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The map label for this gene is hisF-2

Identifier: 57238616

GI number: 57238616

Start: 1672090

End: 1672857

Strand: Direct

Name: hisF-2

Synonym: CJE1775

Alternate gene names: 57238616

Gene position: 1672090-1672857 (Clockwise)

Preceding gene: 57238615

Following gene: 57238617

Centisome position: 94.05

GC content: 32.94

Gene sequence:

>768_bases
ATGCTTACAAAACGCATAATTGCATGTTTAGATGTCAAAGATGGCAGAGTAGTAAAAGGAACACAATTTAAAAACCATAA
AGATATGGGAGATATCATAGAGCTTGCTAGGTATTATTCTCAAAATGGTATTGATGAGCTTGTTTTTTATGATATTGCTG
CAAGTGCTAGAAAAGAACGCATATCAAGAGAATGGGTAAGTGAAGTAGCAAAAAATATCAATATTTCTTTTTGTGTTGCA
GGGGGTATTAAAAGCGAAGAAGATGCAGCTGAACTTTTGGCTAATGGGGCAGATAAAATTTCTATCAATTCTCCTGCCTT
AAATGATCCAAGCCTAATCACACGCCTTGCTAAAAGCTTTGGCGTGCAATGCGTAGTAGTAGGGATTGATAGTTTTAAAG
ATGAAAATGGAAATTTAAAAGTATTTCAATACACAGGGGATGAAAAAACAAGTAAACATAGTGGAAAAAGCACTTTAGAA
TGGGTTAAAAAAGTGCAAGATCTAGGTGCAGGCGAAATTGTTTTAAATATGATGAATCAAGATGGTGTTAAAAATGGCTA
TGATTTAGAACAATTAGAAGCTGTATATAAAATTTGTAAAGTACCTTTGATCGCAAGTGGGGGAGCGGGAAAAATGGAAC
ATTTTTTAGAAGCTTTTAAACTAGGTATAGATGGGGCTTTAGCTGCTTCTGTTTTTCATCAAAAACTTATAGACATTAAA
GAATTAAAAATTTATCTTAAAAATCAAGGCTTAAGTATAAGGATATAA

Upstream 100 bases:

>100_bases
AGTGGGCAAAGAAGCAAAAATTCAAGCTAATTTTGATGAAATTAAAAGCGATGAAGAAATGGAAAAATATCTTAAAGAAA
AATGTATTTTGGAGAAATAA

Downstream 100 bases:

>100_bases
TATGCAAAATTTCAAAGAACTTAATGAAAAAATCGCTTGGCAAAAAGTGGATAATCTTTTACCTGTCATCATCCAAGATG
CAAAAACTTGCGAAGTTTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINISFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKKVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKVPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI

Sequences:

>Translated_255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINISFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKKVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKVPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI
>Mature_255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINISFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKKVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKVPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=65.7587548638132, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=24.5833333333333, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=32.484076433121, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS61_CAMJE (Q9PM72)

Other databases:

- EMBL:   AL111168
- PIR:   A81256
- RefSeq:   YP_002344972.1
- ProteinModelPortal:   Q9PM72
- SMR:   Q9PM72
- IntAct:   Q9PM72
- GeneID:   905871
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj1603
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- PhylomeDB:   Q9PM72
- ProtClustDB:   PRK02083
- BioCyc:   CJEJ192222:CJ1603-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28060; Mature: 28060

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKER
CCHHHEEHEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ISREWVSEVAKNINISFCVAGGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSF
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLEWVKKVQDLGAGEIVLNMMNQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
DGVKNGYDLEQLEAVYKICKVPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
CCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELKIYLKNQGLSIRI
HHHHEEECCCCEEEC
>Mature Secondary Structure
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKER
CCHHHEEHEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ISREWVSEVAKNINISFCVAGGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSF
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLEWVKKVQDLGAGEIVLNMMNQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
DGVKNGYDLEQLEAVYKICKVPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
CCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELKIYLKNQGLSIRI
HHHHEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204