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The map label for this gene is appA [H]

Identifier: 57650184

GI number: 57650184

Start: 1003533

End: 1005248

Strand: Direct

Name: appA [H]

Synonym: SACOL0996

Alternate gene names: 57650184

Gene position: 1003533-1005248 (Clockwise)

Preceding gene: 57650183

Following gene: 57650185

Centisome position: 35.72

GC content: 32.52

Gene sequence:

>1716_bases
ATGGGGAAGCTAATTAAATATATTTCAATACTTCTTATTGTCGTTTTAGTGTTGAGTGCTTGCGGAAAAAGCAGTAATAA
AGATGAAGGAGTAAAAGATGCTACTAAAACGGAAACCTCAAAACATAAAGGTGGTACCTTAAATGTAGCATTAACAGCAC
CGCCAAGTGGTGTTTATTCTTCGTTATTAAATAGTACACATGCAGATTCTGTAGTTGAGGGATATTTTAACGAAAGCTTA
TTAGCAACTGATAAAAAAATACGTCCTAAGGCATATATTGCTTCATGGAAGGACATCGAGCCGGCTAAGAAAATAGAATT
TAAAATTAAAAAAGGTATTAAATGGCATGATGGTAATGAATTGAAAATTGATGATTGGATTTATTCAATTGAAGTCTTAG
CTAACAAGGACTACGAAGGTGCTTATTATCCAAGTGTAGAAAATATCCAAGGTGCGAAAGATTATCATGAAGGAAAAACT
GATCATATTAGCGGATTGAAGAAAATAGATGACTACACTATGCAGGTTACATTTGATAAAAAACAAGAAAATTACTTAAC
AGGATTTATTACTGGACCTTTATTAAGTAAAAAATATTTATCAGATGTACCAATTAAAGATTTAGCGAAATCAGATAAAA
TCCGAAAATATCCTATTGGTATTGGACCGTATAAAGTTAAGAAAATCGTTCCAGGTGAGGCTGTTCAACTCGTTAAATTT
GATGATTATTGGCAAGGTAAGCCTGCACTAGACAAAATCAATTTAAAAGTTATTGATCAAGCGCAAATTATTAAGGCAAT
GGAAAAAGGCGATATTGATGTTGCGAATGATGCTACCGGTGCAATGGCAAAAGATGCTAAGTCATCTAATGCTGGTCTCA
AGGTATTATCTGCGCCAAGCTTAGACTACGGTTTAATAGGATTCGTATCTCATGATTACGATAAAAAAGCTAATAAAACT
GGTAAAGTGAGACCAAAATATGAAGACAAAGAATTACGTAAAGCAATGCTTTATGCAATTGATAGAGAAAAATGGATCAA
AGCGTTTTTCAATGGTTACGCTAGTGAAATCAATAGTTTTGTACCATCTATGCATTGGATAGCAGCCAATCCTAAGGACC
TAAATGATTACAAATATGATCCTGAAAAAGCTAAAAAAATCTTAGATAAGTTAGGTTATAAAGATAGAGATGGTGACGGA
TTTAGAGAAGATCCTAAAGGTAATAAATTTGAGATTAACTTTAAACATTATTCAGGTTCTAATCCTACTTTTGAACCAAG
AACTGCTGCGATAAAAGATTTCTGGGAAAAAGTTGGCTTGAAAACAAATGTGAAGTTAGTAGAATTCGGTAAATATAATG
AAGACTTAGCAAATGCATCTAAAGATATGGAAGTGTACTTCAGATCATGGGCAGGAGGTACAGATCCAGATCCATCAGAT
TTATACCACACTGATAGACCTCAAAATGAAATGAGAACAGTTTTACCAAAATCAGATCAATATTTAGATGATGCATTAGA
CTTCGAAAAAGTAGGCATTGATGAAAAGAAACGTAAAGATATTTATGTTAAATGGCAAAAATATATGAATGATGAGTTAC
CTGGATTACCAATGTTCCAAGGTAAATCGATAACTATTGTTAACGATAAAGTACGAAACTTAGACATTGAAATTGGAACT
GATCAAAGTTTATATAATTTAACTAAAGAAGCTTAG

Upstream 100 bases:

>100_bases
AATGCATAAATAACTGAATATTCTAAAAATAAACTTGTAATAATTTTTTCTATGAGTAAACTAAAAAGAAAAAATTAGAT
TGAAAGTAGGAGGCATATGT

Downstream 100 bases:

>100_bases
TAATGTAACCTCGACGATTTTGTCGAGGTTTACTTATATGGGGGGATGTTATGAATAATGTATTGTTAGAGGTTAAAGAT
TTAGAAACATCATTAAAAAT

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESL
LATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKF
DDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKT
GKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDG
FREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSD
LYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGT
DQSLYNLTKEA

Sequences:

>Translated_571_residues
MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESL
LATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKF
DDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKT
GKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDG
FREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSD
LYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGT
DQSLYNLTKEA
>Mature_570_residues
GKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLL
ATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTD
HISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFD
DYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTG
KVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGF
REDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSDL
YHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTD
QSLYNLTKEA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=474, Percent_Identity=25.7383966244726, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1787551, Length=414, Percent_Identity=18.5990338164251, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=22.1556886227545, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64577; Mature: 64446

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHH
SLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNE
HHHCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCEECCCCE
LKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTDHISGLKKIDDYTMQVTFDK
EEEHHHEEEEEEEECCCCCCCCCCCHHCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEECC
KQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKF
CCCCEEEEEEECCHHHHHHHCCCCHHHHHCCCCCEECCCCCCCEEEEEECCCCEEEEEEE
DDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPS
CCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCEECCCCCCHHHHCCCCCCCCEEEEECCC
LDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSF
CCCCEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHYSGS
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
NPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSD
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCC
LYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQ
CCCCCCCHHHHHHHCCCCCHHHHHHHCHHHCCCCCHHCCCEEEEEHHHCCCCCCCCCCCC
GKSITIVNDKVRNLDIEIGTDQSLYNLTKEA
CCEEEEECCCCEEEEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
GKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYS
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHH
SLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNE
HHHCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCEECCCCE
LKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTDHISGLKKIDDYTMQVTFDK
EEEHHHEEEEEEEECCCCCCCCCCCHHCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEECC
KQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKF
CCCCEEEEEEECCHHHHHHHCCCCHHHHHCCCCCEECCCCCCCEEEEEECCCCEEEEEEE
DDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPS
CCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCEECCCCCCHHHHCCCCCCCCEEEEECCC
LDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSF
CCCCEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHYSGS
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
NPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSD
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCC
LYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQ
CCCCCCCHHHHHHHCCCCCHHHHHHHCHHHCCCCCHHCCCEEEEEHHHCCCCCCCCCCCC
GKSITIVNDKVRNLDIEIGTDQSLYNLTKEA
CCEEEEECCCCEEEEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]