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The map label for this gene is eno [H]

Identifier: 57238683

GI number: 57238683

Start: 1733858

End: 1735102

Strand: Reverse

Name: eno [H]

Synonym: CJE1844

Alternate gene names: 57238683

Gene position: 1735102-1733858 (Counterclockwise)

Preceding gene: 57238684

Following gene: 57238682

Centisome position: 97.6

GC content: 37.59

Gene sequence:

>1245_bases
ATGTTAGTAATTGAAGATGTTAGAGCCTATGAAGTTCTTGATAGTAGAGGAAATCCAACCGTAAAAGCCGAAGTTACGCT
AAGCGATGGAAGTGTAGGTGCGGCCATAGTTCCAAGTGGTGCAAGTACAGGTTCAAAAGAAGCCTTAGAATTGCGTGATA
ATGATGAAAGATTTGGCGGAAAAGGCGTTTTAAAAGCTGTTGCAAATGTCAATGAGACCATTGCAGATGAAATTTTAGGA
CTAGATGCTTTTAACCAAACTCAACTTGATGATACTTTGCGTGAACTTGATGGAACAAATAATTACTCAAATTTAGGTGC
AAATGCGACTTTAGGTGTATCTATGGCAACAGCTCGTGCGGCTGCAGCTGCTTTGGGAATGCCTTTATATCGCTACTTAG
GTGGTGCAAATGCGAGCATTTTACCCGTGCCAATGTGTAATATCATAAATGGCGGTGCTCATGCAAACAACAATGTAGAT
TTTCAAGAATTTATGATCATGCCTTTTGGTTTTACTAGCTTTAAAGAAGCTTTGCGTTCAGTTTGTGAAATTTATGTGAT
ATTAAAAAAAGAACTCGCAAATTCTGGACATTCTACGGCTTTAGGTGATGAAGGCGGTTTTGCTCCAAATTTAGCTAACA
ACACAGAACCTATCGATCTTTTAATGACTTGTATCAAAAAAGCAGGTTATGAAAATCGTGTAAAAATCGCTCTTGATGTG
GCAAGTACAGAATTTTTCAAAGATGGCAAATATCACATGGAAGGCAAGGCTTTTTCAAGCGAGGATTTGATCGAACGCTA
TGTAGAACTTTGTGCAAAATATCCAATTTGCAGTATCGAAGATGGCTTAGCTGAAAATGACTTTGAAGGTTGGATTAAGC
TTACTGAAAAATTGGGCAATAAAATTCAGCTTGTAGGCGATGATTTGTTTGTAACTAATGAAGATATTTTAAGAGAAGGT
ATCATCAAAAAAATGGCAAATGCTGTGCTTATTAAACCAAATCAAATCGGAACTATTACCCAAACTATGAGAACTGTGCG
TTTAGCGCAAAGAAATAACTATAAATGTGTGATGAGTCATAGAAGCGGCGAAAGTGAAGATGCTTTTATAGCGGATTTTG
CTGTGGCTTTAAATACAGGACAAATCAAAACAGGAGCCCTAGCAAGAGGTGAAAGAACCGCAAAATACAATCGCTTGCTT
GAAATTGAACTTGAAAGCGATGAATACTTAGGAGAAAAGCTCTGA

Upstream 100 bases:

>100_bases
AAAACCCTGAAATTGCAGATGAAATCACAAAAGCAATTCAAAATTCTATGGGAATAGAAGGTATGATCAGCGGTAGCGAA
GATGACGAAGGAGAAGAATA

Downstream 100 bases:

>100_bases
GCGATTTACTTAAAGAATACGACGAAAGCACAAGAAAAAAAAGTTTTTATACCCATGTTATAAAAATGATTTTTTGGGCT
TTTCTTGTTGTAATTGGAGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDERFGGKGVLKAVANVNETIADEILG
LDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARAAAAALGMPLYRYLGGANASILPVPMCNIINGGAHANNNVD
FQEFMIMPFGFTSFKEALRSVCEIYVILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYENRVKIALDV
ASTEFFKDGKYHMEGKAFSSEDLIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGNKIQLVGDDLFVTNEDILREG
IIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSHRSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLL
EIELESDEYLGEKL

Sequences:

>Translated_414_residues
MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDERFGGKGVLKAVANVNETIADEILG
LDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARAAAAALGMPLYRYLGGANASILPVPMCNIINGGAHANNNVD
FQEFMIMPFGFTSFKEALRSVCEIYVILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYENRVKIALDV
ASTEFFKDGKYHMEGKAFSSEDLIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGNKIQLVGDDLFVTNEDILREG
IIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSHRSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLL
EIELESDEYLGEKL
>Mature_414_residues
MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDERFGGKGVLKAVANVNETIADEILG
LDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARAAAAALGMPLYRYLGGANASILPVPMCNIINGGAHANNNVD
FQEFMIMPFGFTSFKEALRSVCEIYVILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYENRVKIALDV
ASTEFFKDGKYHMEGKAFSSEDLIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGNKIQLVGDDLFVTNEDILREG
IIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSHRSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLL
EIELESDEYLGEKL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=419, Percent_Identity=47.9713603818616, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=48.4777517564403, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=48.4777517564403, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI4503571, Length=427, Percent_Identity=48.0093676814988, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=44, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI169201331, Length=493, Percent_Identity=21.9066937119675, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI169201757, Length=493, Percent_Identity=21.9066937119675, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI239744207, Length=493, Percent_Identity=21.9066937119675, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=54.3735224586288, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71995829, Length=420, Percent_Identity=49.5238095238095, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17536383, Length=420, Percent_Identity=49.5238095238095, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32563855, Length=181, Percent_Identity=46.9613259668508, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=422, Percent_Identity=47.8672985781991, Blast_Score=345, Evalue=6e-96,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=48.3720930232558, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=45.1536643026005, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324974, Length=423, Percent_Identity=45.1536643026005, Blast_Score=329, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6324969, Length=423, Percent_Identity=45.1536643026005, Blast_Score=329, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24580918, Length=420, Percent_Identity=47.8571428571429, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580916, Length=420, Percent_Identity=47.8571428571429, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580920, Length=420, Percent_Identity=47.8571428571429, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580914, Length=420, Percent_Identity=47.8571428571429, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI281360527, Length=420, Percent_Identity=47.8571428571429, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI17137654, Length=420, Percent_Identity=47.8571428571429, Blast_Score=354, Evalue=6e-98,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 44978; Mature: 44978

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDERFGG
CEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCCCCC
KGVLKAVANVNETIADEILGLDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARA
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHH
AAAALGMPLYRYLGGANASILPVPMCNIINGGAHANNNVDFQEFMIMPFGFTSFKEALRS
HHHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCCCCCCCCHHHEEEECCCHHHHHHHHHH
VCEIYVILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYENRVKIALDV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
ASTEFFKDGKYHMEGKAFSSEDLIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGN
CCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
KIQLVGDDLFVTNEDILREGIIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSH
EEEEEECCEEECCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEC
RSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLLEIELESDEYLGEKL
CCCCCCCCCCEEEEEEEECCCEECCCCCCCCHHHHCCEEEEEEECCCHHCCCCC
>Mature Secondary Structure
MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDERFGG
CEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCCCCC
KGVLKAVANVNETIADEILGLDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARA
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHH
AAAALGMPLYRYLGGANASILPVPMCNIINGGAHANNNVDFQEFMIMPFGFTSFKEALRS
HHHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCCCCCCCCHHHEEEECCCHHHHHHHHHH
VCEIYVILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYENRVKIALDV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
ASTEFFKDGKYHMEGKAFSSEDLIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGN
CCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
KIQLVGDDLFVTNEDILREGIIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSH
EEEEEECCEEECCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEC
RSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLLEIELESDEYLGEKL
CCCCCCCCCCEEEEEEEECCCEECCCCCCCCHHHHCCEEEEEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA