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The map label for this gene is sdaA [H]

Identifier: 57238636

GI number: 57238636

Start: 1692930

End: 1694294

Strand: Reverse

Name: sdaA [H]

Synonym: CJE1796

Alternate gene names: 57238636

Gene position: 1694294-1692930 (Counterclockwise)

Preceding gene: 57238637

Following gene: 57238632

Centisome position: 95.3

GC content: 34.65

Gene sequence:

>1365_bases
ATGAGTAATTTAAGCATTTTTAAAATAGGTGTTGGTCCTTCTTCTTCTCATACTTTAGGGCCTATGCTTGCTGGAAATTT
ATTTTGTAAAAAAGTCGCTAAAAAGCTTGATGAAATTGATAGAGTTGAGGTTACTCTTTATGGTTCTTTGTCATTAACAG
GTAAAGGACACTTGAGTGATAAGGCTGTAATTTGGGGGTTAAATGGCCTAGAAGCTAAAAATTTAAGCACAGCAATACAA
GATGAAGTAAATAAAAATGCTATTGAAAATGCTCAAATTGATTTTTGTGGTGAGAAAAAACTTTGTTTTAATTATGAAAA
AGATTTGATATTTTCCAAAGATTTTTTACCTTTACATGAGAATGGTATGAAGATTAAAGCTTATGATTGTAAAGGTGGGT
TAGTTGATGAAGAAACTTATTATTCTGTAGGCGGAGGCTTTGTTTTAACAGCTGCACAATTAGAAAAAAAAGGTAAAAAT
TCCAACCAAAATAAAAAGAAAAAACTAGATATAGAGCTTAACAATGCAAAAGAAGCTTTAGAGCTTTGCGATAAAAGAGA
TTGGGATTTAGCAGAGCTTTCTTATCGTTACGAATTGCAATTTCACACTAAAGAGGAAATTCGTGCTTATTGTCTTGAAA
TTTGGGAAGTAATGCAAGAAGTGTATTATAATGGCACTCATCCAAATGAAGATTATTTGCCTGGAAAACTTCATTTAAAG
CGTAGAGCTAAAGGACTTAAAGAAAGAGTGGCGATGACAGCTGATCCTATGGGCATTATCGATTTTATTTCTTTATATGC
TATTGCGATTGCTGAAGAAAATGCTAGCGGAGCAAAAGTTGTAACCGCACCAACAAATGGAGCATGTGCTGTTATCCCTG
CTGTTATGCTTTATCTTAAAAACCATACTATAGGTTTTAGTGATGAAAAGGCTATAGAGTTTTTATTAACAGCAATGCTT
ATAGGTTCTTTTTATAAGAAAAATGCAAGCATTAGCGGAGCAGAAGCAGGCTGTCAAGCAGAAATTGGTAGTGCTAGCTC
TATGGCAGCTGCAGCTATGGCAACAGTTTTAGGGGCTAATGCTTTTAAAGCATGCAACGCTGCTGAAATGGCAATGGAAC
ATCATCTAGGTTTAACTTGCGATCCAGTTGCAGGGCTTGTGCAAATTCCTTGCATAGAAAGAAATGCCTTTGGAGCTATT
AAGGCTATAAGTGCTGCTAGAATGGCAATGACACGTAAATCTACTCCTATGGTAAGCCTTGATGAGGTTATAGAAACCAT
GTATGAAACAGGAAAAGATATGAATTATAAATACAAAGAAACTTCTTTAGGTGGTCTTGCTACAAATTTAAAAACAGTAT
GCTAA

Upstream 100 bases:

>100_bases
CAAGATTTCGTAATAAATTTTTAGATCTTTTTATTTTGGTAATGGGGATAGTTGCAATTTCTGCTGCAATTCATGATCTT
TTATAAATTAAGGATAAAAA

Downstream 100 bases:

>100_bases
AGTGTTTTTACACTTTGGTGTATTTTTTCATTCTTAGACTTAAAAATTCTTTCATTTCTTCTTTGGAAAAACTTTTAAGA
ATACGAATTTGCTTAAAAAG

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSNLSIFKIGVGPSSSHTLGPMLAGNLFCKKVAKKLDEIDRVEVTLYGSLSLTGKGHLSDKAVIWGLNGLEAKNLSTAIQ
DEVNKNAIENAQIDFCGEKKLCFNYEKDLIFSKDFLPLHENGMKIKAYDCKGGLVDEETYYSVGGGFVLTAAQLEKKGKN
SNQNKKKKLDIELNNAKEALELCDKRDWDLAELSYRYELQFHTKEEIRAYCLEIWEVMQEVYYNGTHPNEDYLPGKLHLK
RRAKGLKERVAMTADPMGIIDFISLYAIAIAEENASGAKVVTAPTNGACAVIPAVMLYLKNHTIGFSDEKAIEFLLTAML
IGSFYKKNASISGAEAGCQAEIGSASSMAAAAMATVLGANAFKACNAAEMAMEHHLGLTCDPVAGLVQIPCIERNAFGAI
KAISAARMAMTRKSTPMVSLDEVIETMYETGKDMNYKYKETSLGGLATNLKTVC

Sequences:

