
IGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNNKFIISSWLGTMGCGLPGAIA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCEEHHHHHHHHCCCCCHHHH
SKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHEECCCEEEEEECCCEEEEEEECCCCCCCEE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGK
EEEECCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
IVNEEALGYGKWAFRSITEDKHLDLDQIPPISVAAKRFL
CCCHHHCCCCHHHHHHCCCCCCCCHHCCCCHHHHHHHCC
>Mature Secondary Structure 
AKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAA
CCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHC
GYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQ
CCCEECCCEEEEEEECCCHHHHHHCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCHH
KLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
SRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
CCCCEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCC
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKN
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCC
IGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNNKFIISSWLGTMGCGLPGAIA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCEEHHHHHHHHCCCCCHHHH
SKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHEECCCEEEEEECCCEEEEEEECCCCCCCEE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGK
EEEECCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
IVNEEALGYGKWAFRSITEDKHLDLDQIPPISVAAKRFL
CCCHHHCCCCHHHHHHCCCCCCCCHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]