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The map label for this gene is femX [H]

Identifier: 57650846

GI number: 57650846

Start: 2319781

End: 2321046

Strand: Reverse

Name: femX [H]

Synonym: SACOL2253

Alternate gene names: 57650846

Gene position: 2321046-2319781 (Counterclockwise)

Preceding gene: 57650847

Following gene: 57650845

Centisome position: 82.62

GC content: 33.89

Gene sequence:

>1266_bases
ATGGAAAAGATGCATATCACTAATCAGGAACATGACGCATTTGTTAAATCCCACCCAAATGGAGATTTATTACAATTAAC
GAAATGGGCAGAAACAAAGAAATTAACTGGATGGTACGCGCGAAGAATCGCTGTAGGTCGTGACGGTGAAGTTCAGGGTG
TTGCGCAGTTACTTTTTAAAAAAGTACCTAAATTACCTTATACGCTATGTTATATTTCGCGTGGTTTTGTTGTTGATTAT
AGTAATAAAGAAGCGTTAAATGCATTGTTAGACAGTGCAAAAGAAATTGCTAAAGCTGAGAAAACGTATGCAATTAAAAT
CGATCCTGATGTTGAAGTTGATAAAGGTACAGATGCTTTGCAAAATTTGAAAGCGCTTGGTTTTAAACATAAAGGATTTA
AAGAAGGTTTATCAAAAGACTACATCCAACCACGTATGACTATGATTACACCAATTGATAAAAATGATGATGAGTTATTA
AATAGTTTTGAACGCCGAAATCGTTCAAAAGTGCGCTTGGCTTTAAAGCGAGGTACGACAGTAGAACGATCTGATAGAGA
AGGTTTAAAAACATTTGCTGAGTTAATGAAAATCACTGGGGAACGCGATGGCTTCTTAACGCGTGATATTAGTTACTTTG
AAAATATTTATGATGCGTTGCATGAAGATGGAGATGCTGAACTATTTTTAGTAAAGTTGGATCCAAAAGAAAATATAGCG
AAAGTAAATCAAGAATTGAATGAACTTCATGCCGAAATTGCTAAATGGCAGCAGAAGATGAAAACATCTGAAAAGCAAGC
TAAAAAAGCGCAAAATATGATTAATGATGCGCAAAATAAAATTGCTAAAAATGAAGATTTAAAACGAGACCTAGAAGCTT
TAGAAAAGGAACATCCTGAAGGTATTTATCTTTCTGGTGCACTATTAATGTTTGCTGGCTCAAAATCATATTACTTATAT
GGTGCGTCTTCTAATGAATTTAGAGATTTTTTACCAAATCATCATATGCAGTATACGATGATGAAGTATGCACGTGAACA
TGGTGCAACAACTTACGATTTCGGTGGTACAGATAATGATCCAGATAAAGACTCAGAACATTATGGATTATGGGCATTTA
AAAAAGTGTGGGGAACATACTTAAGTGAAAAGATTGGTGAATTTGATTATGTATTGAATCAGCCATTGTACCAATTAATT
GAGCAAGTTAAACCGCGTTTAACAAAAGCTAAAATTAAAATATCTCGTAAATTAAAACGAAAATAG

Upstream 100 bases:

>100_bases
AAAATAAGAGTTCAAATCTTACACAAGCTCTGAATCGACACTATAAGATACAAACTGTATAATTAAAGGTATTGTTAAAT
AGAAGGAGATATCATAAATC

Downstream 100 bases:

>100_bases
ATTAACGACTGAAATCTGAACGCTCATAAGACTGTCATTTGCGTTCAGATTTTTTTACACAATATAGAATGGTTGAGTAA
AATATTTTTGAATATAGTGA

Product: femX protein

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance X [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDY
SNKEALNALLDSAKEIAKAEKTYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIA
KVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLY
GASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLI
EQVKPRLTKAKIKISRKLKRK

Sequences:

>Translated_421_residues
MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDY
SNKEALNALLDSAKEIAKAEKTYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIA
KVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLY
GASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLI
EQVKPRLTKAKIKISRKLKRK
>Mature_421_residues
MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDY
SNKEALNALLDSAKEIAKAEKTYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIA
KVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLY
GASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLI
EQVKPRLTKAKIKISRKLKRK

Specific function: Catalyzes the incorporation of the first glycine of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. This glycine is added to the epsilon-amino group of the L-lysine of the membrane-bound lipid II intermediate (

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 48552; Mature: 48552

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFK
CCCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKTYAIKIDPDVEVDKGTDAL
HCCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHH
QNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELLNSFERRNRSKVRLALKRGTT
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHEEEHHHCCCC
VERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIA
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHH
KVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
GIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDND
CEEEECEEEEEECCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
PDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLIEQVKPRLTKAKIKISRKLKR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFK
CCCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKTYAIKIDPDVEVDKGTDAL
HCCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHH
QNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELLNSFERRNRSKVRLALKRGTT
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHEEEHHHCCCC
VERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIA
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHH
KVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
GIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDND
CEEEECEEEEEECCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
PDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLIEQVKPRLTKAKIKISRKLKR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA