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The map label for this gene is mraW [H]

Identifier: 57650291

GI number: 57650291

Start: 1196309

End: 1197244

Strand: Direct

Name: mraW [H]

Synonym: SACOL1192

Alternate gene names: 57650291

Gene position: 1196309-1197244 (Clockwise)

Preceding gene: 57650290

Following gene: 57650292

Centisome position: 42.58

GC content: 36.11

Gene sequence:

>936_bases
GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAAAATGGTGTGTACATTGACTG
TACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAATCAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAG
ACCAAACTGCAATTGATAATGCTAAAGAGGTATTAAAGGATCATTTGCATAGGGTGACTTTTGTTCATAGCAACTTCCGT
GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGACTTGGGTGTTTCAAGCCCACA
ACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGACGCAACATTAGACATGCGTATGGACCAAACACAAGAACTAA
CAGCATATGAAATTGTTAACAATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTAGAATTAGTTGACATTATAAA
AGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACATCCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAA
ACGATGAATTGTCAGCTTTTGAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGATGGCAGGATTTCGGTAATC
ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGTCCAGAGGTACCAAGAGGATT
ACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGTGTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAG
ATGACAATAACAGAGCACGAAGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA

Upstream 100 bases:

>100_bases
TAGAGAAACTTGGAATGATTTCTATGAAGAATCTGAAGAAAGTTTCGAAGATATTGCTGAAGATTTAATAGATTTTGATT
TTTAAAATGGAGGAATTGAA

Downstream 100 bases:

>100_bases
GGAGCAATTTATAATGGCTGTAGAAAAAGTGTACCAACCATATGACGAACAAGTTTATAATAGTATACCGAAGCAACAAC
CACAAACTAAGCCCGAAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

Sequences:

>Translated_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK
>Mature_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=33.1378299120235, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=36.8421052631579, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=41.4012738853503, Blast_Score=245, Evalue=2e-66,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=35.1681957186544, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=35.1681957186544, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35710; Mature: 35710

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKEVLKDHLHRVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC
>Mature Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKEVLKDHLHRVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA