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The map label for this gene is yfkN [H]

Identifier: 57650216

GI number: 57650216

Start: 1040542

End: 1042053

Strand: Direct

Name: yfkN [H]

Synonym: SACOL1031

Alternate gene names: 57650216

Gene position: 1040542-1042053 (Clockwise)

Preceding gene: 57650215

Following gene: 57650218

Centisome position: 37.04

GC content: 32.14

Gene sequence:

>1512_bases
ATGGAAAAAAATGAAAACATTAATGTAGAGATTTTAACTACGTCAGATATGCATAGTCATTTCTTAAATGGTGATTATGG
TTCAAATATTTATAGAGCTGGTACTTATGTTAACCAAGTAAGAGCACAAAATCATCGCGTCATTTTATTAGATAGTGGCG
GAAGTTTAGCTGGCTCGTTAGCGGCCTATTATTATGCTATTGTTGCACCTTATAAACGACATCCAATGATAAAGTTAATG
AACAGAATGCATTATGATGCTAGCGGTGTGAGTCCAAGTGAATTCAAGTTTGGTTTATCATTTTTAACTCGTTCAATTGC
TTTGGCACGTTTTCCATGGTTATCAGCAAATATTGAATACAATGTTACTAAGGAGCCTTATTTTTCAACTCCATATTGTA
TTAAACATTTTGGTGACTTAAAAATTGCTATCGTAGGCGTCACAGCAGATGGTTTAATGGAAAATGAGTATTCTGAAATG
GAGCAAGATGTATCTATTGAAAAGACATTAGTGGCATCAAAACGTTGGATTAGATATATCCATGAAGTTGAAGAGCCAGA
TTTTTTGATTGTAATTTATCATGGTGGATTGAATAAAATTAGTAATAGTACGAAAAATAAAAAGGCAAGTTCGAATGAAG
CTGAAAAATTAATGGAAGAACTCGGTGTTATAGATTTAATGATTACAGCTCATCAGCATCAAACAATAGTAGGTCAAGAT
CATGAAACGTATTATGTTCAGGCTGGTCAAGATGCCAAAGAGCTTGTACATCTTTCGATTAATTTTAAAAAGAGAACAAC
AACTTATGATGTTGAAAGCATTGATTCTAAAGTGATTGACTTAAATGAGTATGAAGAGGATCAAGAATTATTAGATTTAA
CATTCTATGATAGAAAAGCAGTGGCTTATTGGTCACAGGAAATCATAAGTGATAAAGGTTTGATGTTATCAGTAAATGGG
TTACAAGATTTAGTCTGTCAAACACATCCATTTTCGCAATTATTACATGATGCAATTCACCTTGCATTTGATAATGATAT
AACATGTGTCCACGTGCCTATGAACGGAGAGAAGGGGTTGAGTGGACAGATTCGAAATGAAGATTTGTATCATGCATACC
CATATCCAGATAAGCCAATGGATATGACAATTAGTGGTCAAAATATCAAAGATATATTGGAGTATAGTTATTCACATTTA
GATTTTGTTAACGAGCAATTAAGCTTAACAATTATTGATGAAACGTTATGTACAATGTGGCAAGGATTCAATTATGAGAT
TGATATGAATCAAGAACCTGGGCAACGAGTAATGTTAGATCAAATTGATTTGACTAAGAGTTATAGAGTTACAATGACTG
ACTATTGTTATCGTAACTACAAGAATTATTTAAAAAATGCTATTATACATGAATCATACGATGAAACAATGAGTACATTA
ATTGCAGAGAAGTTAAGAGATCCGAATTATCGTATTTCATGTAGTGATAATTTTATAGTTAAAAACAGGTAA

Upstream 100 bases:

>100_bases
CTGGAACTTAGATTGCTTTTTTAGTTTGTATTTTTAACTTATTTTATAAGACGATTGGTTTCGAAAATGGTAAACTAGTA
ACAATGAGAGGTGTAACATG

Downstream 100 bases:

>100_bases
ATATTGCTATGTAATAAATTAATTATAAGTTCAACAAGTATAAAAACCTTTCTTAGAGAAGATTATCTAGGAAAGGTTTT
TAGAATTTTAAAATTGATAT

Product: 5' nucleotidase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLM
NRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQD
HETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNG
LQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHL
DFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTL
IAEKLRDPNYRISCSDNFIVKNR

Sequences:

>Translated_503_residues
MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLM
NRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQD
HETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNG
LQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHL
DFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTL
IAEKLRDPNYRISCSDNFIVKNR
>Mature_503_residues
MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLM
NRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQD
HETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNG
LQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHL
DFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTL
IAEKLRDPNYRISCSDNFIVKNR

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=252, Percent_Identity=24.6031746031746, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786687, Length=200, Percent_Identity=30.5, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 57928; Mature: 57928

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSL
CCCCCCEEEEEEEECCHHHHHCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCCCHHHH
AAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEY
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCEEE
NVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRYI
EECCCCCCCCCHHHHHCCCEEEEEEEEECCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHH
HEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQD
HHCCCCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECCC
HETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKA
CCEEEEECCCCHHHEEEEEEEEEECCEEEEHHHCCCEEEECCCCCCCHHHEEEEEECCHH
VAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGL
HHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHEEEECCCEEEEEECCCCCCCC
SGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTMW
CCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
QGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTL
CCCCEEEECCCCCCCEEEEEHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IAEKLRDPNYRISCSDNFIVKNR
HHHHHCCCCEEEEECCCEEEECC
>Mature Secondary Structure
MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSL
CCCCCCEEEEEEEECCHHHHHCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCCCHHHH
AAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEY
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCEEE
NVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRYI
EECCCCCCCCCHHHHHCCCEEEEEEEEECCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHH
HEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQD
HHCCCCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECCC
HETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKA
CCEEEEECCCCHHHEEEEEEEEEECCEEEEHHHCCCEEEECCCCCCCHHHEEEEEECCHH
VAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGL
HHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHEEEECCCEEEEEECCCCCCCC
SGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTMW
CCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
QGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTL
CCCCEEEECCCCCCCEEEEEHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IAEKLRDPNYRISCSDNFIVKNR
HHHHHCCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]