>Translated_454_residues
MSNLSIFKIGVGPSSSHTLGPMLAGNLFCKKVAKKLDEIDRVEVTLYGSLSLTGKGHLSDKAVIWGLNGLEAKNLSTAIQ
DEVNKNAIENAQIDFCGEKKLCFNYEKDLIFSKDFLPLHENGMKIKAYDCKGGLVDEETYYSVGGGFVLTAAQLEKKGKN
SNQNKKKKLDIELNNAKEALELCDKRDWDLAELSYRYELQFHTKEEIRAYCLEIWEVMQEVYYNGTHPNEDYLPGKLHLK
RRAKGLKERVAMTADPMGIIDFISLYAIAIAEENASGAKVVTAPTNGACAVIPAVMLYLKNHTIGFSDEKAIEFLLTAML
IGSFYKKNASISGAEAGCQAEIGSASSMAAAAMATVLGANAFKACNAAEMAMEHHLGLTCDPVAGLVQIPCIERNAFGAI
KAISAARMAMTRKSTPMVSLDEVIETMYETGKDMNYKYKETSLGGLATNLKTVC
>Mature_453_residues
SNLSIFKIGVGPSSSHTLGPMLAGNLFCKKVAKKLDEIDRVEVTLYGSLSLTGKGHLSDKAVIWGLNGLEAKNLSTAIQD
EVNKNAIENAQIDFCGEKKLCFNYEKDLIFSKDFLPLHENGMKIKAYDCKGGLVDEETYYSVGGGFVLTAAQLEKKGKNS
NQNKKKKLDIELNNAKEALELCDKRDWDLAELSYRYELQFHTKEEIRAYCLEIWEVMQEVYYNGTHPNEDYLPGKLHLKR
RAKGLKERVAMTADPMGIIDFISLYAIAIAEENASGAKVVTAPTNGACAVIPAVMLYLKNHTIGFSDEKAIEFLLTAMLI
GSFYKKNASISGAEAGCQAEIGSASSMAAAAMATVLGANAFKACNAAEMAMEHHLGLTCDPVAGLVQIPCIERNAFGAIK
AISAARMAMTRKSTPMVSLDEVIETMYETGKDMNYKYKETSLGGLATNLKTVC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI48994925, Length=464, Percent_Identity=47.198275862069, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788116, Length=462, Percent_Identity=45.6709956709957, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1789161, Length=461, Percent_Identity=46.2039045553145, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 49578; Mature: 49446

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLSIFKIGVGPSSSHTLGPMLAGNLFCKKVAKKLDEIDRVEVTLYGSLSLTGKGHLSD
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCC
KAVIWGLNGLEAKNLSTAIQDEVNKNAIENAQIDFCGEKKLCFNYEKDLIFSKDFLPLHE
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCEEEECCCCCCCC
NGMKIKAYDCKGGLVDEETYYSVGGGFVLTAAQLEKKGKNSNQNKKKKLDIELNNAKEAL
CCCEEEEEECCCCCCCCCCEEECCCCEEEEHHHHHHCCCCCCCCCCEEEEEEECCHHHHH
ELCDKRDWDLAELSYRYELQFHTKEEIRAYCLEIWEVMQEVYYNGTHPNEDYLPGKLHLK
HHHCCCCCCHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RRAKGLKERVAMTADPMGIIDFISLYAIAIAEENASGAKVVTAPTNGACAVIPAVMLYLK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCEEEECCCCCCHHHHHHHHHHHH
NHTIGFSDEKAIEFLLTAMLIGSFYKKNASISGAEAGCQAEIGSASSMAAAAMATVLGAN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
AFKACNAAEMAMEHHLGLTCDPVAGLVQIPCIERNAFGAIKAISAARMAMTRKSTPMVSL
HHHHCCHHHHHHHHCCCCCCCCCCCCEECCEECCCCCHHHHHHHHHHHHHHHCCCCCEEH
DEVIETMYETGKDMNYKYKETSLGGLATNLKTVC
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
SNLSIFKIGVGPSSSHTLGPMLAGNLFCKKVAKKLDEIDRVEVTLYGSLSLTGKGHLSD
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCC
KAVIWGLNGLEAKNLSTAIQDEVNKNAIENAQIDFCGEKKLCFNYEKDLIFSKDFLPLHE
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCEEEECCCCCCCC
NGMKIKAYDCKGGLVDEETYYSVGGGFVLTAAQLEKKGKNSNQNKKKKLDIELNNAKEAL
CCCEEEEEECCCCCCCCCCEEECCCCEEEEHHHHHHCCCCCCCCCCEEEEEEECCHHHHH
ELCDKRDWDLAELSYRYELQFHTKEEIRAYCLEIWEVMQEVYYNGTHPNEDYLPGKLHLK
HHHCCCCCCHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RRAKGLKERVAMTADPMGIIDFISLYAIAIAEENASGAKVVTAPTNGACAVIPAVMLYLK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCEEEECCCCCCHHHHHHHHHHHH
NHTIGFSDEKAIEFLLTAMLIGSFYKKNASISGAEAGCQAEIGSASSMAAAAMATVLGAN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
AFKACNAAEMAMEHHLGLTCDPVAGLVQIPCIERNAFGAIKAISAARMAMTRKSTPMVSL
HHHHCCHHHHHHHHCCCCCCCCCCCCEECCEECCCCCHHHHHHHHHHHHHHHCCCCCEEH
DEVIETMYETGKDMNYKYKETSLGGLATNLKTVC
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]